SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
rnpA recombinant protein
Recombinant E Coli rnpA protein
Gene Names
rnpA; ECK3696; JW3681
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
rnpA; Recombinant E Coli rnpA protein; ECK3696; Rnase P protein; RnaseP protein; b3704; JW3681; Ribonuclease P protein component; EC 3.1.26.5; Protein C5; rnpA recombinant protein
Host
E Coli
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid. Phosphate-Buffered Saline (pH7.4) containing 10% Glycerol
Concentration
0.25mg/ml (determined by absorbance at 280nm) (varies by lot)
Sequence Positions
1-119aa
Sequence
MVKLAFPREL RLLTPSQFTF VFQQPQRAGT PQITILGRLN SLGHPRIGLT VAKKNVRRAH ERNRIKRLTR ESFRLRQHEL PAMDFVVVAK KGVADLDNRA LSEALEKLWR RHCRLARGS
Applicable Applications for rnpA recombinant protein
SDS-PAGE
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Tag
Non-Tagged
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for rnpA recombinant protein
rnpA, also known as Rnase P protein, is an essential enzyme consisting of the C5 protein (encoded by rnpA) and the catalytic M1 RNA (encoded by rnpB) subunits. rnpA is ribonucleoprotein that catalyzes the removal of the 50-leader elements of precursor tRNAs and generates the mature 50-end of tRNAs. This step is essential for the formation of functional tRNA molecules in bacteria, archaea and eukarya. More importantly, it has recently been demonstrated that RNase P is required for the endonucleolytic separation of certain polycistronic tRNA transcripts such as valV valW, leuQ leuP leuV and secG leuU. Thus, it was hypothesized that the essential function of RNase P might be related to the complete absence of a particular tRNAthat was dependent on the enzyme for initial separation from polycistronic transcripts. Recombinant E.coli rnpA, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for rnpA recombinant protein
References
Ankit Agrawal., et al. (2014) Nucleic Acids Res. 42(17):11166-79. Hui-Woog Choe., et al. (2002) Acta Crystallogr D Biol Crystallogr. 59(Pt 2):350-2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,789 Da
NCBI Official Full Name
protein C5 component of RNase P
NCBI Official Symbol
rnpA
NCBI Official Synonym Symbols
ECK3696; JW3681
NCBI Protein Information
protein C5 component of RNase P
UniProt Protein Name
Ribonuclease P protein component
Protein Family
UniProt Gene Name
rnpA
UniProt Synonym Gene Names
RNase P protein; RNaseP protein
NCBI Description
Has leadzyme properties. [More information is available at EcoGene: EG10862]. RnpB RNA and RnpA protein together compose ribonuclease P (RNAse P) . [More information is available at EcoCyc: EG10862].
Uniprot Description
RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.
Research Articles on rnpA
Similar Products
Product Notes
The rnpA rnpa (Catalog #AAA201367) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-119aa. AAA Biotech's rnpA can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the rnpA rnpa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MVKLAFPREL RLLTPSQFTF VFQQPQRAGT PQITILGRLN SLGHPRIGLT VAKKNVRRAH ERNRIKRLTR ESFRLRQHEL PAMDFVVVAK KGVADLDNRA LSEALEKLWR RHCRLARGS. It is sometimes possible for the material contained within the vial of "rnpA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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