TSLP recombinant protein

Recombinant TSLP

Cat. Num. AAA201144

• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: TSLP; Recombinant TSLP; Thymic stromal lymphopoietin; Thymic stroma-derived lymphopoietin; Tslp; TSLP recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 20-140aa
• Sequence: ADP YNFSNCN FTSITKIYCN IIFHDLTGDL KGAKFEQIED CESKPACLLK IEYYTLNPIP GCPSLPDKTF ARRTREALND HCPGYPETER NDGTQEMAQE VQNICLNQTS QILRLWYSFM QSPE HHHHHH
• Sequence Length: 140

• Applicable Applications for TSLP recombinant protein: SDS-PAGE
• Species: Mouse
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Predicted N-Terminal: Ala
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for TSLP recombinant protein

TSLP, also known as thymic stromal lymphopoietin, is a protein belonging to the cytokine family. This protein plays an important role in the maturation of T cell populations through activation of antigen presenting cells. It is an interleukin 7-like cytokine originally characterized by its ability to promote the activation of B cells and dendritic cells (DCs). It is expressed by non-hematopoietic cells such as fibroblasts, epithelial cells and different types of stromal or stromal-like cells, and is important in allergic inflammation. It signals through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor CRLF2 and the IL-7R alpha chain. After binding STAT5 phosphorylation is induced resulting in the expression of upstream transcription factors. Also, expression of TSLP is linked to many disease states including asthma, inflammatory arthritis, atopic dermatitis, eczema, eosinophilic esophagitis and other allergic states. But the factors are not clearly defined. Recombinant Mouse TSLP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for TSLP recombinant protein

Immunology

References

Sims JE., et al, (2000) J. Exp. Med. 192:671-680. Pandey A., et al, (2000) Nautre Immunol. 1:59.

NCBI and Uniprot Product Information

• NCBI GI #: 81868532
• NCBI GeneID: 53603
• UniProt Accession #: Q9JIE6
• Molecular Weight: 15.0kDa (130aa); 18-28kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: Thymic stromal lymphopoietin
• NCBI Official Synonym Full Names: thymic stromal lymphopoietin
• NCBI Official Symbol: Tslp
• NCBI Protein Information: thymic stromal lymphopoietin
• UniProt Protein Name: Thymic stromal lymphopoietin
• Protein Family: Thymic stromal lymphopoietin
• UniProt Gene Name: Tslp
• UniProt Entry Name: TSLP_MOUSE

Uniprot Description

TSLP: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: cytokine activity