H3N2-HA1 recombinant protein

Recombinant H3N2-HA1

Cat. Num. AAA201065

• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: H3N2-HA1; Recombinant H3N2-HA1; Hemagglutinin; H3N2-HA1 recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 18-344aa
• Sequence: ADP NLPGNEN NAATLCLGHH AVPNGTIVKT ITDDQIEVTN ATELVQNSST GKICNNPHKI LDGRDCTLID ALLGDPHCDV FQNETWDLFV ERSNAFSNCY PYDVPDYASL RSIVASSGTL EFITEGFTWA GVTQNGGSGA CKRGPANGFF SRLNWLTKSG NTYPVLNVTM PNNNNFDKLY IWGVHHPSTN QEQTSLYIQA SGRVTVSTRR SQQTIIPNIG SRPLVRGQSG RISVYWTIVK PGDVLVINSN GNLIAPRGYF KMRIGKSSIM RSDAPIDTCI SECITPNGSI PNEKPFQNVN KITYGACPKY VKQNTLKLAT GMRNVPEKQT HHHHHH

• Applicable Applications for H3N2-HA1 recombinant protein: SDS-PAGE
• Species: Influenza A virus (H3N2, Canine)
• Endotoxin: <1.0 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Predicted N-Terminal: Ala
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for H3N2-HA1 recombinant protein

H3N2-HA1, also known as hemagglutinin antigen, is a subtype of viruses that causes influenza (flu). H3N2 Viruses can infect birds and mammals. In birds, humans, and pigs, the virus has mutated into many strains. It is increasingly abundant in seasonal influenza. This protein binds to sialic acid receptors on the cell surface, allowing viral particles to attach to cells. It plays an important role in host range restriction and toxicity determination, and mediates the penetration of virus into the cytoplasm by mediating the fusion of endosomal membrane and membrane. Recombinant Influenza A virus (H3N2, canine) HA protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for H3N2-HA1 recombinant protein

Virus

References

Rene Gramer., et al. (2007) Can J Vet Res. 71:201-206. Blanton., et al. (2017) MMWR Morb Mortal Wkly. 66:1043-1051.

NCBI and Uniprot Product Information

• UniProt Accession #: E2E3B0
• Molecular Weight: 36.9kDa (336aa)
• UniProt Protein Name: Hemagglutinin
• UniProt Gene Name: HA

Uniprot Description

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.