Ribonuclease A/RNASE 1 Recombinant Protein | RNASE1 recombinant protein

Recombinant human Ribonuclease A/RNASE 1 protein

Cat. Num. AAA201364

• Gene Names: RNASE1; RIB1; RNS1
• Applications: SDS-Page
• Purity: 90% by SDS-PAGE
• Synonyms: Ribonuclease A/RNASE 1; Recombinant human Ribonuclease A/RNASE 1 protein; RNASE1; ribonuclease A family member 1; pancreatic; RAC1; RIB1; RNS1; ribonuclease pancreatic; HP-RNase; RIB-1; RNase UpI-1; RNase 1; Ribonuclease A; RNase A; RNASE1 recombinant protein

• Host: HEK293
• Purity/Purification: 90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 29-156aa
• Sequence: KESRAKKFQR QHMDSDSSPS SSSTYCNQMM RRRNMTQGRC KPVNTFVHEP LVDVQNVCFQ EKVTCKNGQG NCYKSNSSMH ITDCRLTNGS RYPNCAYRTS PKERHIIVAC EGSPYVPVHF DASVEDST

• Applicable Applications for RNASE1 recombinant protein: SDS-PAGE, Enzyme Activity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Lys29
• Bioactivity: Specific activity is > 3 X 10^6 unit/mg, and is defined as the amount of enzyme that cleaves 1.0 pmole of RNase probe per minute at 25 degree C.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for RNASE1 recombinant protein

RNASE1, also known as ribonuclease A, is a member of pancreatic ribonuclease enzyme family. It is a relatively small protein and is a basic protein (pI = 9.63). Also, It has four disulfide bonds in its native state. It cleaves specifically after pyrimidine nucleotides. Cleavage takes place in two steps: first, the 3',5'-phosphodiester bond is cleaved to generate a 2',3'-cyclic phosphodiester intermediate; second, the cyclic phosphodiester is hydrolyzed to a 3'-monophosphate. (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG) The highest activity is exhibited with single stranded RNA. It can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. Recombinant human RNASE1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
N-terminal Sequence Analysis: Lys-Glu-Ser-Arg-Ala

Product Categories/Family for RNASE1 recombinant protein

Enzymes & Proteases; Epigenomics (Transcription & Translation); Proteins

References

Raines RT (1998) Chem. Rev. 98:1045-1066. JC Kosgey et al, (2020) Int. J. Biol. 160:1042-1049.

NCBI and Uniprot Product Information

• NCBI GI #: 38201686
• NCBI GeneID: 6035
• NCBI Accession #: NP_937878.1
• NCBI GenBank Nucleotide #: NM_198235.2
• UniProt Accession #: P07998; Q16830; Q16869; Q1KHR2; Q6ICS5; Q9UCB4; Q9UCB5; B2R589; D3DS06
• Molecular Weight: 17,644 Da
• NCBI Official Full Name: ribonuclease pancreatic
• NCBI Official Synonym Full Names: ribonuclease, RNase A family, 1 (pancreatic)
• NCBI Official Symbol: RNASE1
• NCBI Official Synonym Symbols: RIB1; RNS1
• NCBI Protein Information: ribonuclease pancreatic; RIB-1; RNase 1; RNase A; HP-RNase; RNase upI-1; ribonuclease 1; ribonuclease A
• UniProt Protein Name: Ribonuclease pancreatic
• Protein Family: Ribonuclease pancreatic
• UniProt Gene Name: RNASE1
• UniProt Synonym Gene Names: RIB1; RNS1; RNase 1; RNase A

NCBI Description

This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA.