SPARC recombinant protein

Recombinant mouse SPARC protein

Cat. Num. AAA201259

• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: SPARC; Recombinant mouse SPARC protein; Basement-membrane protein 40; BM-40;Osteonectin; ON; Secreted protein acidic and rich in cysteine; Sparc; SPARC recombinant protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 18-302aa
• Sequence: APQQTEVAEE IVEEETVVEE TGVPVGANPV QVEMGEFEDG AEETVEEVVA DNPCQNHHCK HGKVCELDES NTPMCVCQDP TSCPAPIGEF EKVCSNDNKT FDSSCHFFAT KCTLEGTKKG HKLHLDYIGP CKYIAPCLDS ELTEFPLRMR DWLKNVLVTL YERDEGNNLL TEKQKLRVKK IHENEKRLEA GDHPVELLAR DFEKNYNMYI FPVHWQFGQL DQHPIDGYLS HTELAPLRAP LIPMEHCTTR FFETCDLDND KYIALEEWAG CFGIKEQDIN KDLVI

• Applicable Applications for SPARC recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Mouse
• Tag: His-Tag
• Predicted N-term: Ala
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for SPARC recombinant protein

SPARC, also known as osteonectin and BM40, is a secreted matricellular glycoprotein that belongs to a group of functionally related glycoproteins that includes tenascins C and X, thrombospondins 1 and 2, and osteopontin. It is a major bone-related protein that is also present in nonmineralized tissues and in platelets and is matricellular molecule regulating interactions between cells and their surrounding extracellular matrix (ECM). This protein is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. It shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation. It is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis. Recombinant mouse SPARC, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for SPARC recombinant protein

Cell Cycle; Proteins

References

Mizuho Fukunaga-Kalabi., et al, (2008) Cancer Microenviron. 1:93-102. Katarina Kos., et al, (2010) Nat Rev Endocrinol. 6:225-235. Sharon L I Wong., et al, (2017) Br J Pharmacol. 174:3-14.

NCBI and Uniprot Product Information

• UniProt Accession #: P07214
• Molecular Weight: 33.3kDa (291aa)
• UniProt Protein Name: SPARC
• Protein Family: SPARC
• UniProt Gene Name: Sparc
• UniProt Synonym Gene Names: BM-40; ON

Uniprot Description

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.