SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
Pentraxin2/SAP Recombinant Protein | SAP recombinant protein
Recombinant Pentraxin2/SAP
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
Pentraxin2/SAP; Recombinant Pentraxin2/SAP; Serum amyloid P-component; SAP; Apcs; Sap; SAP recombinant protein
Host
HEK293
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
21-224aa
Sequence
QTDLKRKVFV FPRESETDHV KLIPHLEKPL QNFTLCFRTY SDLSRSQSLF SYSVKGRDNE LLIYKEKVGE YSLYIGQSKV TVRGMEEYLS PVHLCTTWES SSGIVEFWVN GKPWVKKSLQ REYTVKAPPS IVLGQEQDNY GGGFQRSQSF VGEFSDLYMW DYVLTPQDIL FVYRDSPVNP NILNWQALNY EINGYVVIRP RVWDHHHHHH
Sequence Length
224
Applicable Applications for SAP recombinant protein
SDS-PAGE
Species
Mouse
Endotoxin
<1.0 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Predicted N-Terminal
Gln 21
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
Related Product Information for SAP recombinant protein
Pentraxin2, also known as serum amyloid P-component, is a secreted serum glycoprotein that belongs to the pentraxins family. This family binds calcium-dependent lectin-like manner and is characterized distinctive flattened Beta-jellyroll structure similar to the legume lectins. This protein is a non-fibrillar component, it can interact with DNA and histones. It regulates the solubility of amyloid fibrils and protects them from degradation by proteolytic enzymes and phagocytic cells. This protein is the major acute-phase protein and expression of it is dependent on complement activation, IL-6 and/or IL-1 beta. Recombinant mouse Pentraxin2 protein, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.
Product Categories/Family for SAP recombinant protein
References
Xi D., et al, (2015) Int J Cardiol. 187:20-26. Muczynski V., et al, (2017) Blood. 101(9): 129(17):2443-2454.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
24.6kDa (210aa); 28-40KDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
Serum amyloid P-component
UniProt Protein Name
Serum amyloid P-component
Protein Family
UniProt Gene Name
Apcs
UniProt Synonym Gene Names
Ptx2; Sap; SAP
UniProt Entry Name
SAMP_MOUSE
Uniprot Description
APCS: Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. Belongs to the pentaxin family.
Protein type: Secreted; Secreted, signal peptide; Chaperone
Cellular Component: extracellular matrix; extracellular space; protein complex; extracellular region; nucleus
Molecular Function: metal ion binding; complement component C1q binding; virion binding; calcium ion binding; carbohydrate binding
Biological Process: negative regulation of virion penetration into host cell; innate immune response; protein complex assembly; negative regulation of monocyte differentiation; negative regulation of viral reproduction
Similar Products
Product Notes
The SAP apcs (Catalog #AAA201123) is a Recombinant Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 21-224aa. AAA Biotech's Pentraxin2/SAP can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SAP apcs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QTDLKRKVFV FPRESETDHV KLIPHLEKPL QNFTLCFRTY SDLSRSQSLF SYSVKGRDNE LLIYKEKVGE YSLYIGQSKV TVRGMEEYLS PVHLCTTWES SSGIVEFWVN GKPWVKKSLQ REYTVKAPPS IVLGQEQDNY GGGFQRSQSF VGEFSDLYMW DYVLTPQDIL FVYRDSPVNP NILNWQALNY EINGYVVIRP RVWDHHHHHH. It is sometimes possible for the material contained within the vial of "Pentraxin2/SAP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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