Thrombospondin-4 Recombinant Protein | Thbs4 recombinant protein

Recombinant mouse Thrombospondin-4 protein

Cat. Num. AAA201348

• Gene Names: Thbs4; TSP4
• Applications: SDS-Page
• Purity: >85% by SDS-PAGE
• Synonyms: Thrombospondin-4; Recombinant mouse Thrombospondin-4 protein; THBS4; Thbs4; Thbs-4; Thrombospondin4; thrombospondin 4; Tsp4; TSP-4; TS; TSP; TSP4; Thbs4 recombinant protein

• Host: HEK293
• Purity/Purification: >85% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 0.1mM PMSF, 20% glycerol
• Concentration: 0.25mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 27-963aa
• Sequence: QATPQVFDLL PSSSQRLNPS ALQPVLTDPT LHEVYLISTF KLQSKSSATI FGLYSSSDNS KYFEFTVMGR LNKAILRYLK NDGKIHLVVF NNLQLADGRR HRVLLRLSNL QRGDGSVELY LDCAQADSVR NLPRAFSGLT QNPESIELRT FQRKPQDFLE ELKLVVRGSL FQVASLQDCF LQQSEPLAAT STGDFNRQFL GQMTQLNQLL GEVKDLLRQQ VKETSFLRNT IAECQACGPL SFQSPTPNTL VPIAPPAPPT RPTRHCDSSP CFRGVRCTDT RDGFQCGPCP DGYTGNGITC SDVDECKYHP CYPGVRCVNL APGFRCDACP VGFTGPMVQG VGINFAKTNK QVCTDVDECQ NGACVLNSIC INTLGSYRCG PCKPGYTGDQ TRGCKTERSC RNPEQNPCSV HAQCIEERQG DVTCVCGVGW AGDGYVCGKD VDIDSYPDEE LPCSARNCKK DNCKYVPNSG QEDADRDGIG DACDEDADGD GILNEQDNCV LTHNIDQRNS DKDIFGDACD NCRMVLNNDQ KDTDGDGRGD ACDDDMDGDG IKNILDNCPR VPNRDQQDRD GDDVGDACDS CPDVSNPNQS DVDNDLVGDS CDTNQDSDGD GHQDSTDNCP TVINSSQLDT DKDGIGDECD DDDDNDGIPD LVPPGPDNCR LVPNPAQEDS NNDGVGDICE ADFDQDQVID HIDVCPENAE ITLTDFRAYQ TVVLDPEGDA QIDPNWVVLN QGMEIVQTMN SDPGLAVGYT AFNGVDFEGT FHVNTQTDDD YAGFIFGYQD SSSFYVVMWK QTEQTYWQAT PFRAVAEPGI QLKAVKSKTG PGEHLRNSLW HTGDTSDQVR LLWKDSRNVG WKDKVSYRWF LQHRPQVGYI RVRFYEGSEL VADSGVTIDT TMRGGRLGVF CFSQENIIWS NLKYRCNDTI PEDFQEFQTQ SFDRLDN

• Applicable Applications for Thbs4 recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Mouse
• Tag: His-Tag
• Predicted N-term: Gln27
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for Thbs4 recombinant protein

Thrombospondin-4, also known as THBS4, is a member of the thrombospondin protein family. This family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentamer and can bind to heparin and calcium. Among them Thrombospondin-4 binds a variety of matrix proteins including Collagens I, II, III, V, Laminin?1, Fibronectin, and Matrilin?2. And it plays a role in cardiovascular physiology and neuronal development. It is up?regulated in the spinal cord following peripheral nerve injury where it contributes to presynaptic hypersensitivity and hyperalgesia. It is also up?regulated in muscle following denervation. It contributes to the development of inflammation and atherosclerosis by promoting macrophage and neutrophil adhesion to the vasculature. Recombinant mouse Thrombospondin-4, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for Thbs4 recombinant protein

Angiogenesis; Cardiovascular; Adhesion & Movement; Proteins

References

Lawler, J. et al. (1995) J. Biol. Chem. 270:2809-2814. Narouz-Ott, L. et al. (2000) J. Biol. Chem. 275:37110-37117. Arber, S. and P. Caroni (1995) J. Cell Biol. 131:1083-1094. Muppala S. et al.(2015) Arteriosclerosis. 1975-1986.

NCBI and Uniprot Product Information

• NCBI GI #: 6755781
• NCBI GeneID: 21828
• NCBI Accession #: NP_035712.1
• NCBI GenBank Nucleotide #: NM_011582.3
• UniProt Accession #: Q9Z1T2; Q9QYS3; Q9WUE0
• Molecular Weight: 106,366 Da
• NCBI Official Full Name: thrombospondin-4
• NCBI Official Synonym Full Names: thrombospondin 4
• NCBI Official Symbol: Thbs4
• NCBI Official Synonym Symbols: TSP4
• NCBI Protein Information: thrombospondin-4
• UniProt Protein Name: Thrombospondin-4
• Protein Family: Thrombospondin
• UniProt Gene Name: Thbs4
• UniProt Synonym Gene Names: Tsp4
• UniProt Entry Name: TSP4_MOUSE

Uniprot Description

THBS4: Acts as an extracellular mitogen and stimulates early erythroid progenitors proliferation in presence of EPO. Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen. Belongs to the thrombospondin family.

Protein type: Motility/polarity/chemotaxis

Cellular Component: basement membrane; endoplasmic reticulum; extracellular matrix; extracellular region; extracellular space; neuromuscular junction; proteinaceous extracellular matrix; sarcoplasmic reticulum

Molecular Function: calcium ion binding; collagen binding; fibronectin binding; growth factor activity; heparin binding; integrin binding; laminin-1 binding; protein binding

Biological Process: behavioral response to pain; cell adhesion; myoblast migration; nervous system development; neurite morphogenesis; positive regulation of cell division; positive regulation of endothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; protein homooligomerization; regulation of tissue remodeling; response to unfolded protein; tissue remodeling