Western Blot (WB)
(Staining (1ug/ml) of Human Kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat VPS26A Polyclonal Antibody | anti-VPS26A antibody
Goat anti-VPS26A Antibody
Western Blot: Approx 40kDa band observed in Human Kidney lysates (calculated MW of 38.2 kDa according to NP_004887.2). In transfected HEK293 transiently expressing VPS26A a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 1-3ug/ml.
Western Blot (WB)
(Staining (1ug/ml) of Human Kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (1ug/ml) of Human Kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing VPS26A (RC205353) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing VPS26A (RC205353) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
VPS26A: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Belongs to the VPS26 family.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 10q21.1
Cellular Component: cytosol; early endosome; endosome; endosome membrane; intracellular membrane-bound organelle; retromer complex; vesicle
Molecular Function: protein binding; protein transporter activity
Biological Process: intracellular protein transport; regulation of macroautophagy; retrograde transport, endosome to Golgi; Wnt receptor signaling pathway
Research Articles on VPS26A
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Product Notes
The VPS26A vps26a (Catalog #AAA420801) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-VPS26A Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's VPS26A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 40kDa band observed in Human Kidney lysates (calculated MW of 38.2 kDa according to NP_004887.2). In transfected HEK293 transiently expressing VPS26A a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 1-3ug/ml. Researchers should empirically determine the suitability of the VPS26A vps26a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SPESQASAEQ PEM. It is sometimes possible for the material contained within the vial of "VPS26A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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