IL-8/CXCL8 recombinant protein

Recombinant canine IL-8/CXCL8 protein

Cat. Num. AAA201356

• Gene Names: CXCL8; IL8
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: IL-8/CXCL8; Recombinant canine IL-8/CXCL8 protein; 3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Chemokine (C-X-C motif) ligand 8; Emoctakin; GCP-1TSG-1; GCP1; IL-8; IL8; Interleukin-8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; MONAP; MONAPGCP1; NAF; NAP-1NAP1; NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1; interleukin 8; member 8; IL-8/CXCL8 recombinant protein

• Host: HEK293
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 20% glycerol
• Concentration: 0.25mg/ml (determined by BCA assay) (varies by lot)
• Sequence Positions: 28-101aa
• Sequence: VSSELRCQCI KTHSTPFHPK YIKELRVIDS GPHCENSEII VKLFNGNEVC LDPKEKWVQK VVQIFLKKAE KQDP

• Applicable Applications for IL-8/CXCL8 recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Canine
• Tag: His-Tag
• Predicted N-term: Val 28
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for IL-8/CXCL8 recombinant protein

IL-8/CXCL8, also known as Interleukin-8, GCP-1, and NAP-1 is a member of the CXC family of chemokines family which is widely expressed proinflammatory. IL-8 is a potent neutrophil chemotactic factor. It attracts neutrophils, basophils, and T-cells, but not monocytes. This protein has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also stimulates phagocytosis once they have arrived. IL-8 is also known to be a potent promoter of angiogenesis. In target cells, IL-8 induces a series of physiological responses required for migration and phagocytosis, such as increases in intracellular Ca2+, exocytosis, and the respiratory burst. Among the eceptors on the surface membrane capable of binding IL-8, the most frequently studied receptors are CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2). Recombinant canine IL-8/CXCL8, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for IL-8/CXCL8 recombinant protein

Hormones & Cytokines; Angiogenesis; Cardiovascular; Proteins

References

Lazennec, G. and A. Richmond (2010) Trends Mol. Med. 16:133-144. Munoz, L.M. et al. (2009) J. Immunol. 183:7337-7346. Bergin, D.A. et al. (2010) J. Clin. Invest. 120:4236-4250.

NCBI and Uniprot Product Information

• NCBI GI #: 55741690
• NCBI GeneID: 403850
• NCBI Accession #: NP_001003200.1
• NCBI GenBank Nucleotide #: NM_001003200.1
• UniProt Accession #: P41324
• Molecular Weight: 11,280 Da
• NCBI Official Full Name: interleukin-8
• NCBI Official Symbol: CXCL8
• NCBI Official Synonym Symbols: IL8
• NCBI Protein Information: interleukin-8; C-X-C motif chemokine 8; IL-8; chemokine (C-X-C motif) ligand 8
• UniProt Protein Name: Interleukin-8
• UniProt Gene Name: CXCL8
• UniProt Synonym Gene Names: IL8; IL-8
• UniProt Entry Name: IL8_CANFA

Uniprot Description

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.