COMP recombinant protein

Recombinant human COMP protein

Cat. Num. AAA201350

• Gene Names: COMP; MED; EDM1; EPD1; PSACH; THBS5
• Applications: SDS-Page
• Purity: >85% by SDS-PAGE
• Synonyms: COMP; Recombinant human COMP protein; cartilage oligomeric matrix protein; Thrombospondin-5; EDM1; EPD1; MED; PSACH; THBS5; TSP5; CTS2; COMP recombinant protein

• Host: HEK293
• Purity/Purification: >85% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 21-757aa
• Sequence: QGQSPL GSDLGPQMLR ELQETNAALQ DVRELLRQQV REITFLKNTV MECDACGMQQ SVRTGLPSVR PLLHCAPGFC FPGVACIQTE SGARCGPCPA GFTGNGSHCT DVNECNAHPC FPRVRCINTS PGFRCEACPP GYSGPTHQGV GLAFAKANKQ VCTDINECET GQHNCVPNSV CINTRGSFQC GPCQPGFVGD QASGCQRRAQ RFCPDGSPSE CHEHADCVLE RDGSRSCVCA VGWAGNGILC GRDTDLDGFP DEKLRCPERQ CRKDNCVTVP NSGQEDVDRD GIGDACDPDA DGDGVPNEKD NCPLVRNPDQ RNTDEDKWGD ACDNCRSQKN DDQKDTDQDG RGDACDDDID GDRIRNQADN CPRVPNSDQK DSDGDGIGDA CDNCPQKSNP DQADVDHDFV GDACDSDQDQ DGDGHQDSRD NCPTVPNSAQ EDSDHDGQGD ACDDDDDNDG VPDSRDNCRL VPNPGQEDAD RDGVGDVCQD DFDADKVVDK IDVCPENAEV TLTDFRAFQT VVLDPEGDAQ IDPNWVVLNQ GREIVQTMNS DPGLAVGYTA FNGVDFEGTF HVNTVTDDDY AGFIFGYQDS SSFYVVMWKQ MEQTYWQANP FRAVAEPGIQ LKAVKSSTGP GEQLRNALWH TGDTESQVRL LWKDPRNVGW KDKKSYRWFL QHRPQVGYIR VRFYEGPELV ADSNVVLDTT MRGGRLGVFC FSQENIIWAN LRYRCNDTIP EDYETHQLRQ A

• Applicable Applications for COMP recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Asp
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for COMP recombinant protein

COMP, also known as Thrombospondin-5, is a member of the thrombospondin(TSP) family. It is an extracellular matrix(ECM) protein primarily present in cartilage and is a secreted glycoprotein that is important for growth plate organization and function. This protein is upregulated in rheumatoid osteoarthritis and arthritis, chronic pancreatitis, hepatocellular carcinomas and pancreatic carcinomas. It is a marker of cartilage turnover. It is present in high quantities in fibrotic scars and systemic sclerosis, and it appears to have a role in vascular wall remodeling. It also plays a role in cell growth and development. Mutations in COMP can cause the osteochondrodysplasias pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia (MED), and up-regulated expression of COMP are observed in rheumatoid arthritis and certain carcinomas. Recombinant human COMP, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for COMP recombinant protein

Signal Transduction; Cytoskeleton; Proteins

References

Wislowska M., et al, (2005) Clin. Rheumatol. 24:278-284. Hou J., et al, (2000) Cell Calcium. 27:309-314.

NCBI and Uniprot Product Information

• NCBI GI #: 40217843
• NCBI GeneID: 1311
• NCBI Accession #: NP_000086.2
• NCBI GenBank Nucleotide #: NM_000095.2
• UniProt Accession #: P49747; O14592; Q16388; Q16389; Q2NL86; Q8N4T2; B4DKJ3
• Molecular Weight: 77,214 Da
• NCBI Official Full Name: cartilage oligomeric matrix protein
• NCBI Official Synonym Full Names: cartilage oligomeric matrix protein
• NCBI Official Symbol: COMP
• NCBI Official Synonym Symbols: MED; EDM1; EPD1; PSACH; THBS5
• NCBI Protein Information: cartilage oligomeric matrix protein; TSP5; cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple); cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia 1, multiple); pseudoachondroplasia (epi
• UniProt Protein Name: Cartilage oligomeric matrix protein
• Protein Family: Complexin
• UniProt Gene Name: COMP
• UniProt Synonym Gene Names: COMP; TSP5
• UniProt Entry Name: COMP_HUMAN

NCBI Description

The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2008]

Uniprot Description

COMP: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COMP are the cause of pseudoachondroplasia (PSACH). PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. Belongs to the thrombospondin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: heparin binding; collagen binding; heparan sulfate proteoglycan binding; protein binding; protease binding; extracellular matrix structural constituent; calcium ion binding

Biological Process: limb development; extracellular matrix organization and biogenesis; organ morphogenesis; apoptosis; cell adhesion; skeletal development; negative regulation of apoptosis

Disease: Epiphyseal Dysplasia, Multiple, 1; Pseudoachondroplasia