SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Osteopontin/OPN Active Protein | OPN active protein
Recombinant mouse Osteopontin/OPN protein
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
Osteopontin/OPN; Recombinant mouse Osteopontin/OPN protein; secreted phosphoprotein 1; 2AR; Ap1-1; BNSP; Bsp; BSP1; Eta; ETA-1; mi; OP; Opn; Opn1; Spp-1; osteopontine isoform 4; Bone sialoprotein 1; Calcium oxalate crystal growth inhibitor protein; Early T-lymphocyte activation 1 protein; Minopontin; Osteoponitin; Spp1; OPN active protein
Host
HEK293
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
17-294aa
Sequence
Applicable Applications for OPN active protein
SDS-PAGE, Bioactivity
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Species
Mouse
Tag
His-Tag
Predicted N-term
Asp
Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When cells are added to mouse SPP1 coated plates. The ED50 range <= 1.5ug/ml.
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Biological Activity
(Mouse SPP1 stimulates cell adhesion of the HEK293 human embryonic kidney cells. The ED50 range )
Biological Activity
(Mouse SPP1 stimulates cell adhesion of the HEK293 human embryonic kidney cells. The ED50 range )
Related Product Information for OPN active protein
Osteopontin, also known as SPP1, is major non-collagenous bone protein that binds tightly to hydroxyapatite. This protein appears to form an integral part of the mineralized matrix. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell ad-hesion and migration. Osteopontin acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. Also it is essential in the pathway that leads to type 1 immunity. It is expressed and secreted by various cells, and has a role in cell adhesion, chemotaxis, prevention of apoptosis, invasion, migration and anchorage-independent growth of tumor cells. Recombinant mouse Osteopontin, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Categories/Family for OPN active protein
References
Icer MA, et al, (2018) Clin Biochem. 51:17-24. Kaleta B. (2019) Inflamm Res. 68:93-102. Lamort AS, et al, (2019) Cells. 8:815.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
31.8kDa (287aa)
UniProt Protein Name
Osteopontin
UniProt Gene Name
Spp1
UniProt Synonym Gene Names
Eta-1; Op; Spp-1; SPP-1
UniProt Entry Name
OSTP_MOUSE
Uniprot Description
osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; cell projection; perinuclear region of cytoplasm; apical part of cell; cytoplasm; extracellular region
Molecular Function: extracellular matrix binding; cytokine activity
Biological Process: response to organic substance; osteoblast differentiation; neutrophil chemotaxis; ossification; response to vitamin D; response to steroid hormone stimulus; biomineral formation; positive regulation of bone resorption; cell adhesion; negative regulation of apoptosis
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Product Notes
The OPN spp1 (Catalog #AAA201365) is an Active Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 17-294aa. AAA Biotech's Osteopontin/OPN can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Bioactivity. Researchers should empirically determine the suitability of the OPN spp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below:Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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