DLL4 recombinant protein

Recombinant human DLL4 protein

Cat. Num. AAA201267

• Gene Names: DLL4; hdelta2
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: DLL4; Recombinant human DLL4 protein; Delta-like protein 4; Drosophila Delta homolog 4; Delta4; delta like canonical Notch ligand 4;AOS6; hdelta2; DLL4 recombinant protein

• Host: HEK293
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 27-524aa
• Sequence: SGVFQLQLQE FINERGVLAS GRPCEPGCRT FFRVCLKHFQ AVVSPGPCTF GTVSTPVLGT NSFAVRDDSS GGGRNPLQLP FNFTWPGTFS LIIEAWHAPG DDLRPEALPP DALISKIAIQ GSLAVGQNWL LDEQTSTLTR LRYSYRVICS DNYYGDNCSR LCKKRNDHFG HYVCQPDGNL SCLPGWTGEY CQQPICLSGC HEQNGYCSKP AECLCRPGWQ GRLCNECIPH NGCRHGTCST PWQCTCDEGW GGLFCDQDLN YCTHHSPCKN GATCSNSGQR SYTCTCRPGY TGVDCELELS ECDSNPCRNG GSCKDQEDGY HCLCPPGYYG LHCEHSTLSC ADSPCFNGGS CRERNQGANY ACECPPNFTG SNCEKKVDRC TSNPCANGGQ CLNRGPSRMC RCRPGFTGTY CELHVSDCAR NPCAHGGTCH DLENGLMCTC PAGFSGRRCE VRTSIDACAS SPCFNRATCY TDLSTDTFVC NCPYGFVGSR CEFPVGLP

• Applicable Applications for DLL4 recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Ser
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for DLL4 recombinant protein

DLL4, also known as Delta-Like protein 4, is a membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. It is predicted to encode a membrane-bound ligand, characterized by an extracellular region containing several EGF-like domains and a DSL domain required for receptor binding. DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. The Notch signaling pathway is fundamental to proper cardiovascular development and is now recognized as an important player in tumor angiogenesis. Two key Notch ligands have been implicated in tumor angiogenesis, Delta-like 4 and Jagged1. Recombinant human DLL4, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for DLL4 recombinant protein

Neuroscience; Proteins

References

J R Shutter., et al, (2000) Genes Dev. 14:1313-1318. J Dufraine., et al, (2008) Oncogene. 27:5132-5137.

NCBI and Uniprot Product Information

• NCBI GI #: 9506545
• NCBI GeneID: 54567
• NCBI Accession #: NP_061947
• NCBI GenBank Nucleotide #: NM_019074
• UniProt Accession #: Q9NR61; Q3KP23; Q9NQT9
• Molecular Weight: 74,605 Da
• NCBI Official Full Name: delta-like protein 4
• NCBI Official Synonym Full Names: delta-like 4 (Drosophila)
• NCBI Official Symbol: DLL4
• NCBI Official Synonym Symbols: hdelta2
• NCBI Protein Information: delta-like protein 4; delta 4; delta ligand 4; delta-like 4 homolog; delta-like 4 protein; delta4; drosophila Delta homolog 4; notch ligand DLL4; notch ligand delta-2
• UniProt Protein Name: Delta-like protein 4
• Protein Family: Delta-like protein
• UniProt Gene Name: DLL4
• UniProt Synonym Gene Names: Delta4

Uniprot Description

Involved in the Notch signaling pathway as Notch ligand (PubMed:11134954). Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting (PubMed:20616313). Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (). During spinal cord neurogenesis, inhibits V2a interneuron fate (PubMed:17728344).