Typical Testing Data/Standard Curve (for reference only)
Rat Sclerostin ELISA Kit | SOST elisa kit
Rat Sclerostin ELISA Kit
Reactivity
Rat
Synonyms
Sclerostin; Rat Sclerostin ELISA Kit; SOST elisa kit
Reactivity
Rat
Specificity
The Rat Sclerostin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat Sclerostin proteins within the range of 15.6 pg/ml - 1000 pg/ml.
Sequence Length
213
Samples
Cell culture supernates, serum, plasma
Sensitivity
< 10 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SOST elisa kit
Principle of the Assay: The MyBioSource' Rat Sclerostin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Sclerostin in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Rat Sclerostin coated on a 96-well plate. Standards and samples are pipetted into the wells and Sclerostin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Sclerostin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Sclerostin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Sclerostin is a protein that in humans is encoded by the SOST gene. It is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Additionally, Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21.
Background/Introduction: Sclerostin is a protein that in humans is encoded by the SOST gene. It is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Additionally, Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,974 Da
NCBI Official Full Name
sclerostin
NCBI Official Synonym Full Names
sclerostin
NCBI Official Symbol
Sost
NCBI Protein Information
sclerostin
UniProt Protein Name
Sclerostin
Protein Family
UniProt Gene Name
Sost
UniProt Entry Name
SOST_RAT
NCBI Description
may act as a regulator of bone homeostasis [RGD, Feb 2006]
Uniprot Description
SOST: Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular matrix; extracellular space; Golgi apparatus; protein complex
Molecular Function: protein binding; transcription factor binding
Biological Process: negative regulation of BMP signaling pathway; negative regulation of ossification; negative regulation of protein complex assembly; negative regulation of Wnt receptor signaling pathway; ossification; positive regulation of transcription, DNA-dependent; response to mechanical stimulus
Research Articles on SOST
Similar Products
Product Notes
The Rat SOST sost (Catalog #AAA8248486) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8248486 ELISA Kit recognizes Rat SOST. It is sometimes possible for the material contained within the vial of "Sclerostin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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