Mouse Flt3 Ligand ELISA Kit | FLT3LG elisa kit

Mouse Flt3 Ligand ELISA Kit

Cat. Num. AAA824939

• Gene Names: Flt3l; Ly72L; Flt3lg
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Flt3 Ligand; Mouse Flt3 Ligand ELISA Kit; Fms-related tyrosine kinase 3 ligand; Flt3 ligand; Flt3L; SL cytokine; FLT3LG elisa kit

• Reactivity: Mouse
• Specificity: The Mouse FGF23 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse FGF23 proteins within the range of 15.6 pg/ml-1000 pg/ml.
• Sequence Length: 232

• Applicable Applications for FLT3LG elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: 10 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLT3LG elisa kit

Principle of the Assay: The MyBioSource' Mouse FGF23 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse FGF23 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse FGF23 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF23 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse FGF23 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF23 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. It is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 is secreted by Osteocytes in response to elevated Calcitriol. And it acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF23 decreases the reabsorption and increases excretion of phosphate. Also, FGF23 may suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption.

NCBI and Uniprot Product Information

• NCBI GI #: 227430331
• NCBI GeneID: 14256
• NCBI Accession #: NP_038548.3
• NCBI GenBank Nucleotide #: NM_013520.3
• UniProt Accession #: P49772; Q64085
• Molecular Weight: 28,259 Da
• NCBI Official Full Name: fms-related tyrosine kinase 3 ligand
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 3 ligand
• NCBI Official Symbol: Flt3l
• NCBI Official Synonym Symbols: Ly72L; Flt3lg
• NCBI Protein Information: fms-related tyrosine kinase 3 ligand; flt3 ligand
• UniProt Protein Name: Fms-related tyrosine kinase 3 ligand
• Protein Family: Fms-related tyrosine kinase
• UniProt Gene Name: Flt3lg
• UniProt Synonym Gene Names: Flt3l; Flt3 ligand; Flt3L
• UniProt Entry Name: FLT3L_MOUSE

Uniprot Description

FLT3LG: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Membrane protein, integral; Ligand, receptor tyrosine kinase

Cellular Component: extracellular space; cell surface; membrane; plasma membrane; integral to membrane; extracellular region

Molecular Function: protein homodimerization activity; cytokine activity; receptor tyrosine kinase binding

Biological Process: regulation of myeloid dendritic cell activation; positive regulation of natural killer cell differentiation; lymphocyte differentiation; embryonic hemopoiesis; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; positive regulation of myoblast differentiation