Rat Complement Component 5a ELISA Kit | C5a elisa kit

Rat Complement Component 5a ELISA Kit

Cat. Num. AAA760397

• Gene Names: C5AR1; C5A; C5AR; C5R1; CD88
• Reactivity: Rat
• Synonyms: Complement Component 5a; Rat Complement Component 5a ELISA Kit; C5a; C5a elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of C5a. No significant cross-reactivity or interference between C5a and analogues was observed.
• Sequence Length: 350

• Samples: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5-4000pg/ml
• Sensitivity: <37.5pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C5a were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C5a were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C5a elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- C5a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-C5a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the C5a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of C5a can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 29169287
• NCBI GeneID: 728
• Molecular Weight: 39,336 Da
• NCBI Official Full Name: complement component 5a receptor 1
• NCBI Official Synonym Full Names: complement component 5a receptor 1
• NCBI Official Symbol: C5AR1
• NCBI Official Synonym Symbols: C5A; C5AR; C5R1; CD88
• NCBI Protein Information: C5a anaphylatoxin chemotactic receptor 1
• UniProt Protein Name: C5a anaphylatoxin chemotactic receptor 1
• UniProt Gene Name: C5AR1
• UniProt Synonym Gene Names: C5AR; C5R1; C5a-R; C5aR
• UniProt Entry Name: C5AR1_HUMAN

Uniprot Description

C5aR: a family 1 G-protein coupled receptor that stimulates the GTPase activity of Gi2. Receptor for the chemotactic and inflammatory complement factor C5a. Stimulates chemotaxis, granule enzyme release and superoxide anion production. Phosphorylated in response to C5a or after stimulation of cells with phorbol esters. Expressed on monocytes, granulocytes, dendritic cells, astrocytes and microglia.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: apical part of cell; basolateral plasma membrane; cell surface; cytoplasmic membrane-bound vesicle; cytosol; integral to plasma membrane; plasma membrane

Molecular Function: C5a anaphylatoxin receptor activity; complement component C5a binding; complement component C5a receptor activity

Biological Process: activation of MAPK activity; apoptosis; cell proliferation in hindbrain; cellular defense response; chemotaxis; defense response to Gram-positive bacterium; elevation of cytosolic calcium ion concentration; immune response; inflammatory response; mRNA transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; neutrophil chemotaxis; organ regeneration; phospholipase C activation; positive regulation of angiogenesis; positive regulation of epithelial cell proliferation; response to lipopolysaccharide; response to peptidoglycan; sensory perception of chemical stimulus; signal transduction