Rabbit anti-Human KEAP1 Polyclonal Antibody | anti-KEAP1 antibody

KEAP1, CT (KEAP1, INRF2, KIAA0132, KLHL19, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, Kelch-like protein 19)

Cat. Num. AAA647475

• Gene Names: KEAP1; INrf2; KLHL19
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: KEAP1; Polyclonal Antibody; CT (KEAP1; INRF2; KIAA0132; KLHL19; Kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2; Kelch-like protein 19); Anti -KEAP1; anti-KEAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-KEAP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: KEAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-457 amino acids from the C-terminal region of human KEAP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KEAP1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the KEAP1 antibody detected the KEAP1 protein (arrow).)

Immunohistochemistry (IHC)

(KEAP1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KEAP1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-KEAP1 antibody

Antibodies; Abs to KIAA; orf

NCBI and Uniprot Product Information

• NCBI GI #: 45269145
• NCBI GeneID: 9817
• NCBI Accession #: NP_987096.1
• NCBI GenBank Nucleotide #: NM_203500.1
• UniProt Accession #: Q14145; Q6LEP0; Q8WTX1; Q9BPY9; B3KPD5
• Molecular Weight: 69,666 Da
• NCBI Official Full Name: kelch-like ECH-associated protein 1
• NCBI Official Synonym Full Names: kelch-like ECH-associated protein 1
• NCBI Official Symbol: KEAP1
• NCBI Official Synonym Symbols: INrf2; KLHL19
• NCBI Protein Information: kelch-like ECH-associated protein 1; kelch-like protein 19; cytosolic inhibitor of Nrf2; kelch-like family member 19
• UniProt Protein Name: Kelch-like ECH-associated protein 1
• Protein Family: Kelch-like ECH-associated protein
• UniProt Gene Name: KEAP1
• UniProt Synonym Gene Names: INRF2; KIAA0132; KLHL19; INrf2
• UniProt Entry Name: KEAP1_HUMAN

NCBI Description

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome. Ref.7 Ref.8 Ref.9 Ref.10 Ref.13

Enzyme regulation: Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane. Sulforaphane also inhibits ubiquitination of NFE2L2/NRF2. Ref.10 Ref.13

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Homodimer. Forms a ternary complex with NFE2L2 and PGAM5. Interacts with the N-terminal regulatory domain of NFE2L2/NRF2. Interacts with BPTF and PTMA. Interacts with CUL3. Part of a complex that contains KEAP1, CUL3 and RBX1. Interacts with NFE2L1. Interacts indirectly with ENC1. Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.19

Subcellular location: Cytoplasm. Nucleus. Note: Shuttles between cytoplasm and nucleus. Ref.7 Ref.8 Ref.11 Ref.15

Tissue specificity: Broadly expressed, with highest levels in skeletal muscle. Ref.8

Domain: The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5. Ref.13

Post-translational modification: Ubiquitinated by the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and is subject to proteasomal-independent degradation. Quinone-induced oxidative stress, but not sulforaphane, increases its ubiquitination. Ubiquitination and subsequent degradation is most pronounced following prolonged exposure of cells to oxidative stress, particularly in glutathione-deficient cells that are highly susceptible to oxidative stress. Ref.9 Ref.10

Sequence similarities: Contains 1 BACK (BTB/Kelch associated) domain.Contains 1 BTB (POZ) domain.Contains 6 Kelch repeats.

Sequence caution: The sequence BAA09481.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.