Rat IFN gamma ELISA Kit | IFNG elisa kit

Rat IFN gamma ELISA Kit

Cat. Num. AAA824582

• Gene Names: Ifng; IFNG2
• Reactivity: Rat
• Applications: ELISA
• Synonyms: IFN gamma; Rat IFN gamma ELISA Kit; Interferon gamma; IFN-gamma; Immune interferon; IFNG elisa kit

• Reactivity: Rat
• Specificity: The Rat CXCL5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat CXCL5 proteins within the range of 15.6 pg/ml-1000 pg/ml.
• Sequence Length: 156

• Applicable Applications for IFNG elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernates, Serum, Plasma, Tissue Homogenates
• Sensitivity: 1 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IFNG elisa kit

Principle of the Assay: MBS Rat CXCL5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CXCL5 in Cell Culture Supernates, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Rat CXCL5 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CXCL5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78(ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

NCBI and Uniprot Product Information

• NCBI GI #: 20302055
• NCBI GeneID: 25712
• NCBI Accession #: NP_620235.1
• NCBI GenBank Nucleotide #: NM_138880.2
• UniProt Accession #: P01581
• Molecular Weight: 17,918 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon gamma
• NCBI Official Symbol: Ifng
• NCBI Official Synonym Symbols: IFNG2
• NCBI Protein Information: interferon gamma; IFN-gamma
• UniProt Protein Name: Interferon gamma
• Protein Family: Interferon
• UniProt Gene Name: Ifng
• UniProt Synonym Gene Names: IFN-gamma
• UniProt Entry Name: IFNG_RAT

NCBI Description

an immune molecule produced by T lymphocytes in response to mitogens or antigens [RGD, Feb 2006]

Uniprot Description

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.