Western Blot (WB)
(Western blot analysis of lysates from K562 cell line,mouse stomach tissue lysate(from left to right), using BLMH Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Rabbit BLMH Polyclonal Antibody | anti-BLMH antibody
BLMH Antibody (Center)
Predicted: Rabbit, Rat
Predicted: Rabbit, Rat
IF: 1:25
Western Blot (WB)
(Western blot analysis of lysates from K562 cell line,mouse stomach tissue lysate(from left to right), using BLMH Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of lysates from K562 cell line,mouse stomach tissue lysate(from left to right), using BLMH Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with BLMH Antibody (Center) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with BLMH Antibody (Center) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Western Blot (WB)
(BLMH Antibody (Center) western blot analysis in CEM,HL-60 cell line lysates (35ug/lane).This demonstrates the BLMH antibody detected the BLMH protein (arrow).)
Western Blot (WB)
(BLMH Antibody (Center) western blot analysis in CEM,HL-60 cell line lysates (35ug/lane).This demonstrates the BLMH antibody detected the BLMH protein (arrow).)
Background: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity (By similarity).
NCBI and Uniprot Product Information
NCBI Description
Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq, Jul 2008]
Uniprot Description
BLMH: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. Belongs to the peptidase C1 family.
Protein type: EC 3.4.22.40; Protease
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus
Molecular Function: protein binding; carboxypeptidase activity; cysteine-type endopeptidase activity; aminopeptidase activity; cysteine-type peptidase activity
Biological Process: response to drug; antigen processing and presentation of peptide antigen via MHC class I; protein polyubiquitination; response to toxin; proteolysis
Disease: Alzheimer Disease
Research Articles on BLMH
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Product Notes
The BLMH blmh (Catalog #AAA9231778) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BLMH Antibody (Center) reacts with Human, Mouse Predicted: Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BLMH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000 IF: 1:25. Researchers should empirically determine the suitability of the BLMH blmh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BLMH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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