Western Blot (WB)
(All lanes : Anti-Smarcc1 Antibody (C-term) at 1:1000 dilutionLane 1: C2C12 whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 123 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit Smarcc1 Polyclonal Antibody | anti-Smarcc1 antibody
(Mouse) Smarcc1 Antibody (C-term)
Gene Names
Smarcc1; Rsc8; SRG3; msp3; BAF155; AI115498
Reactivity
Human, MousePredicted: Rat
Applications
Western Blot, Immunofluorescence, ELISA
Synonyms
Smarcc1; Polyclonal Antibody; (Mouse) Smarcc1 Antibody (C-term); SWI/SNF complex subunit SMARCC1; BRG1-associated factor 155; SWI/SNF complex 155 kDa subunit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1; SWI3-related protein; BAF155; Baf155; Srg3; anti-Smarcc1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted: Rat
Predicted: Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This (Mouse) Smarcc1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 963-997 amino acids from the C-terminal region of human (Mouse) Smarcc1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
1104
Applicable Applications for anti-Smarcc1 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA( EIA)
Application Notes
WB: 1:1000
IF: 1:25
IF: 1:25
Antigen Source
Human
Antigen Region
963-997
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-Smarcc1 Antibody (C-term) at 1:1000 dilutionLane 1: C2C12 whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 123 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Smarcc1 Antibody (C-term) at 1:1000 dilutionLane 1: C2C12 whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 123 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with (Mouse) Smarcc1 Antibody (C-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with (Mouse) Smarcc1 Antibody (C-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-Smarcc1 antibody
Description: Purified Rabbit Polyclonal Antibody (Pab)
Background: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Background: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
References
Jeon S.H.,et al.J. Exp. Med. 185:1827-1836(1997). Kim J.K.,et al.Mol. Cell. Biol. 21:7787-7795(2001). Lessard J.,et al.Neuron 55:201-215(2007). Sweet S.M.,et al.Mol. Cell. Proteomics 8:904-912(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
122890
NCBI Official Full Name
SWI/SNF complex subunit SMARCC1
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
NCBI Official Symbol
Smarcc1
NCBI Official Synonym Symbols
Rsc8; SRG3; msp3; BAF155; AI115498
NCBI Protein Information
SWI/SNF complex subunit SMARCC1
UniProt Protein Name
SWI/SNF complex subunit SMARCC1
Protein Family
UniProt Gene Name
Smarcc1
UniProt Synonym Gene Names
Baf155; Srg3; BAF155
UniProt Entry Name
SMRC1_MOUSE
Research Articles on Smarcc1
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Product Notes
The Smarcc1 smarcc1 (Catalog #AAA9231978) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The (Mouse) Smarcc1 Antibody (C-term) reacts with Human, Mouse Predicted: Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Smarcc1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA( EIA). WB: 1:1000 IF: 1:25. Researchers should empirically determine the suitability of the Smarcc1 smarcc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smarcc1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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