Western Blot (WB)
(Western blot analysis of lysates from A431, Hela, HepG2 cell line, mouse brain tissue and MCF-7, U-251 MG cell line (from left to right), using CYC1 Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Rabbit anti-Human, Mouse CYC1 Polyclonal Antibody | anti-CYC1 antibody
CYC1 Antibody (Center)
IHC-P: 1:25
IF: 1:25
Western Blot (WB)
(Western blot analysis of lysates from A431, Hela, HepG2 cell line, mouse brain tissue and MCF-7, U-251 MG cell line (from left to right), using CYC1 Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of lysates from A431, Hela, HepG2 cell line, mouse brain tissue and MCF-7, U-251 MG cell line (from left to right), using CYC1 Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded M. stomach section using CYC1 Antibody (Center) . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded M. stomach section using CYC1 Antibody (Center) . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded H. stomach section using CYC1 Antibody (Center)(C-term) . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded H. stomach section using CYC1 Antibody (Center)(C-term) . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with CYC1 Antibody (Center) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with CYC1 Antibody (Center) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded R. stomach section using CYC1 Antibody (Center) . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded R. stomach section using CYC1 Antibody (Center) . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Background: This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a subunit of the cytochrome bc1 complex, which plays an important role in the mitochondrial respiratory chain by transferring electrons from the Rieske iron-sulfur protein to cytochrome c. Mutations in this gene may cause mitochondrial complex III deficiency, nuclear type 6. [provided by RefSeq, Dec 2013]
Uniprot Description
CYC1: This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain. Belongs to the cytochrome c family.
Protein type: Mitochondrial; Energy Metabolism - oxidative phosphorylation; Membrane protein, integral; Oxidoreductase
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: membrane; mitochondrion; mitochondrial inner membrane; integral to membrane; nucleus
Molecular Function: electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; iron ion binding; heme binding
Biological Process: cellular metabolic process; response to glucagon stimulus
Disease: Mitochondrial Complex Iii Deficiency, Nuclear Type 6
Research Articles on CYC1
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Product Notes
The CYC1 cyc1 (Catalog #AAA9231622) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CYC1 Antibody (Center) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CYC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA). WB: 1:1000 IHC-P: 1:25 IF: 1:25. Researchers should empirically determine the suitability of the CYC1 cyc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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