Western Blot (WB)
(Western blot analysis of lysates from HT1080,ZR-75-1 cell line (from left to right), using CRTAP Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Rabbit CRTAP Polyclonal Antibody | anti-CRTAP antibody
CRTAP Antibody (N-term)
Predicted: Mouse
Predicted: Mouse
IF: 1:25
Western Blot (WB)
(Western blot analysis of lysates from HT1080,ZR-75-1 cell line (from left to right), using CRTAP Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of lysates from HT1080,ZR-75-1 cell line (from left to right), using CRTAP Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with CRTAP Antibody (N-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with CRTAP Antibody (N-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Background: The protein encoded by this gene is similar to the chicken and mouse CRTAP genes. The encoded protein is a scaffolding protein that may influence the activity of at least one member of the cytohesin/ARNO family in response to specific cellular stimuli. Defects in this gene are associated with osteogenesis imperfecta, a connective tissue disorder characterized by bone fragility and low bone mass.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is similar to the chicken and mouse CRTAP genes. The encoded protein is a scaffolding protein that may influence the activity of at least one member of the cytohesin/ARNO family in response to specific cellular stimuli. Defects in this gene are associated with osteogenesis imperfecta, a connective tissue disorder characterized by bone fragility and low bone mass. [provided by RefSeq, Jul 2008]
Uniprot Description
CRTAP: Necessary for efficient 3-hydroxylation of fibrillar collagen prolyl residues. Defects in CRTAP are the cause of osteogenesis imperfecta type 7 (OI7). A connective tissue disorder characterized by short stature, short humeri and femora, coxa vara, white sclera, and the absence of dentinogenesis imperfecta. Multiple fractures are present at birth, and patients manifest moderate-severe bone fragility. Death may occurr in the perinatal period due to secondary respiratory insufficiency. Belongs to the leprecan family.
Protein type: Extracellular matrix; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 3p22.3
Cellular Component: proteinaceous extracellular matrix; extracellular space; endoplasmic reticulum lumen; endoplasmic reticulum
Molecular Function: protein complex binding
Biological Process: extracellular matrix organization and biogenesis; protein stabilization; spermatogenesis; peptidyl-proline hydroxylation to 3-hydroxy-L-proline
Disease: Osteogenesis Imperfecta, Type Vii
Research Articles on CRTAP
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Product Notes
The CRTAP crtap (Catalog #AAA9231673) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CRTAP Antibody (N-term) reacts with Human Predicted: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CRTAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA( EIA). WB: 1:1000 IF: 1:25. Researchers should empirically determine the suitability of the CRTAP crtap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CRTAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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