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10000 results for " Antibodies to Goat Proteins" - showing 3850-3900
Western Blot (WB)
(Western blot analysis of Drosha expression in 293 cell lysate (MBS179115).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DROSHA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DROSHA )
Drosha, Monoclonal Antibody (Cat# AAA179115)
Full Name
Anti-Drosha Rabbit Monoclonal Antibody
Gene Names
DROSHA; RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~21 kDa RAC1 protein using Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T . Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa.)
RAC1, Polyclonal Antibody (Cat# AAA8001072)
Full Name
RAC1 Antibody: PerCP
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse liver, using Phospho-Histone H3 (S10) Antibody(MBS1750293)HIST1H3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H3A Antibody (MBS1750293)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Histone H3, Monoclonal Antibody (Cat# AAA1750293)
Full Name
Anti-Phospho-Histone H3 (S10) Rabbit Monoclonal Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$310/0.1 mg
Dot Blot (DB)
(Dot Blot analysis using Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody (SPC-720). Tissue: DYKDDDDK (DYKDDDDK) Positive Control Lysate. Primary Antibody: Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody (SPC-720) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.)
DYKDDDDK Tag, Polyclonal Antibody (Cat# AAA8000392)
Full Name
DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-DYKDDDDK M2 Antibody): ATTO 390
Reactivity
Species Independent
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Dot Blot (DB)
(Dot Blot analysis using Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody (SPC-720). Tissue: DYKDDDDK (DYKDDDDK) Positive Control Lysate. Primary Antibody: Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody (SPC-720) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.)
DYKDDDDK Tag, Polyclonal Antibody (Cat# AAA8000407)
Full Name
DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-DYKDDDDK M2 Antibody): RPE
Reactivity
Species Independent
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2D6. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
KDEL, Monoclonal Antibody (Cat# AAA809097)
Full Name
KDEL Antibody, Clone 2D6: ATTO 390
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(All lanes : Anti-TXN at 1:2000 dilutionLane 1: SH-SY5Y whole cell lysateLane 2: A431 whole cell lysateLane 3: Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 12 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
TXN, Monoclonal Antibody (Cat# AAA9231280)
Full Name
TXN Antibody
Gene Names
TXN; TRX; TRDX; TRX1
Reactivity
Human
Applications
WB, EIA
Purity
Purified through a protein G column, followed by dialysis against PBS.
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Sulfotyrosine Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Sulfotyrosine, Monoclonal Antibody (Cat# AAA808907)
Full Name
Sulfotyrosine Antibody, Clone 7C5: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~21.6 kDa KRAS protein using Rabbit Anti-KRAS Polyclonal Antibody (SPC-777). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-KRAS Polyclonal Antibody (SPC-777) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.6 kDa. Other Band(s): ~29 kDa (PTM).)
KRAS, Polyclonal Antibody (Cat# AAA8001430)
Full Name
KRAS Antibody: HRP
Gene Names
KRAS; NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ERp57 Monoclonal Antibody, Clone 4F9 . Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ERp57 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic reticulum, Melanosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ERp57 Antibody (D) Composite.)
ERp57, Monoclonal Antibody (Cat# AAA808442)
Full Name
ERp57 Antibody, Clone 4F9: FITC
Gene Names
PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
Reactivity
Human
Applications
WB, ICC, IF
Purity
Protein G Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Transferrin expression in Human serum lysate (MBS179096).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TF monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TF )
Transferrin, Monoclonal Antibody (Cat# AAA179096)
Full Name
Anti-Transferrin Rabbit Monoclonal Antibody
Gene Names
TF; TFQTL1; PRO1557; PRO2086; HEL-S-71p
Reactivity
Human, Mouse, Rat
Applications
IP, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western Blot analysis in mouse cerebellum, brain tissue lysates (35ug/lane) usingMBS6004735. VGLU2 (arrow) was detected usingMBS6004735.)
VGLU2, Polyclonal Antibody (Cat# AAA6362574)
Full Name
VGLU2, CT (Slc17a6, Dnpi, Vglut2, Vesicular glutamate transporter 2, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6) (MaxLight 750)
Gene Names
Slc17a6; DNPI; VGLUT2; 2900073D12Rik
Reactivity
Mouse, Human
Applications
IHC, WB
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$880/0.1 mL | $3,945/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~21 kDa RAC1 protein using Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T . Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa.)
RAC1, Polyclonal Antibody (Cat# AAA8001061)
Full Name
RAC1 Antibody: ATTO 594
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of PRDX1 expression in K562 cell lysates (MBS179750).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRDX1 )
PRDX1/Peroxiredoxin 1, Monoclonal Antibody (Cat# AAA179750)
Full Name
Anti-PRDX1/Peroxiredoxin 1 Rabbit Monoclonal Antibody
Gene Names
PRDX1; PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A
Reactivity
Human, Mouse
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Elk1 expression in (1)Neuro-2a cell lysates; (2)K562 cell lysate (MBS179653).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ELK1 )
ELK1, Monoclonal Antibody (Cat# AAA179653)
Full Name
Anti-ELK1 Rabbit Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody (SPC-743). Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody (SPC-743) at 1:60 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) HSP70 Antibody (Acetyl Lys77). (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
HSP70, Polyclonal Antibody (Cat# AAA8000814)
Full Name
HSP70 Antibody (Acetyl Lys77): Alkaline Phosphatase
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Reactivity
Human; Mouse; Rat
Applications
WB, EIA, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$550/0.1 mL | $2,245/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Histone H3 (mono methyl R17) expression in HeLa cell lysate (MBS1750130).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIST1H3A )
Histone H3, Monoclonal Antibody (Cat# AAA1750130)
Full Name
Anti-Histone H3 (mono methyl R17) Rabbit Monoclonal Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(All lanes : Anti-NR3C1 at dilutionLane 1: DU145 whole cell lysateLane 2: U-251MG whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NR3C1, Monoclonal Antibody (Cat# AAA9232297)
Full Name
NR3C1
Gene Names
NR3C1; GR; GCR; GRL; GCCR
Reactivity
Human, Mouse
Predicted : Human , Mouse
Predicted : Human , Mouse
Applications
WB, EIA
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Western Blot (WB)
(Western blot analysis of RGS1 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). RGS1 (arrow) was detected using the purified Pab.)
RGS1, Polyclonal Antibody (Cat# AAA6497465)
Full Name
RGS1, NT (B Cell Activation Protein BL34, Early Response Protein 1R20, Regulator of G-protein Signaling 1, 1R20, BL34, IER1) (AP)
Gene Names
RGS1; 1R20; BL34; IER1; IR20
Reactivity
Human
Applications
WB, EIA
Purity
Purified by Immunoaffinity chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 1F2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
KDEL, Monoclonal Antibody (Cat# AAA809075)
Full Name
KDEL Antibody, Clone 1F2: PerCP
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2D6. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
KDEL, Monoclonal Antibody (Cat# AAA809096)
Full Name
KDEL Antibody, Clone 2D6
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 2D3.1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Citrulline, Monoclonal Antibody (Cat# AAA808513)
Full Name
Citrulline Antibody, Clone 2D3.1: Biotin
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,255/5x0.1 mg
Testing Data
(Y1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SF-1 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SF-1 (~53kD).)
Steroidogenic Factor 1, Polyclonal Antibody (Cat# AAA6501149)
Full Name
Steroidogenic Factor 1 (SF-1, NR5A1) (MaxLight 405)
Gene Names
Nr5a1; ELP; SF1; SF-1; Ad4BP; ELP-3; Ftzf1; Ftz-F1
Reactivity
Mouse
Applications
IB, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$875/0.1 mL | $3,920/5x0.1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Citrulline, Monoclonal Antibody (Cat# AAA808533)
Full Name
Citrulline Antibody, Clone 6C2.1: HRP
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$545/0.1 mg | $2,215/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 7D4. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Dityrosine Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Dityrosine, Monoclonal Antibody (Cat# AAA808871)
Full Name
Dityrosine Antibody, Clone 7D4: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 94 kDa GRP94 protein using Mouse Anti-GRP94 Monoclonal Antibody, Clone 6B12. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-GRP94 Monoclonal Antibody at 1:1000 for O/N at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 94 kDa.)
GRP94, Monoclonal Antibody (Cat# AAA809129)
Full Name
GRP94 Antibody, Clone 6B12: PerCP
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB)
(All lanes : Anti-TAP2 Antibody (N-Term) at 1:2000 dilutionLane 1: Human placenta lysateLane 2: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 76 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
TAP2, Polyclonal Antibody (Cat# AAA9231245)
Full Name
TAP2 Antibody (N-Term)
Gene Names
TAP2; APT2; PSF2; ABC18; ABCB3; PSF-2; RING11; D6S217E
Reactivity
Human
Applications
WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Immunohistochemistry (IHC)
(Figure 1. IHC analysis of SOD3 using anti-SOD3 antibody (MBS1750716).SOD3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SOD3 Antibody (MBS1750716) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
SOD3/Ec Sod, Polyclonal Antibody (Cat# AAA1750716)
Full Name
Anti-SOD3/Ec Sod Picoband antibody
Gene Names
SOD3; EC-SOD
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of ~21.3 kDa HRAS protein using Rabbit Anti-HRAS Polyclonal Antibody (SPC-776). Lane 1: Molecular Weight Ladder (MW). Lane 2: A549. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HRAS Polyclonal Antibody (SPC-776) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.3 kDa. Other Band(s): 25 kDa.)
HRAS, Polyclonal Antibody (Cat# AAA8001414)
Full Name
HRAS Antibody: PerCP
Gene Names
HRAS; CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ERp57 Monoclonal Antibody, Clone 4F9 . Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ERp57 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic reticulum, Melanosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ERp57 Antibody (D) Composite.)
ERp57, Monoclonal Antibody (Cat# AAA808445)
Full Name
ERp57 Antibody, Clone 4F9: PerCP
Gene Names
PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
Reactivity
Human
Applications
WB, ICC, IF
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 7E1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
7-Ketocholesterol, Monoclonal Antibody (Cat# AAA808719)
Full Name
7-Ketocholesterol Antibody, Clone 7E1: ATTO 390
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,280/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~21 kDa RAC1 protein using Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T . Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa.)
RAC1, Polyclonal Antibody (Cat# AAA8001069)
Full Name
RAC1 Antibody: FITC
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6009410. This demonstrates that MBS6009410 detected the SLC16A10 protein (arrow).)
SLC16A10, Polyclonal Antibody (Cat# AAA6348079)
Full Name
SLC16A10, CT (SLC16A10, MCT10, TAT1, Monocarboxylate transporter 10, Aromatic amino acid transporter 1, Solute carrier family 16 member 10, T-type amino acid transporter 1) (Biotin)
Gene Names
SLC16A10; TAT1; MCT10; PRO0813
Reactivity
Human
Applications
IHC, WB, FC/FACS, EIA
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22349a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22349a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
SHC2, Polyclonal Antibody (Cat# AAA9231297)
Full Name
SHC2 Antibody (N-Term)
Gene Names
SHC2; SCK; SLI; SHCB
Reactivity
Human
Predicted: Human
Predicted: Human
Applications
IF, FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Histone H3 (di methyl K9) expression in HeLa cell lysate (MBS1750128).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIST1H3A )
Histone H3, Monoclonal Antibody (Cat# AAA1750128)
Full Name
Anti-Histone H3 (di methyl K9) Rabbit Monoclonal Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
ChIP, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22336a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22336a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
GCA, Polyclonal Antibody (Cat# AAA9231283)
Full Name
GCA Antibody (N-Term)
Gene Names
GCA; GCL
Reactivity
Human
Applications
IF, FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Histone H4 (tri methyl K20) expression in HeLa cell lysate (MBS1750138).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H4A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIST1H4A )
Histone H4, Monoclonal Antibody (Cat# AAA1750138)
Full Name
Anti-Histone H4 (tri methyl K20) Rabbit Monoclonal Antibody
Gene Names
HIST2H4B; H4/o
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Testing Data
(10ug acid-extracted HeLa cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS603404 (0.01ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Arrow indicates HP1g (25kD).)
Heterochromatin Protein 1, gamma, Monoclonal Antibody (Cat# AAA6244017)
Full Name
Heterochromatin Protein 1, gamma (HP1g, M32) (HRP)
Gene Names
CBX3; HECH; HP1-GAMMA; HP1Hs-gamma
Reactivity
Human, Mouse
Applications
IHC, IP, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$1,095/0.1 mL | $4,925/5x0.1 mL
Western Blot (WB)
(Western Blot analysis: Bovine cardiac preparation, treated with PKA (lane 1) or untreated (lane 2), was probed with MBS617405 (0.5ug/ml).)
Phospholamban, Polyclonal Antibody (Cat# AAA6493254)
Full Name
Phospholamban, Phosphorylated (Ser16) (AP)
Gene Names
PLN; PLB; CMD1P; CMH18
Reactivity
Human, Bovine
Applications
WB
Purity
Purified by Immunoaffinity chromatography.
Pricing
$1,055/0.1 mL | $4,735/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
KDEL, Monoclonal Antibody (Cat# AAA809078)
Full Name
KDEL Antibody, Clone 2C1
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 1F2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
KDEL, Monoclonal Antibody (Cat# AAA809060)
Full Name
KDEL Antibody, Clone 1F2
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
KDEL, Monoclonal Antibody (Cat# AAA809079)
Full Name
KDEL Antibody, Clone 2C1: ATTO 390
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
KDEL, Monoclonal Antibody (Cat# AAA809091)
Full Name
KDEL Antibody, Clone 2C1: HRP
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2D6. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
KDEL, Monoclonal Antibody (Cat# AAA809107)
Full Name
KDEL Antibody, Clone 2D6: Biotin
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western Blot analysis in mouse cerebellum tissue lysates (35ug/lane) usingMBS643103. FMOD (arrow) was detected usingMBS643103.)
FMOD, Polyclonal Antibody (Cat# AAA6299488)
Full Name
FMOD, CT (FMOD, FM, SLRR2E, Fibromodulin, Collagen-binding 59kD protein, Keratan sulfate proteoglycan fibromodulin) (PE)
Gene Names
FMOD; FM; SLRR2E
Reactivity
Human, Mouse
Applications
IHC, WB, FC/FACS
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Testing Data
(NG108-15 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SETA, (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SETA (85kD).)
SETA, Monoclonal Antibody (Cat# AAA6245627)
Full Name
SETA (CIN85, Ruk, SH3KBPI) (MaxLight 490)
Gene Names
SH3KBP1; HSB1; CIN85; GIG10; HSB-1; MIG18; CD2BP3
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$905/0.1 mL | $4,055/5x0.1 mL
Western Blot (WB)
(CT45A Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CT45A antibody detected the CT45A protein (arrow).)
CT45A, Polyclonal Antibody (Cat# AAA6289595)
Full Name
CT45A, NT (CT45-4,CT45A4,CT45-1,CT45A1,CT45-2,CT45A2,CT45-3,CT45A3,CT45-5,CT45A5,CT45-6,CT45A6) (APC)
Gene Names
CT45A1; CT45; CT45-1; CT45.1
Reactivity
Human
Applications
FC/FACS, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
KDEL, Monoclonal Antibody (Cat# AAA809094)
Full Name
KDEL Antibody, Clone 2C1: RPE
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Citrulline, Monoclonal Antibody (Cat# AAA808535)
Full Name
Citrulline Antibody, Clone 6C2.1: PerCP
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,270/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Citrulline, Monoclonal Antibody (Cat# AAA808529)
Full Name
Citrulline Antibody, Clone 6C2.1: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
