Rabbit anti-Human, Mouse Fyn Polyclonal Antibody | anti-Fyn antibody

Fyn, NT (FYN Oncogene Related to SRC FGR YES, MGC45350, p59-Fyn, Proto-oncogene c-Fyn, Proto-oncogene Syn, SLK, SYN, Tyrosine Protein Kinase Fyn) (Biotin)

Cat. Num. AAA6477218

• Gene Names: FYN; SLK; SYN; p59-FYN
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Fyn; Polyclonal Antibody; NT (FYN Oncogene Related to SRC FGR YES; MGC45350; p59-Fyn; Proto-oncogene c-Fyn; Proto-oncogene Syn; SLK; SYN; Tyrosine Protein Kinase Fyn) (Biotin); EC=2.7.10.2; Protein Tyrosine Kinase Fyn; Proto-oncogene Tyrosine Protein Kinase Fyn; Src Yes Related Novel Gene; Src/yes Related Novel Gene; anti-Fyn antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FYN. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-Fyn antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human FYN (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of FYN (arrow) using rabbit polyclonal FYN Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FYN gene.)

Western Blot (WB)

(Western blot analysis of anti-FYN (N-term) Pab in mouse liver tissue lysate. FYN (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Flow Cytometry (FC/FACS)

(FYN Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-Fyn antibody

Antibodies; Tyrosine Kinases

References

1. Taniguchi, S., et al., Biochem. Biophys. Res. Commun. 306(1):151-155 (2003). 2. Chan, B., et al., Nat. Cell Biol. 5(2):155-160 (2003). 3. Goldsmith, J.F., et al., Biochem. Biophys. Res. Commun. 298(4):501-504 (2002). 4. Freund, C., et al., EMBO J. 21(22):5985-5995 (2002). 5. Parravicini, V., et al., Nat. Immunol. 3(8):741-748 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4503823
• NCBI GeneID: 2534
• NCBI Accession #: NP_002028.1
• NCBI GenBank Nucleotide #: NM_002037.5
• UniProt Accession #: P06241; Q16248; Q5R3A6; Q5R3A7; Q8N5D7; B5BU57; E1P557; H0UI48
• Molecular Weight: 60,762 Da
• NCBI Official Full Name: tyrosine-protein kinase Fyn isoform a
• NCBI Official Synonym Full Names: FYN oncogene related to SRC, FGR, YES
• NCBI Official Symbol: FYN
• NCBI Official Synonym Symbols: SLK; SYN; p59-FYN
• NCBI Protein Information: tyrosine-protein kinase Fyn; src-like kinase; proto-oncogene Syn; c-syn protooncogene; proto-oncogene c-Fyn; src/yes-related novel; tyrosine kinase p59fyn(T); OKT3-induced calcium influx regulator
• UniProt Protein Name: Tyrosine-protein kinase Fyn
• Protein Family: FYN-binding protein
• UniProt Gene Name: FYN
• UniProt Synonym Gene Names: SLK
• UniProt Entry Name: FYN_HUMAN

NCBI Description

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]

Uniprot Description

Fyn: a tyrosine kinase of the Src family. Implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Three alternatively spliced isoforms have been described. Isoform 2 shows a greater ability to mobilize cytoplasmic calcium than isoform 1. Induced expression aids in cellular transformation and xenograft metastasis. In squamous cell carcinoma, Fyn transduces signals from EGFR and Src and is required for cell migration and invasiveness. Activity linked to migration in a murine melanoma model. Appears to block late stage development of neuroblastoma. Mouse knockout deficient in kindling response, a model for human epilepsy.

Protein type: Protein kinase, TK; Oncoprotein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Kinase, protein; TK group; Src family

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: extrinsic to internal side of plasma membrane; mitochondrion; postsynaptic density; plasma membrane; actin filament; cytosol; nucleus; endosome; lipid raft

Molecular Function: tubulin binding; CD8 receptor binding; ephrin receptor binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; G-protein-coupled receptor binding; peptide hormone receptor binding; protein-tyrosine kinase activity; T cell receptor binding; phosphoinositide 3-kinase binding; glycoprotein binding; CD4 receptor binding; ATP binding

Biological Process: axon guidance; central nervous system development; viral reproduction; T cell activation; nerve growth factor receptor signaling pathway; neuron migration; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of apoptosis; regulation of cell shape; calcium ion transport; forebrain development; ephrin receptor signaling pathway; feeding behavior; negative regulation of neuron apoptosis; cell differentiation; epidermal growth factor receptor signaling pathway; response to drug; platelet activation; cell migration; fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; dendrite morphogenesis; activated T cell proliferation; learning; regulation of defense response to virus by virus; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; detection of mechanical stimulus involved in sensory perception of pain; response to ethanol; T cell costimulation; innate immune response; negative regulation of protein catabolic process; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway