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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for " Antibodies to Goat Proteins" - showing 3650-3700
Western Blot (WB)
(Figure 1. Western blot analysis of TFF2 using anti-TFF2 antibody (MBS1750917).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat gaster tissue lysates,Lane 2: mouse gaster tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFF2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TFF2 at approximately 14KD. The expected band size for TFF2 is at 14KD. )
TFF2, Polyclonal Antibody (Cat# AAA1750917)
Full Name
Anti-TFF2 Picoband Antibody
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
7-Ketocholesterol, Monoclonal Antibody (Cat# AAA808737)
Full Name
7-Ketocholesterol Antibody, Clone 3F7: ATTO 390
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,280/5x0.1 mg
Western Blot (WB)
(All lanes : Anti-STK25 Antibody at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Human testis lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
STK25, Polyclonal Antibody (Cat# AAA9231096)
Full Name
STK25 Antibody
Gene Names
STK25; SOK1; YSK1
Reactivity
Human
Applications
WB, EIA
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Anti-PKN1 Antibody (C-Term) at 1:2000 dilution + Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 104 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
PKN1, Polyclonal Antibody (Cat# AAA9231043)
Full Name
PKN1 Antibody (C-Term)
Gene Names
PKN1; DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
Reactivity
Human
Predicted: Bovine, Drosophila
Predicted: Bovine, Drosophila
Applications
WB, EIA
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil, using FLI1 Antibody (MBS179310)FLI1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FLI1 Antibody (MBS179310)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
FLI1, Monoclonal Antibody (Cat# AAA179310)
Full Name
Anti-FLI1 Rabbit Monoclonal Antibody
Gene Names
FLI1; EWSR2; SIC-1; BDPLT21
Reactivity
Human, Mouse
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rad21 expression in HeLa cell lysate (MBS179755).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD21 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAD21 )
Rad21, Monoclonal Antibody (Cat# AAA179755)
Full Name
Anti-Rad21 Rabbit Monoclonal Antibody
Gene Names
RAD21; HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(IGKV A18 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the IGKVA18 antibody detected the IGKVA18 protein (arrow).)
IGKV A18, Polyclonal Antibody (Cat# AAA6309562)
Full Name
IGKV A18, CT (IGKV A18) (PE)
Reactivity
Human
Applications
WB, FC
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
7-Ketocholesterol, Monoclonal Antibody (Cat# AAA808747)
Full Name
7-Ketocholesterol Antibody, Clone 3F7: Biotin
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,255/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
O-GlcNAc, Monoclonal Antibody (Cat# AAA808550)
Full Name
O-GlcNAc Antibody, Clone 9H6: FITC
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
PEG Purified
Pricing
$550/0.1 mg | $2,235/5x0.1 mg
Western Blot (WB)
(Western blot analysis of SMC3 expression in HeLa cell lysate (MBS179770).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SMC3 )
SMC3, Monoclonal Antibody (Cat# AAA179770)
Full Name
Anti-SMC3 Rabbit Monoclonal Antibody
Gene Names
SMC3; BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-Cdc6 (S54) expression in (1) Raji cell lysate; (2) Raji + FBS cell lysate (MBS1750256).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC6 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC6 )
Cdc6, Monoclonal Antibody (Cat# AAA1750256)
Full Name
Anti-Phospho-Cdc6 (S54) Rabbit Monoclonal Antibody
Gene Names
CDC6; CDC18L; HsCDC6; MGORS5; HsCDC18
Reactivity
Human
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(All lanes : Anti-NSFL1C Antibody (Center) at 1:2000 dilutionLane 1: 293 whole cell lysateLane 2: A431 whole cell lysateLane 3: NCI-H1299 whole cell lysateLane 4: U-251 MG whole cell lysateLane 5: Mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NSFL1C, Polyclonal Antibody (Cat# AAA9231042)
Full Name
NSFL1C Antibody (Center)
Gene Names
NSFL1C; P47; UBX1; UBXD10; UBXN2C; dJ776F14.1
Reactivity
Human, Mouse
Predicted: Bovine, Rat
Predicted: Bovine, Rat
Applications
WB, EIA
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 7E1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
7-Ketocholesterol, Monoclonal Antibody (Cat# AAA808734)
Full Name
7-Ketocholesterol Antibody, Clone 7E1: RPE
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,260/5x0.1 mg
ELISA (EIA)
(Figure 1 ELISA Validation Antibodies: SARS-CoV-2 (COVID-19) Envelope Antibody, 9169. A direct ELISA was performed using immunogen peptide (3531P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Envelope antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.)
COVID 19 Envelope Coronavirus, Polyclonal Antibody (Cat# AAA154667)
Full Name
SARS-CoV-2 (COVID-19) Envelope Antibody
Reactivity
Virus
Applications
EIA
Purity
SARS-CoV-2 (COVID-19) Envelope Antibody is affinity chromatography purified via peptide column
Pricing
$495/0.1 mg | $2,225/5x0.1 mg
Western Blot (WB)
(Anti-MPL Antibody (C-Term) at 1:2000 dilution + Mouse spleen lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
MPL, Polyclonal Antibody (Cat# AAA9231253)
Full Name
MPL Antibody (C-Term)
Gene Names
MPL; MPLV; TPOR; C-MPL; CD110; THCYT2
Reactivity
Mouse
Predicted: Human
Predicted: Human
Applications
WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 2D3.1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Citrulline, Monoclonal Antibody (Cat# AAA808506)
Full Name
Citrulline Antibody, Clone 2D3.1: ATTO 594
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 2D3.1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Citrulline, Monoclonal Antibody (Cat# AAA808511)
Full Name
Citrulline Antibody, Clone 2D3.1: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
O-GalNAc, Monoclonal Antibody (Cat# AAA808572)
Full Name
O-GalNAc Antibody, Clone 9B9: RPE
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
PEG Purified
Pricing
$555/0.1 mg | $2,260/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Histone H4 (mono methyl K16) expression in (1) NIH/3T3 cell lysate; (2) A549 cell lysate (MBS1750139).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H4A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIST1H4A )
Histone H4, Monoclonal Antibody (Cat# AAA1750139)
Full Name
Anti-Histone H4 (mono methyl K16) Rabbit Monoclonal Antibody
Gene Names
HIST2H4B; H4/o
Reactivity
Human, Mouse
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(WB: EGF-stimulated A431 cell lysate probed with MBS609422 (0.5ug/ml).)
14-3-3, sigma, Monoclonal Antibody (Cat# AAA6241568)
Full Name
14-3-3, sigma (SFN, Stratifin, HGNC:10773) (AP)
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$930/0.1 mL | $4,170/5x0.1 mL
Western Blot (WB)
(Western Blot analysis: Lysate from Hela cells was resolved by electrophoresis, transferred to PVDF and probed withMBS607308. Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates delta 1 Catenin (90-120kD).)
Catenin, delta 1, Monoclonal Antibody (Cat# AAA6246709)
Full Name
Catenin, delta 1 (Cadherin-associated Src substrate, CAS, Catenin delta-1, CTNND, KIAA0384, p120, p120(cas), p120(ctn), p120cas, P120CAS, p120 Catenin, p120ctn, P120CTN) (MaxLight 650)
Gene Names
CTNND1; CAS; p120; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)
Reactivity
Human, Mouse
Applications
FC/FACS, ICC, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$885/0.1 mL | $3,980/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
KDEL, Monoclonal Antibody (Cat# AAA809093)
Full Name
KDEL Antibody, Clone 2C1: PerCP
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Citrulline, Monoclonal Antibody (Cat# AAA808520)
Full Name
Citrulline Antibody, Clone 6C2.1
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$505/0.1 mg | $2,050/5x0.1 mg
Western Blot (WB)
(Anti-RARRES2 Antibody (N-Term) at 1:2000 dilution + Human lung lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
RARRES2, Polyclonal Antibody (Cat# AAA9231038)
Full Name
RARRES2 Antibody (N-Term)
Gene Names
RARRES2; TIG2; HP10433
Reactivity
Human
Applications
WB, EIA
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 2D3.1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Citrulline, Monoclonal Antibody (Cat# AAA808514)
Full Name
Citrulline Antibody, Clone 2D3.1: FITC
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,235/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 7D4. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Dityrosine Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Dityrosine, Monoclonal Antibody (Cat# AAA808862)
Full Name
Dityrosine Antibody, Clone 7D4
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$505/0.1 mg | $2,050/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ERp57 Monoclonal Antibody, Clone 4F9 . Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ERp57 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic reticulum, Melanosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ERp57 Antibody (D) Composite.)
ERp57, Monoclonal Antibody (Cat# AAA808430)
Full Name
ERp57 Antibody, Clone 4F9
Gene Names
PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
Reactivity
Human
Applications
WB, ICC, IF
Purity
Protein G Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
O-GlcNAc, Monoclonal Antibody (Cat# AAA808547)
Full Name
O-GlcNAc Antibody, Clone 9H6: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
PEG Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
O-GlcNAc, Monoclonal Antibody (Cat# AAA808540)
Full Name
O-GlcNAc Antibody, Clone 9H6: ATTO 488
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
PEG Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 7E1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
7-Ketocholesterol, Monoclonal Antibody (Cat# AAA808720)
Full Name
7-Ketocholesterol Antibody, Clone 7E1: ATTO 488
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Peroxiredoxin 2 expression in HEK293 cell lysate (MBS179780).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRDX2 )
Peroxiredoxin 2, Monoclonal Antibody (Cat# AAA179780)
Full Name
Anti-Peroxiredoxin 2 Rabbit Monoclonal Antibody
Gene Names
PRDX2; PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B; HEL-S-2a
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western Blot from a previous lot. RIPA lysates of transiently transfected Cos-1 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS612660 (1ug/ml). Proteins were visualized using IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Lane 1: pUSE untransfected control; Lane 2: Transfected cells. Arrow indicates NPT II (30kD).)
Neomycin Phosphotransferase II, Polyclonal Antibody (Cat# AAA6489708)
Full Name
Neomycin Phosphotransferase II (NPT II) (Biotin)
Applications
EIA, WB
Purity
Purified by Protein A Affinity Chromatography
Pricing
$995/0.1 mL | $4,470/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of Hela cell line lysates (35ug/lane) usingMBS629125. FZD1(arrow) was detected usingMBS629125.)
FZD1, Polyclonal Antibody (Cat# AAA6476945)
Full Name
FZD1, ID (Frizzled-1, Fz-1, hFz1, FzE1) (MaxLight 405)
Reactivity
Human
Applications
WB, FC/FACS, IHC
Purity
Purified by Protein A/G Affinity Chromatography.
Pricing
$880/0.1 mL | $3,945/5x0.1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 7D4. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Dityrosine Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Dityrosine, Monoclonal Antibody (Cat# AAA808875)
Full Name
Dityrosine Antibody, Clone 7D4: HRP
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$545/0.1 mg | $2,215/5x0.1 mg
Western Blot (WB)
(Western blot analysis of TBP expression in K562 cell lysate (MBS179251).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBP monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TBP )
TBP/Tata Binding Protein Tbp, Monoclonal Antibody (Cat# AAA179251)
Full Name
Anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal Antibody
Gene Names
TBP; HDL4; GTF2D; SCA17; TFIID; GTF2D1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1 Western Blot Validation with SARS-CoV-2 Nucleocapsid Recombinant Protein Loading: 50 ng per lane of SARS-CoV-2 Nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 Nucleocapsid 9103, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL, Lane 3: 2 μg/mL)
COVID 19 Nucleocapsid (NP) Coronavirus, Polyclonal Antibody (Cat# AAA154654)
Full Name
SARS-CoV-2 (COVID-19) Nucleocapsid Antibody
Reactivity
Virus
Applications
EIA, WB
Purity
Affinity chromatography purified via peptide column.
Pricing
$495/0.1 mg | $2,225/5x0.1 mg
Western Blot (WB)
(Western blot analysis of KMT6 / EZH2 expression in Hela cell lysate (MBS179062).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EZH2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EZH2 )
KMT6/EZH2, Monoclonal Antibody (Cat# AAA179062)
Full Name
Anti-KMT6/EZH2 Rabbit Monoclonal Antibody
Gene Names
EZH2; WVS; ENX1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody (SPC-743). Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody (SPC-743) at 1:60 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) HSP70 Antibody (Acetyl Lys77). (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
HSP70, Polyclonal Antibody (Cat# AAA8000805)
Full Name
HSP70 Antibody (Acetyl Lys77)
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Reactivity
Human; Mouse; Rat
Applications
WB, EIA, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$505/0.1 mL | $2,050/5x0.1 mL
Western Blot (WB)
(Western blot analysis of DLL3 Antibody (C-term) in A375 cell line lysates (35ug/lane). DLL3 (arrow) was detected using the purified Pab.)
DLL3, Polyclonal Antibody (Cat# AAA6292367)
Full Name
DLL3, CT (DLL3, Delta-like protein 3, Drosophila Delta homolog 3) (APC)
Gene Names
DLL3; SCDO1
Reactivity
Human
Applications
IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
KDEL, Monoclonal Antibody (Cat# AAA809089)
Full Name
KDEL Antibody, Clone 2C1: Biotin
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(All lanes : Anti-GBA2 Antibody (Center) at 1:2000 dilutionLane 1: human brain lysateLane 2: mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 105 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
GBA2, Polyclonal Antibody (Cat# AAA9231018)
Full Name
GBA2 Antibody (Center)
Gene Names
GBA2; AD035; SPG46; NLGase
Reactivity
Human, Mouse
Predicted: Rat
Predicted: Rat
Applications
WB, EIA
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Western blot analysis of ANR49 Antibody (Center) in Jurkat cell line lysates (35ug/lane). ANR49 (arrow) was detected using the purified Pab.)
ANR49, Polyclonal Antibody (Cat# AAA6273898)
Full Name
ANR49, ID (ANKRD49, FGIF, Ankyrin repeat domain-containing protein 49, Fetal globin-inducing factor) (FITC)
Gene Names
ANKRD49; FGIF; GBIF
Reactivity
Human
Applications
FC/FACS, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 7D4. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Dityrosine Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Dityrosine, Monoclonal Antibody (Cat# AAA808864)
Full Name
Dityrosine Antibody, Clone 7D4: ATTO 488
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Sulfotyrosine Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Sulfotyrosine, Monoclonal Antibody (Cat# AAA808911)
Full Name
Sulfotyrosine Antibody, Clone 7C5: HRP
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$545/0.1 mg | $2,215/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 94 kDa GRP94 protein using Mouse Anti-GRP94 Monoclonal Antibody, Clone 6B12. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-GRP94 Monoclonal Antibody at 1:1000 for O/N at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 94 kDa.)
GRP94, Monoclonal Antibody (Cat# AAA809125)
Full Name
GRP94 Antibody, Clone 6B12: Biotin
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of ID4 Antibody (N-term) in mouse bladder tissue lysates (35ug/lane). ID4 (arrow) was detected using the purified Pab.)
ID4, Polyclonal Antibody (Cat# AAA6309147)
Full Name
ID4, NT (ID4, BHLHB27, DNA-binding protein inhibitor ID-4, Class B basic helix-loop-helix protein 27, Inhibitor of DNA binding 4) (Biotin)
Gene Names
ID4; IDB4; bHLHb27
Reactivity
Human, Mouse
Applications
FC/FACS, EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ERp57 Monoclonal Antibody, Clone 4F9 . Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ERp57 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic reticulum, Melanosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ERp57 Antibody (D) Composite.)
ERp57, Monoclonal Antibody (Cat# AAA808434)
Full Name
ERp57 Antibody, Clone 4F9: ATTO 594
Gene Names
PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
Reactivity
Human
Applications
WB, ICC, IF
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of GPX1 expression in 293T cell lysate (MBS179526).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPX1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GPX1 )
GPX1/Glutathione Peroxidase 1, Monoclonal Antibody (Cat# AAA179526)
Full Name
Anti-GPX1/Glutathione Peroxidase 1 Rabbit Monoclonal Antibody
Gene Names
GPX1; GPXD; GSHPX1
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~21.6 kDa KRAS protein using Rabbit Anti-KRAS Polyclonal Antibody (SPC-777). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-KRAS Polyclonal Antibody (SPC-777) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.6 kDa. Other Band(s): ~29 kDa (PTM).)
KRAS, Polyclonal Antibody (Cat# AAA8001417)
Full Name
KRAS Antibody
Gene Names
KRAS; NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Cyclophilin A expression in HepG2 cell lysate (MBS179625).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPIA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PPIA )
Cyclophilin A, Monoclonal Antibody (Cat# AAA179625)
Full Name
Anti-Cyclophilin A Rabbit Monoclonal Antibody
Gene Names
PPIA; CYPA; CYPH; HEL-S-69p
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
