Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22349a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22349a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Rabbit SHC2 Polyclonal Antibody | anti-SHC2 antibody
SHC2 Antibody (N-Term)
Gene Names
SHC2; SCK; SLI; SHCB
Reactivity
HumanPredicted: Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Synonyms
SHC2; Polyclonal Antibody; SHC2 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); SHC-transforming protein 2; Protein Sck; SHC-transforming protein B; Src homology 2 domain-containing-transforming protein C2; SH2 domain protein C2; SCK; SHCB; anti-SHC2 antibody
Host
Rabbit
Reactivity
Human
Predicted: Human
Predicted: Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This SHC2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 129-163 amino acids from the human region of human SHC2.
Purity/Purification
Purified through a protein A column, followed by peptide affinity purification.
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
582
Applicable Applications for anti-SHC2 antibody
Immunofluorescence (IF), Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA)
Application Notes
IF: 1:25
FC: 1:25
WB: 1:2000
FC: 1:25
WB: 1:2000
Antigen Type
Synthesized Peptide
Antigen Source
Human
Tissue Location
Expressed in brain. Expressed at high level in the hypothalamus and at low level in the caudate nucleus
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22349a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22349a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22349a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22349a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Western Blot (WB)
(All lanes : Anti-SHC2 Antibody (N-Term) at 1:2000 dilutionLane 1: Human cerebellum lysateLane 2: U-251 MG whole cell lysateLane 3: U-87 MG whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-SHC2 Antibody (N-Term) at 1:2000 dilutionLane 1: Human cerebellum lysateLane 2: U-251 MG whole cell lysateLane 3: U-87 MG whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-SHC2 antibody
Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons (By similarity).
Product Categories/Family for anti-SHC2 antibody
References
Grimwood J.,et al.Nature 428:529-535(2004). Nakamura T.,et al.J. Biol. Chem. 273:6960-6967(1998). Pelicci G.,et al.Oncogene 13:633-641(1996). Kavanaugh W.M.,et al.Science 266:1862-1865(1994). Liu H.Y.,et al.J. Biol. Chem. 277:26046-26056(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61916
NCBI Official Full Name
SHC-transforming protein 2
NCBI Official Synonym Full Names
SHC adaptor protein 2
NCBI Official Symbol
SHC2
NCBI Official Synonym Symbols
SCK; SLI; SHCB
NCBI Protein Information
SHC-transforming protein 2
UniProt Protein Name
SHC-transforming protein 2
Protein Family
UniProt Gene Name
SHC2
UniProt Synonym Gene Names
SCK; SHCB; SH2 domain protein C2
Uniprot Description
Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons ().
Research Articles on SHC2
Similar Products
Product Notes
The SHC2 shc2 (Catalog #AAA9231297) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHC2 Antibody (N-Term) reacts with Human Predicted: Human and may cross-react with other species as described in the data sheet. AAA Biotech's SHC2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA). IF: 1:25 FC: 1:25 WB: 1:2000. Researchers should empirically determine the suitability of the SHC2 shc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
