Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 1F2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
Mouse KDEL Monoclonal Antibody | anti-KDEL antibody
KDEL Antibody, Clone 1F2: PerCP
Reactivity
Human; Mouse; Rat
Applications
Western Blot
Purity
Protein G Purified
Synonyms
KDEL; Monoclonal Antibody; KDEL Antibody; Clone 1F2: PerCP; Mouse Anti-KDEL Monoclonal IgG1; KDEL Antibody: PerCP; Lys Asp Glu Leu Antibody; Lys-Asp-Glu-Leu (KDEL) Antibody; Lys-Asp-Glu-Leu Antibody; Lysine-aspartic acid-glutamate-leucine Antibody; anti-KDEL antibody
Host
Mouse
Reactivity
Human; Mouse; Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1F2
Specificity
Detects KDEL tagged proteins.
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-KDEL antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Target
KDEL
Immunogen
KDEL peptide (C-AVKDEL-COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
Conjugate
PerCP
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 1F2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
Western Blot (WB)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 1F2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
Related Product Information for anti-KDEL antibody
The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
Product Categories/Family for anti-KDEL antibody
References
1. Ozawa K., et al. (2008) Mol Pharmacol. 74:1610. 2. Austin R.C., et al. (2003) J Biol Chem. 278: 17438.
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Product Notes
The KDEL (Catalog #AAA809075) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KDEL Antibody, Clone 1F2: PerCP reacts with Human; Mouse; Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KDEL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the KDEL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDEL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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