Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22336a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22336a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Rabbit anti-Human GCA Polyclonal Antibody | anti-GCA antibody
GCA Antibody (N-Term)
FC: 1:25
WB: 1:2000
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22336a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22336a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22336a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22336a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Western Blot (WB)
(All lanes : Anti-GCA Antibody (N-Term) at 1:2000 dilutionLane 1: Human cerebellum lysateLane 2: Human spleen lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GCA Antibody (N-Term) at 1:2000 dilutionLane 1: Human cerebellum lysateLane 2: Human spleen lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a calcium-binding protein that is abundant in neutrophils and macrophages. In the absence of divalent cation, this protein localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions. Alternative splicing and use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Uniprot Description
Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.
Research Articles on GCA
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Product Notes
The GCA gca (Catalog #AAA9231283) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GCA Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GCA can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA). IF: 1:25 FC: 1:25 WB: 1:2000. Researchers should empirically determine the suitability of the GCA gca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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