Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
5611 results for "Protein A IgG Binding Buffer" - showing 2800-2850
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PDGFR antibody)
PDGFR, Polyclonal Antibody (Cat# AAA5303476)
Full Name
PDGFR antibody
Applications
IHC, IF, EIA
Purity
PDGFR antibody was purified by affinity chromatography
Pricing
$405/0.1 mg | $1,805/5x0.1 mg
Testing Data
(Detection of cleaved CYR61 in MDA-MB-231 cell lysate using MBS618208 Photo courtesy of Dr. Lupu's Lab, Northwestern University. Image by Dr. Ingrid Espinoza.)
Cysteine-rich, Angiogenic Inducer 61, Polyclonal Antibody (Cat# AAA618208)
Full Name
Cysteine-rich, Angiogenic Inducer 61 (CYR61)
Gene Names
CYR61; CCN1; GIG1; IGFBP10
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$660/0.1 mL | $2,965/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using P4HB antibody.)
P4HB, Polyclonal Antibody (Cat# AAA2523689)
Full Name
P4HB Antibody
Gene Names
P4HB; DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Antigen affinity purification
Pricing
$415/0.2 mL | $280/0.12 mL | $205/0.06 mL | $1,810/5x0.2 mL
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with CD49b antibody (FITC) and 0.125 ug of Armenian Hamster IgG Isotype Control (biotin) (left) or 0.125 ug of CD314 antibody (biotin) (right) followed by Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.)
NKG2D, Monoclonal Antibody (Cat# AAA834983)
Full Name
NKG2D antibody (biotin)
Applications
FC/FACS
Pricing
$595/0.1 mg | $2,665/5x0.1 mg
Testing Data
()
LYVE1, Polyclonal Antibody (Cat# AAA535139)
Full Name
LYVE1 antibody
Gene Names
LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1
Reactivity
Murine LYVE1
Applications
EIA, FC/FACS, WB
Purity
LYVE1 antibody was purified by Protein A affinity chromatography.
Pricing
$480/0.1 mg | $2,150/5x0.1 mg
Flow Cytometry (FC/FACS)
(Surface staining of MC/9 cell line with Fc epsilon Receptor I alpha antibody (FITC) (left) (PE) (right). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.)
FceRI alpha, Monoclonal Antibody (Cat# AAA833410)
Full Name
FceRI alpha antibody
Applications
FC/FACS
Purity
FceRI alpha antibody was purified by affinity chromatography.
Pricing
$595/0.1 mg | $2,680/5x0.1 mg
MOUSE IgG2a NEGATIVE CONTROL, Negative Control (Cat# AAA238094)
Full Name
MOUSE IgG2a NEGATIVE CONTROL:APC
Applications
FC/FACS
Pricing
$300/100 Tests | $1,195/5x100 Tests
muIgFc, Protein (Cat# AAA666811)
Full Name
muIgFc-Purified (with Antibiotics)
Reactivity
Human
Applications
FC/FACS, EIA
Purity
Purified (with Antibiotics)
Pricing
$505/0.025 mg | $2,235/5x0.025 mg
Western Blot (WB)
(Western blot All lanes: HCLS1-associated protein X-1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 31 kDa Observed band size: 40kDa Additional bands at: 90 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
HCLS1-associated protein X-1, Polyclonal Antibody (Cat# AAA715998)
Full Name
Rabbit anti-human HCLS1-associated protein X-1 polyclonal Antibody, FITC
Gene Names
HAX1; SCN3; HS1BP1; HCLSBP1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
LYVE1, Polyclonal Antibody (Cat# AAA534542)
Full Name
LYVE1 antibody
Gene Names
LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1
Reactivity
Murine LYVE1
Applications
EIA, FC/FACS, WB
Purity
LYVE1 antibody was purified by antigen affinity chromatography.
Pricing
$565/0.05 mg | $2,540/5x0.05 mg
Testing Data #1
(Western blot analysis of whole cell lysates from K562 (K) and A549 (A) cells probed with Rabbit anti BIM (MBS221821))
BIM, Polyclonal Antibody (Cat# AAA221821)
Full Name
RABBIT ANTI BIM
Gene Names
BCL2L11; BAM; BIM; BOD
Reactivity
Mouse (Expected from Sequence), Rat (Expected from Sequence)
Applications
WB
Pricing
$420/0.1 mg | $1,735/5x0.1 mg
Testing Data #1
(Western blot analysis of mouse skeletal muscle lysate probed with Rabbit anti Human SHP2 (MBS221486) at 0.5(A), 1(B) and 2(C) ug/ml)
SHP2, Polyclonal Antibody (Cat# AAA221486)
Full Name
RABBIT ANTI SHP2 (C-TERMINAL)
Gene Names
PTPN11; CFC; NS1; SHP2; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3
Reactivity
Mouse, Rat
Applications
WB
Pricing
$430/0.1 mg | $1,780/5x0.1 mg
Western Blot (WB)
(Western Blot analysis in human skeletal muscle lysate (20ug/lane) using MBS6010216 (1:2000). Secondary IgG (HRP) Goat Anti-Rabbit at 1:10000 dilution. Predicted band size: 19kD Blocking/Dilution buffer: 5% NFDM/TBST.)
DDIT3, Polyclonal Antibody (Cat# AAA6010216)
Full Name
DDIT3, CT (A135) (DDIT3, CHOP, CHOP10, GADD153, DNA damage-inducible transcript 3 protein, C/EBP-homologous protein, C/EBP-homologous protein 10, Growth arrest and DNA damage-inducible protein GADD153)
Gene Names
DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
Reactivity
Human, Mouse
Applications
EL/EIA, WB, FC/FACS
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$665/0.2 mL | $2,975/5x0.2 mL
Western Blot (WB)
(Western blot analysis of GRINA in human testis tissue lysate with GRINA antibody at (A) 1 and (B) 2 μg/mL.)
GRINA, Polyclonal Antibody (Cat# AAA153567)
Full Name
GRINA Antibody
Gene Names
GRINA; LFG1; HNRGW; TMBIM3; NMDARA1
Reactivity
Human
Applications
EIA, WB
Purity
GRINA Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Testing Data
CD36, Monoclonal Antibody (Cat# AAA666306)
Full Name
anti-human CD36-FITC
Gene Names
CD36; FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
Reactivity
Human
Applications
FC/FACS, IP
Pricing
$340/120 Tests | $1,480/5x120 Tests
Western Blot (WB)
(Western blot analysis of GAI15 expression in 3T3 cell lysate.)
GABP, Polyclonal Antibody (Cat# AAA835851)
Full Name
GABP antibody
Applications
WB
Purity
GABP antibody was purified by affinity chromatography
Pricing
$480/0.1 mg | $2,145/5x0.1 mg
Western Blot (WB)
(TP53 antibody (MBS830983) used at 1 ug/ml to detect target protein.)
TP53, Monoclonal Antibody (Cat# AAA830983)
Full Name
TP53 antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Applications
WB
Testing Data
(HUVEC cells were stained with MBS632005 (filled histogram) or APC-conjugated mouse isotype control (open histogram).)
LYVE1, Monoclonal Antibody (Cat# AAA632005)
Full Name
LYVE1 (Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1, LYVE-1, Cell Surface Retention Sequence-binding Protein 1, CRSBP-1, Extracellular Link Domain-containing Protein 1, Hyaluronic Acid Receptor, CRSBP1, HAR, XLKD1, UNQ230/PRO263) (APC)
Gene Names
LYVE1; XLKD1; LYVE-1; CRSBP-1
Reactivity
Human
Applications
FC/FACS
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$775/100 Tests | $3,485/5x100 Tests
p58, Recombinant Protein (Cat# AAA144863)
Full Name
Recombinant Borrelia Garinii p58
Gene Names
PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
Applications
WB
Purity
Greater than 80.0% as determined by SDS-PAGE.
Pricing
$250/0.02 mg | $3,725/1 mg | $180/0.005 mg | $16,535/5x1 mg
Western Blot (WB)
(Western blot analysis of Hsp47 expression in mouse small intestine tissue lysate.)
Hsp47, Polyclonal Antibody (Cat# AAA835082)
Full Name
Hsp47 antibody
Applications
IHC, IP, WB
Purity
Hsp47 antibody was purified by affinity chromatography
Pricing
$495/0.1 mg | $2,215/5x0.1 mg
MOUSE IgG2a NEGATIVE CONTROL, Negative Control (Cat# AAA238172)
Full Name
MOUSE IgG2a NEGATIVE CONTROL
Applications
FC/FACS
Pricing
$310/100 Tests | $1,235/5x100 Tests
Immunohistochemistry (IHC)
(H2034-51I(2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Heterochromatin Protein 1, beta, Polyclonal Antibody (Cat# AAA622871)
Full Name
Heterochromatin Protein 1, beta (HP1-beta, Chromobox Homolog 1, CBX1, Heterochromatin Protein p25 beta, HP1Hs-beta, M31, Modifier 1 Protein, MOD1, p25beta)
Gene Names
Su(var)205; CBX5; CG8409; dHP-1a; dHP1; DmelCG8409; DmHP-1; DmHP1; E(var)29; E(var)29A; FBgn0003607; H3; HP-1; hp1; Hp1; HP1; HP1-VS; hp1a; Hp1a; HP1a; HP1A; HP1alpha; Su(var); Su(var)2-05; Su(var)2-5; Su(Var)2-5; Su(var)2-501; su(var)205; SU(VAR)205; Su(var)29A; Su(
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Pricing
$620/0.1 mg | $2,775/5x0.1 mg
Testing Data
CD23, Monoclonal Antibody (Cat# AAA666117)
Full Name
anti-human CD23-R-PE
Gene Names
Fcer2; CD23; Fcer2a
Reactivity
Human
Applications
FC/FACS, WB
Pricing
$435/120 Tests | $1,920/5x120 Tests
Western Blot (WB)
(Western Blot of Jurkat lysate (35ug protein in RIPA buffer) using MBS621586 (0.3ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.)
TARDBP, Polyclonal Antibody (Cat# AAA621586)
Full Name
TARDBP (TAR DNA Binding Protein, ALS10, TDP-43, OTTHUMP00000002171, TAR DNA-binding protein-43)
Gene Names
TARDBP; ALS10; TDP-43; TARDBP
Reactivity
Human
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$620/0.1 mg | $2,775/5x0.1 mg
Testing Data #1
(Western blot analysis of whole cell lysate from Jurkat T-cell leukemia cells probed with Rabbit anti Human TIM-4 (MBS221476) at 1(A) and 2(B) ug/ml)
TIM-4, Polyclonal Antibody (Cat# AAA221476)
Full Name
RABBIT ANTI TIM-4 (C-TERMINAL)
Gene Names
TIMD4; TIM4; SMUCKLER
Reactivity
Mouse, Rat
Applications
WB
Pricing
$645/0.1 mg | $2,745/5x0.1 mg
Flow Cytometry (FC/FACS)
(Staining of unstimulated (orange histogram) or 3-day CD3 antibody and CD28 antibody stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of CD279 antibody (FITC). Total viable cells, as determined by Fixable Viability Dye (eFluor 450), were used for analysis.)
PD1, Monoclonal Antibody (Cat# AAA832834)
Full Name
PD1 antibody (FITC)
Gene Names
PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Applications
FC/FACS
Pricing
$365/0.05 mg | $1,630/5x0.05 mg
Western Blot (WB)
()
TR2, Polyclonal Antibody (Cat# AAA837656)
Full Name
TR2 antibody
Gene Names
Nr2c1; TR2; 80.3; Eenr; Tr2-11; 4831444H07Rik
Applications
WB
Purity
TR2 antibody was purified by Protein A affinity chromatography
Raf, Polyclonal Antibody (Cat# AAA835551)
Full Name
Phospho-Raf antibody
Applications
IHC, WB
Purity
Phospho-Raf antibody was purified by affinity chromatography
Pricing
$625/0.1 mg | $2,795/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using FOLR1 antibody. Secondary antibody: HRP Goat Anti-Rabbit IgG (H + L) at 1: 10000 dilution. Lysates / proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.)
FOLR1, Antibody (Cat# AAA8503555)
Full Name
FOLR1 Polyclonal Antibody
Gene Names
FOLR1; FBP; FOLR
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Affinity purification
Pricing
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot
Ras-related GTP-binding protein A, Polyclonal Antibody (Cat# AAA715788)
Full Name
Rabbit anti-human Ras-related GTP-binding protein A polyclonal Antibody, FITC
Gene Names
RRAGA; FIP1; RAGA; FIP-1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western Blot using MBS645437 (1:2000) Lane 1: K562 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 17kD Blocking/Dilution buffer: 5% NFDM/TBST.)
AIF1, Polyclonal Antibody (Cat# AAA645437)
Full Name
AIF1, NT (AIF1, G1, IBA1, Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1)
Gene Names
AIF1; IBA1; IRT1; AIF-1; IRT-1
Reactivity
Human, Mouse
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Testing Data
CD45, Monoclonal Antibody (Cat# AAA666407)
Full Name
anti-human CD45 RO-Purified (with Antibiotics)
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human, Primate, Monkey
Applications
FC/FACS
Purity
Purified (with Antibiotics)
Pricing
$300/0.1 mg | $1,295/5x0.1 mg
MIP-1 ALPHA, Polyclonal Antibody (Cat# AAA221275)
Full Name
RABBIT ANTI MOUSE MIP-1 ALPHA
Gene Names
Ccl3; Mip1a; Scya3; G0S19-1; AI323804; MIP1-(a); LD78alpha; MIP-1alpha; MIP1-alpha
Applications
EIA, WB
Pricing
$610/0.1 mg | $2,595/5x0.1 mg
Western Blot (WB)
(Western Blot analysis using MBS625991 Lane 1: A2058 cell line (35ug/lane) Lane 2: mouse brain tissue (35ug/lane). PKM2 (arrow) was detected using MBS625991.)
PKM2, Polyclonal Antibody (Cat# AAA625991)
Full Name
PKM2, CT (N491) (Pyruvate Kinase, Isozymes M1/M2, Pyruvate Kinase Muscle Isozyme, Cytosolic Thyroid Hormone-binding Protein, CTHBP, Opa-interacting Protein 3, OIP-3, Pyruvate Kinase 2/3, Thyroid Hormone-binding Protein 1, THBP1, Tumor M2-PK, p58, PKM, OIP
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1
Reactivity
Human, Mouse
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$665/0.2 mL | $2,975/5x0.2 mL
CD278, Protein (Cat# AAA666816)
Full Name
CD278(ICOS)-muIg-Purified Preservative Free
Reactivity
Human
Applications
FC/FACS, EIA
Purity
Purified Preservative Free
Pricing
$505/0.025 mg | $2,235/5x0.025 mg
Western Blot (WB)
(Western blot All lanes: Nuclear factor NF-kappa-B p105 subunit antibody at 2/mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size:50 kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Nuclear factor NF-kappa-B p105 subunit 1, Polyclonal Antibody (Cat# AAA719912)
Full Name
Rabbit anti-human Nuclear factor NF-kappa-B p105 subunit 1 polyclonal Antibody, FITC
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data
CD23, Monoclonal Antibody (Cat# AAA666068)
Full Name
anti-human CD23-Biotin
Gene Names
Fcer2; CD23; Fcer2a
Reactivity
Human
Applications
FC/FACS, WB
Pricing
$340/0.1 mg | $1,480/5x0.1 mg
Testing Data
CD94, Monoclonal Antibody (Cat# AAA666268)
Full Name
anti-human CD94-FITC
Gene Names
KLRD1; CD94
Reactivity
Human
Applications
FC/FACS
Pricing
$475/120 Tests | $2,085/5x120 Tests
Western Blot (WB)
(Western Blot analysis of Rat brain membrane lysate showing detection of SHANK protein using Mouse Anti-SHANK Monoclonal Antibody, Clone S23b-49. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Shank, Monoclonal Antibody (Cat# AAA800310)
Full Name
SHANK (pan) Antibody: Alkaline Phosphatase
Gene Names
Shank2; CortBP1; ProSAP1; Spank-3
Reactivity
Mouse, Human, Rat
Applications
WB, IHC, IP
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
RAF1, Polyclonal Antibody (Cat# AAA1489353)
Full Name
Rabbit Anti Human RAF1 PolyClonal Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$325/0.1 mL | $1,465/5x0.1 mL
Western Blot (WB)
(Western blot analysis of TRAF3 in 3T3 cell lysate with TRAF3 antibody at (A) 1, and (B) 2 μg/mL.)
TRAF3, Polyclonal Antibody (Cat# AAA151227)
Full Name
TRAF3 Antibody
Gene Names
TRAF3; CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp
Reactivity
Human, Mouse
Applications
EIA, WB, IF
Purity
TRAF3 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
MAPKAPK 2, Polyclonal Antibody (Cat# AAA837314)
Full Name
MAPKAPK 2 antibody
Gene Names
Mapkapk2; MK2; MK-2; Rps6kc1; AA960234; MAPKAP-K2
Applications
IP, WB
Purity
MAPKAPK 2 antibody was purified by affinity purification.
Western Blot (WB)
(Western blot All lanes: Nuclear factor NF-kappa-B p105 subunit antibody at 2/mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size:50 kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Nuclear factor NF-kappa-B p105 subunit 1, Polyclonal Antibody (Cat# AAA719966)
Full Name
Rabbit anti-human Nuclear factor NF-kappa-B p105 subunit 1 polyclonal Antibody, Biotin conjugated
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Flow Cytometry (FC/FACS)
(Staining of the MC/9 cell line with 0.06 ug of Armenian Hamster IgG Isotype Control (FITC) (open histogram) or 0.06 ug of Fc epsilon Receptor I alpha antibody (FITC) (filled histogram). Total viable cells were used for analysis.)
FceRI alpha, Monoclonal Antibody (Cat# AAA834069)
Full Name
FceRI alpha antibody (FITC)
Applications
FC/FACS
Pricing
$315/0.05 mg | $1,415/5x0.05 mg
Serine/threonine-protein kinase TBK1, Polyclonal Antibody (Cat# AAA1489364)
Full Name
Rabbit anti-human Serine/threonine-protein kinase TBK1 polyclonal Antibody; Biotin conjugated
Gene Names
TBK1; NAK; T2K
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
CYBB, Polyclonal Antibody (Cat# AAA1491109)
Full Name
Rabbit Anti Human CYBB PolyClonal Antibody
Gene Names
CYBB; CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$320/0.1 mL | $1,440/5x0.1 mL
SDS-Page
(Peptide Competition Extracts prepared from 3T3L1 cells left unstimulated (1) or stimulated with LIF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4ºC, then were incubated with MBS628693 for two hours at RT in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the phosphopeptide immunogen (2), a generic phosphotyrosine-containing peptide (3), or, the non-phosphopeptide corresponding to the phosphopeptide immunogen (4).After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRPconjugate and bands were detected using the Pierce SuperSignal method. The data show that only the phosphopeptide corresponding to STAT3 [pY705] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the induction of phosphorylation by the addition of LIF.)
STAT3, Polyclonal Antibody (Cat# AAA628693)
Full Name
STAT3, phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF)
Gene Names
STAT3; APRF; HIES
Reactivity
Human, Mouse, Rat
Applications
EL/EIA, WB, FC/FACS
Purity
Affinity Purified
Purified by immunoaffinity chromatography
Purified by immunoaffinity chromatography
Pricing
$695/10 Blots | $3,110/5x10 Blots
Chemiluminescence
(MBS621895 (0.01ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
HMG4, Polyclonal Antibody (Cat# AAA621895)
Full Name
HMG4 (High Mobility Group 4 (HMG) Transcription Factor, High Mobility Group Protein 2a, HMG2a, High Mobility Group Box 3, HMGB3, MGC90319, Nonhistone Chromosomal Protein HMG4)
Gene Names
HMGB3; HMG4; HMG-4; HMG2A; HMG-2a
Reactivity
Human
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by antigen immunoaffinity chromatography.
Purified by antigen immunoaffinity chromatography.
Pricing
$620/0.1 mg | $2,775/5x0.1 mg
SLP-76, Monoclonal Antibody (Cat# AAA212748)
Full Name
MOUSE ANTI SLP-76
Gene Names
LCP2; SLP76; SLP-76
Reactivity
Mouse, Pig
Applications
IP, WB
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Ubiquitin thioesterase OTUB1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 20 kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Ubiquitin thioesterase OTUB1, Polyclonal Antibody (Cat# AAA719635)
Full Name
Rabbit anti-human Ubiquitin thioesterase OTUB1 polyclonal Antibody, HRP conjugated
Gene Names
OTUB1; OTB1; OTU1; HSPC263
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
