Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with CD49b antibody (FITC) and 0.125 ug of Armenian Hamster IgG Isotype Control (biotin) (left) or 0.125 ug of CD314 antibody (biotin) (right) followed by Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.)
Hamster NKG2D Monoclonal Antibody | anti-NKG2D antibody
NKG2D antibody (biotin)
Applications
Flow Cytometry, Functional Assay
Synonyms
NKG2D; Monoclonal Antibody; NKG2D antibody (biotin); Monoclonal NKG2D; Anti-NKG2D; NKGD-2; NKGD 2; KLRK1; anti-NKG2D antibody
Host
Hamster
Clonality
Monoclonal
Isotype
IgG
Clone Number
A10
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-NKG2D antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.125 ug/test
This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
The A10 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1 alpha, beta, gamma, delta, epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1 thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells. A10 and C7 antibodies detect NK cells from all inbred strains of mice tested so far. In redirected lysis assays, A10 is reported to stimulate NK cells to kill Daudi targets. In addition, immobilized A10 is reported to induce production of GM-CSF by NK clones and LAK cells through a CD16 concomitant engagement. A10 and another hamster anti-mouse NKG2D compete with each other for binding to transfected cells by flow cytometric analysis, suggesting that they may bind to similar epitopes or block each other by steric hindrance. C7 and A10 also exhibit different functional properties. Expression of the NKG2D antigen on mouse peripheral NK and NKT cells can be detected by flow cytometric analysis using mAb CX5 with much brighter intensity.
Tag/Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with CD49b antibody (FITC) and 0.125 ug of Armenian Hamster IgG Isotype Control (biotin) (left) or 0.125 ug of CD314 antibody (biotin) (right) followed by Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with CD49b antibody (FITC) and 0.125 ug of Armenian Hamster IgG Isotype Control (biotin) (left) or 0.125 ug of CD314 antibody (biotin) (right) followed by Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.)
Related Product Information for anti-NKG2D antibody
Armenian Hamster monoclonal NKG2D antibody (biotin)
Product Categories/Family for anti-NKG2D antibody
Similar Products
Product Notes
The NKG2D (Catalog #AAA834983) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NKG2D can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.125 ug/test This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the NKG2D for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NKG2D, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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