Mouse anti-Human LYVE1 Monoclonal Antibody | anti-LYVE1 antibody

LYVE1 (Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1, LYVE-1, Cell Surface Retention Sequence-binding Protein 1, CRSBP-1, Extracellular Link Domain-containing Protein 1, Hyaluronic Acid Receptor, CRSBP1, HAR, XLKD1, UNQ230/PRO263) (APC)

Cat. Num. AAA632005

• Gene Names: LYVE1; XLKD1; LYVE-1; CRSBP-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: LYVE1; Monoclonal Antibody; LYVE1 (Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1; LYVE-1; Cell Surface Retention Sequence-binding Protein 1; CRSBP-1; Extracellular Link Domain-containing Protein 1; Hyaluronic Acid Receptor; CRSBP1; HAR; XLKD1; UNQ230/PRO263) (APC); Anti -LYVE1 (Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1; anti-LYVE1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10K86
• Specificity: Recognizes human LYVE-1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in 1ml saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-LYVE1 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: 10ul labels up to 1x10e6 cells. Validated for use on HUVEC cells.; Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.; 2. After blocking, add 10ul of L9375-01B to up to 1x10e6 cells and incubate for 30 minutes at RT.; 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.; 4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG1 antibody.
• Immunogen: Recombinant protein corresponding to aa20-238 from human LYVE1 expressed in E. coli (Q9Y5Y7).
• Preparation and Storage: May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Stable for at 12 months at 4 degree C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyanin conjugates are sensitive to light.

Testing Data

(HUVEC cells were stained with MBS632005 (filled histogram) or APC-conjugated mouse isotype control (open histogram).)

Related Product Information for anti-LYVE1 antibody

LYVE1, also named extracellular link domain containing 1, is a 60-70kD type I transmembrane protein that functions as a receptor for both soluble and immobilized hyaluronan (HA). It shares 41% aa sequence homology with CD44, another known receptor for HA. LYVE1 expression is mostly restricted to lymphatic vessels. LYVE1 is a useful molecular marker for lymphatic endothelial cells.

Product Categories/Family for anti-LYVE1 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 147904924
• NCBI GeneID: 404179
• NCBI Accession #: NP_991384.2
• NCBI GenBank Nucleotide #: NM_205815.2
• UniProt Accession #: Q6UC88
• Molecular Weight: 35,561 Da
• NCBI Official Full Name: lymphatic vessel endothelial hyaluronic acid receptor 1
• NCBI Official Synonym Full Names: lymphatic vessel endothelial hyaluronan receptor 1
• NCBI Official Symbol: LYVE1
• NCBI Official Synonym Symbols: XLKD1; LYVE-1; CRSBP-1
• NCBI Protein Information: lymphatic vessel endothelial hyaluronic acid receptor 1; extracellular link domain containing 1; lymphatic vessel endothelial HA recptor-1; extracellular link domain-containing protein 1; cell surface retention sequence binding protein-1; cell surface retention sequence-binding protein 1
• UniProt Protein Name: Lymphatic vessel endothelial hyaluronic acid receptor 1
• Protein Family: Lymphatic vessel endothelial hyaluronic acid receptor
• UniProt Gene Name: LYVE1
• UniProt Synonym Gene Names: CRSBP1; XLKD1; LYVE-1; CRSBP-1
• UniProt Entry Name: LYVE1_BOVIN

Uniprot Description

Function: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. Ref.1

Subunit structure: Homodimer; disulfide-linked. Interacts with PDGFB and IGFBP3. Forms a transient ternary complex with PDGFB and PDGFRB in TGN

By similarity. Ref.1

Subcellular location: Membrane; Single-pass membrane protein

Potential. Note: Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface

By similarity.

Post-translational modification: N-glycosylated. Ref.1O-glycosylated. Ref.1

Sequence similarities: Contains 1 Link domain.