Western Blot (WB)
(Western Blot of Jurkat lysate (35ug protein in RIPA buffer) using MBS621586 (0.3ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat anti-Human TARDBP Polyclonal Antibody | anti-TARDBP antibody
TARDBP (TAR DNA Binding Protein, ALS10, TDP-43, OTTHUMP00000002171, TAR DNA-binding protein-43)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Peptide ELISA: 1:16,000
Western Blot: 0.3-1ug/ml Detects ~45kD band in human Jurkat cell lysates.
Western Blot (WB)
(Western Blot of Jurkat lysate (35ug protein in RIPA buffer) using MBS621586 (0.3ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(Western Blot of Jurkat lysate (35ug protein in RIPA buffer) using MBS621586 (0.3ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]
Uniprot Description
TARDBP: DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR. Homodimer. Binds specifically to pyrimidine-rich motifs of TAR DNA and to single stranded TG repeated sequences. Binds to RNA, specifically to UG repeated sequences with a minimun of six contiguous repeats. Interacts with ATNX2; the interaction is RNA-dependent. Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding
Chromosomal Location of Human Ortholog: 1p36.22
Cellular Component: nucleoplasm; perichromatin fibrils; cytoplasm; nuclear speck; nucleus
Molecular Function: identical protein binding; protein binding; mRNA 3'-UTR binding; RNA binding; double-stranded DNA binding; nucleotide binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; RNA splicing; positive regulation of insulin secretion; positive regulation of transcription from RNA polymerase II promoter; mRNA processing
Disease: Amyotrophic Lateral Sclerosis 10, With Or Without Frontotemporal Dementia
Research Articles on TARDBP
2. characteristic clinical phenotype of familial amyotrophic lateral sclerosis with initial bulbar symptoms occurred in a family with a TDP-43 M337V substitution
3. TDP-43 plays a role in miRNA expression pathways. Let-7b and miR-663 expression levels are down- and upregulated, respectively. Both miRNAs are capable of binding directly to TDP-43 in different positions.
4. TDP-43 exerts cell-autonomous neurotoxicity in Drosophila; dose-dependent alterations of human TDP-43 nuclear function may underlie motor neuron death in ALS
5. To clarify the conformational and physical properties of the TDP-43 disordered C-terminal domain, several peptide fragments were synthesized and shown that only D1 within D1-4 can form twisted fibrils
6. Both a gain and loss of function of mutant TDP-43 are involved in contributes to Amyotrophic Lateral Sclerosis pathogenesis.
7. These findings suggest that approximately 25-kDa TDP-43 C-terminal fragments are noxious to neurons by a gain of aberrant nuclear function.
8. TDP-43 and TDP-25 are degraded by both proteasome and autophagy with TDP-25 being more regulated.
9. The spectrum of TARDBP mutations and polymorphisms supported the pathological role of TDP-43 in Chinese amyotrophic lateral sclerosis patients.
10. TARDBP variants may result in clinically and neuropathologically heterogeneous phenotypes linked by a common molecular pathology in frontotemporal lobar degeneration.
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Product Notes
The TARDBP tardbp (Catalog #AAA621586) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The TARDBP (TAR DNA Binding Protein, ALS10, TDP-43, OTTHUMP00000002171, TAR DNA-binding protein-43) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TARDBP can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Peptide ELISA: 1:16,000 Western Blot: 0.3-1ug/ml Detects ~45kD band in human Jurkat cell lysates. Researchers should empirically determine the suitability of the TARDBP tardbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TARDBP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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