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1532 results for "Rabbit IgG Isotype Control" - showing 200-250


Flow Cytometry (FC/FACS) (Flow cytometric analysis of Hela cells using RPS6 Antibody (N-term)(green) compared to an isotype control of mouse IgG1(blue). MBS9200615 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)

RPS6, Monoclonal Antibody (Cat# AAA9200615)

Full Name
RPS6 Antibody (N-term)
Gene Names
RPS6; S6
Reactivity
Human, rat (Predicted Reactivity: Monkey, Mouse)
Applications
EIA, IHC, WB, FC/FACS, IF
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (All lanes : Anti-MICA Antibody (Center) at 1:2000 dilutionLane 1: A431 whole cell lysatesLane 2: U-87 MG whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

MICA, Polyclonal Antibody (Cat# AAA9206685)

Full Name
MICA Antibody (Center)
Gene Names
MICA; MIC-A; PERB11.1
Reactivity
Human
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Flow Cytometry (FC/FACS) (Flow cytometric analysis of SH-SY5Y cells using STRADA Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9203971 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

STRADA, Polyclonal Antibody (Cat# AAA9203971)

Full Name
STRADA Antibody (C-term)
Gene Names
STRADA; LYK5; PMSE; Stlk; STRAD; NY-BR-96
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Applications
WB, EIA, FC/FACS, IF, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Flow Cytometry (FC/FACS) (Flow-cytometry using anti-CD44 antibody Hermes-3 Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of Hermes-3 (MBS488447, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)

CD44, Monoclonal Recombinant Antibody (Cat# AAA488447)

Full Name
Anti-CD44 [Hermes -3]
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human
Applications
BL, WB, IHC, IF, FC/FACS, ICC, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS) (Flow-cytometry using the anti-desmosome/hemidesmosome antibody F12 A431 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of F12 (MBS488463, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

Desmosome/Hemidesmosome, Monoclonal Recombinant Antibody (Cat# AAA488463)

Full Name
Anti-Desmosome/Hemidesmosome [F12]
Reactivity
Human
Applications
IF, IEM
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB) (Figure 1. Western blot analysis of Bcl-X using anti- Bcl-X antibody (MBS178417). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Bcl-X antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Bcl-X at approximately 29 KD, 60KD. The expected band size for Bcl-X is at 26KD. )

Bcl-X, Polyclonal Antibody (Cat# AAA178417)

Full Name
Anti-Bcl-X Antibody
Gene Names
BCL2L1; BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
Reactivity
Human
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of Bik using anti-Bik antibody (MBS178226). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Spleen Tissue Lysate, Lane 2: Rat Gaster Tissue Lysate, Lane 3: Rat Intestine Tissue Lysate, Lane 4: MCF-7 Whole Cell Lysate, Lane 5: A549 Whole Cell Lysate, Lane 6: SKOV Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bik antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Bik at approximately 22KD. The expected band size for Bik is at 22KD. )

Bik, Polyclonal Antibody (Cat# AAA178226)

Full Name
Anti-Bik Antibody
Gene Names
BIK; BP4; NBK; BIP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of Dynamin 1 using anti-Dynamin 1 antibody (MBS1750776). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates,Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dynamin 1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Dynamin 1 at approximately 97KD. The expected band size for Dynamin 1 is at 97KD. )

Dynamin 1, Polyclonal Antibody (Cat# AAA1750776)

Full Name
Anti-Dynamin 1 Picoband antibody
Gene Names
DNM1; DNM; EIEE31
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western blot analysis of CD33 expression in SKOV3 whole cell lysates (lane 1). CD33 at 45KD; 67KD was detected using rabbit anti-CD33 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )

CD33, Polyclonal Antibody (Cat# AAA178432)

Full Name
Anti-CD33 Antibody
Gene Names
CD33; p67; SIGLEC3; SIGLEC-3
Reactivity
Human
Applications
WB, IHC, ICC, FC/FACS
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry (FC/FACS) (Figure-1: Cell surface flow analysis of PE conjugated Rabbit IgG Isotype control in Jurkat Cell line using 0.5/10^6 cells. Black represent cell alone, red represent Rabbit IgG Isotype control conjugated with PE (Cat No.-11-201-PE).)

IgG, Polyclonal Isotype Control (Cat# AAA669060)

Full Name
Rabbit IgG Isotype control- PE Conjugated
Applications
EIA, FC/FACS, IF
Purity
Purified
Affinity Chromatography
Pricing
$210/100 Tests | $895/5x100 Tests
Flow Cytometry (FC/FACS) ( Flow-cytometry using anti-CD33 antibody hP67.6 (Gemtuzumab) Human leukocytes were stained with an isotype control or the rabbit-chimeric version of hP67.6 (Gemtuzumab) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)

CD33, Monoclonal Recombinant Antibody (Cat# AAA488242)

Full Name
Anti-CD33 [hP67.6 (Gemtuzumab)]
Reactivity
Human
Applications
FC/FACS, IF
Purity
Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC) (Anti-TLR7 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

TLR7, Polyclonal Antibody (Cat# AAA247379)

Full Name
Rabbit Polyclonal (IgG) to Human TLR7
Gene Names
TLR7; TLR7-like
Reactivity
Chimpanzee, Human, Monkey
Applications
IHC - Paraffin, WB, FC/FACS
Purity
Protein G Purified
Pricing
$585/0.05 mg | $2,585/5x0.05 mg
Flow Cytometry (FC/FACS) (Overlay histogram showing Jurkat cells stained at 37 degree C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

AVPR2, Polyclonal Antibody (Cat# AAA9232002)

Full Name
AVPR2 Antibody (C-term)
Gene Names
AVPR2; DI1; DIR; NDI; V2R; ADHR; DIR3
Reactivity
Human
Applications
WB, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB) (Western blot analysis of lysates from Hela, NCI-H1299 cell line (from left to right),using RPL6 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

RPL6, Polyclonal Antibody (Cat# AAA9231604)

Full Name
RPL6 Antibody (N-term)
Gene Names
RPL6; L6; TXREB1; SHUJUN-2; TAXREB107
Reactivity
Human
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunohistochemistry (IHC) (Figure 1. IHC analysis of TAPA1 using anti-TAPA1 antibody (MBS1750607).TAPA1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TAPA1 Antibody (MBS1750607) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

TAPA1, Polyclonal Antibody (Cat# AAA1750607)

Full Name
Anti-TAPA1 Picoband antibody
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of NFIA using anti- NFIA antibody (MBS178836).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: JURKAT whole cell lysates,Lane 2: COLO320 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- NFIA antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFIA at approximately 69KD. The expected band size for NFIA is at 56KD. )

NFIA, Polyclonal Antibody (Cat# AAA178836)

Full Name
Anti-NFIA Antibody
Gene Names
NFIA; CTF; NF1-A; NFI-A; NFI-L; NF-I/A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC) (Anti-TLR7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

TLR7, Polyclonal Antibody (Cat# AAA241936)

Full Name
Rabbit Polyclonal (IgG) to Human TLR7
Gene Names
TLR7; TLR7-like
Reactivity
Human, Mouse
Applications
IHC - Paraffin; IHC - Frozen, WB, FC/FACS
Purity
Protein G Purified
Pricing
$610/0.05 mg | $2,685/5x0.05 mg
Western Blot (WB) (Western blot analysis of lysates from A431,KG-1 cell line,mouse kidney,mouse liver tissue lysate,SH-SY5Y cell line (from left to right), using CLN3 Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)

CLN3, Polyclonal Antibody (Cat# AAA9231898)

Full Name
CLN3 Antibody (Center)
Gene Names
CLN3; BTS; BTN1; JNCL
Reactivity
Human, Mouse
Predicted: Monkey
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Flow Cytometry (FC/FACS) (Overlay histogram showing Hela cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

RPS7, Polyclonal Antibody (Cat# AAA9232223)

Full Name
RPS7 Antibody (N-Term)
Gene Names
RPS7; S7; eS7; DBA8
Reactivity
Human, Mouse
Predicted: Bovine, Zebrafish, Rat, Xenopus
Applications
WB, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB) (Western blot analysis of lysates from A431,Hela cell line (from left to right), using DFFB Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

DFFB, Polyclonal Antibody (Cat# AAA9231709)

Full Name
DFFB Antibody (Center)
Gene Names
DFFB; CAD; CPAN; DFF2; DFF40; DFF-40
Reactivity
Human
Predicted: Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Flow Cytometry (FC/FACS) (Flow-cytometry using the anti-IL-18 antibody SAIC-24B-4 Human peripheral blood monocytes (PBMC’s) were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of SAIC-24B-4 (MBS488409, blue line) at a dilution of 1:100 for 1h at RT. An additional population of PBMC’s stimulated with PMA and Ionomycin in the presence of Monensin for 4 hours were stained in a similar manner (purple line). After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

IL-18, Monoclonal Recombinant Antibody (Cat# AAA488409)

Full Name
Anti-IL-18 [SAIC-24B-4]
Gene Names
IL18; IGIF; IL-18; IL-1g; IL1F4
Reactivity
Human
Applications
Immuno-MRM
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB) (Anti- BMP-5 Picoband antibody, MBS178341, Western blottingAll lanes: Anti BMP-5 (MBS178341) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Mouse Liver Tissue Lysate at 50ugLane 3: A549 Whole Cell Lysate at 40ugPredicted bind size: 51KDObserved bind size: 51KD)

BMP5, Polyclonal Antibody (Cat# AAA178341)

Full Name
Anti-BMP5 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF) (Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10 Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of LG.3A10 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)

CD27, Monoclonal Recombinant Antibody (Cat# AAA488454)

Full Name
Anti-CD27 [LG.3A10]
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Applications
IP, IF, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS) (Overlay histogram showing U-2OS cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

CF150, Polyclonal Antibody (Cat# AAA9231966)

Full Name
CF150 Antibody (Center)
Gene Names
CGAS; MB21D1; h-cGAS; C6orf150
Reactivity
Human
Applications
WB, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
ELISA (EIA) (ELISA of anti-TIGIT antibody on TIGIT-Fc fusion protein. Binding curves of the rabbit chimeric IgG version of the anti-TIGIT antibody 4D4 (MBS488471; red line) and isotype control to an ELISA plate coated with mouse TIGIT-Fc fusion protein (Pr00163-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using MBS488471. For signal detection, a 1:4000 dilution of HRP-labelled anti-rabbit IgG1 (BioRad 5196-2504) antibody was used. The second increase in absorbance at 1000ng/ml is suggestive of a possible secondary binding epitope for MBS488471 on Pr00163-1.9.)

TIGIT, Monoclonal Recombinant Antibody (Cat# AAA488471)

Full Name
Anti-TIGIT [4D4]
Gene Names
Tigit; Vstm3
Reactivity
Mouse
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg

hapten 4-hydroxy-3-nitrophenyl acetyl (NP), Recombinant Antibody (Cat# AAA488060)

Full Name
Anti-hapten 4-hydroxy-3-nitrophenyl acetyl (NP) [B1-8]
Applications
WB, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB) (Western blot analysis of lysates from mouse kidney and liver tissue lysate (from left to right), using Mouse Csnk1g3 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.)

Csnk1g3, Polyclonal Antibody (Cat# AAA9231587)

Full Name
Mouse Csnk1g3 Antibody (C-term)
Gene Names
Csnk1g3; 3300002K07Rik; C330049O21Rik
Reactivity
Mouse
Predicted: Rat
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of C-Kit using anti-C-Kit antibody (MBS177665).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: Recombinan Human C-Kit Protein 0.5ngAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C-Kit antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for C-Kit at approximately49KD. The expected band size for C-Kit is at 49KD. )

c-Kit, Polyclonal Antibody (Cat# AAA177665)

Full Name
Anti-c-Kit Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Reactivity
Human
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of NFATC4 using anti-NFATC4 antibody (MBS1750778).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat testis tissue lysates,Lane 2: mouse testis tissue lysates,Lane 3: human Hela whole cell lysates,Lane 4: human placenta tissue lysates,Lane 5: human A549 whole cell lysates,Lane 6: human SK-OV-3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFATC4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFATC4 at approximately 95120KD. The expected band size for NFATC4 is at 95KD. )

NFATC4, Polyclonal Antibody (Cat# AAA1750778)

Full Name
Anti-NFATC4 Picoband Antibody
Gene Names
NFATC4; NFAT3; NF-AT3; NF-ATC4
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of ATF2 using anti-ATF2 antibody (MBS177474).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: Recombinant Human ATF2 Protein 0.5ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATF2 at approximately 49KD. The expected band size for ATF2 is at 49KD. )

Cyclic AMP-dependent transcription factor ATF-2, Polyclonal Antibody (Cat# AAA177474)

Full Name
Anti-ATF2 Antibody
Gene Names
ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
WB, IHC-P, IHC-F
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western blot analysis of lysates from A431,mouse NIH/3T3,L929,rat C6,Hela cell line (from left to right), using HIST1H4A Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

HIST1H4A, Polyclonal Antibody (Cat# AAA9231704)

Full Name
HIST1H4A Antibody (C-term)
Reactivity
Human, Mouse, Rat
Predicted: Bovine, C.Elegans, Chicken, D, Monkey, Pig, Xenopus, Yeast
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Flow Cytometry (FC/FACS) (Overlay histogram showing A431 cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

SLC22A2, Polyclonal Antibody (Cat# AAA9232198)

Full Name
SLC22A2 Antibody (N-term)
Gene Names
SLC22A2; OCT2
Reactivity
Human
Applications
WB, IHC, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB) (Western blot analysis of APOBEC3G expression in A431 whole cell lysates (lane 1), and JURKAT whole cell lysates (lane 2). APOBEC3G at 46KD was detected using rabbit anti- APOBEC3G Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )

APOBEC3G, Polyclonal Antibody (Cat# AAA178485)

Full Name
Anti-APOBEC3G Antibody
Gene Names
APOBEC3G; A3G; ARCD; ARP9; ARP-9; CEM15; CEM-15; MDS019; bK150C2.7; dJ494G10.1
Reactivity
Human
No cross reactivity with other proteins.
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of Stefin B using anti-Stefin B antibody (M02794). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Stefin B antigen affinity purified monoclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.)

Stefin B, Monoclonal Antibody (Cat# AAA1752235)

Full Name
Anti-Stefin B Antibody (monoclonal, 2B6)
Gene Names
CSTB; PME; ULD; CST6; EPM1; STFB; CPI-B; EPM1A
Reactivity
Human
Applications
WB, IHC, ICC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (All lanes : Anti-MICA Antibody (Center) at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: Daudi whole cell lysatesLane 3: Jurkat whole cell lysatesLane 4: MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

MICA, Polyclonal Antibody (Cat# AAA9232043)

Full Name
MICA Antibody (Center)
Gene Names
MICA; MIC-A; PERB11.1
Reactivity
Human
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of CD147 using anti-CD147 antibody (MBS177907).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: Recombinant Human CD147 Protein 0.5ngAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD147 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD147 at approximately39KD. The expected band size for CD147 is at 39KD. )

CD147, Polyclonal Antibody (Cat# AAA177907)

Full Name
Anti-CD147 Antibody
Gene Names
BSG; OK; 5F7; TCSF; CD147; EMMPRIN
Reactivity
Human
Applications
WB
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry (FC/FACS) (Flow-cytometry using the anti-OX40L antibody R4930 (Oxelumab) (Ab00536) Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of R4930 (Ab00536-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

OX40L, Recombinant Antibody (Cat# AAA488213)

Full Name
Anti-OX40L [R4930 (Oxelumab)]
Gene Names
TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
Reactivity
Human
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF) ( Immunofluorescence staining of fixed MCF7 cells with anti-Breast epithelial mucin antibody Mc5 (Ab00345) Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Mc5 (Ab00345-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00345-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (Ab178-23.0) followed by Alexa Fluor® 488 secondary antibody.)

Breast Epithelial Mucin, Monoclonal Recombinant Antibody (Cat# AAA488302)

Full Name
Anti-Breast Epithelial Mucin [Mc5]
Reactivity
Human
Applications
EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS) (Overlay histogram showing U-87 MG cells stained at 37 degree C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

PLOD1, Polyclonal Antibody (Cat# AAA9231969)

Full Name
PLOD1 Antibody (N-term)
Gene Names
PLOD1; LH; LH1; LLH; EDS6; PLOD; EDSKCL1
Reactivity
Human
Predicted: Mouse, Rat
Applications
WB, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Flow Cytometry (FC/FACS) (Flow cytometric analysis of SH-SY5Y cells using Mouse Mapk11 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9202755 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

Mapk11, Polyclonal Antibody (Cat# AAA9202755)

Full Name
Mouse Mapk11 Antibody (N-term)
Gene Names
Mapk11; P38b; Sapk2; p38-2; Prkm11; Sapk2b; p38beta; p38beta2
Reactivity
Mouse (Predicted Reactivity: Human)
Applications
FC/FACS, EIA, IHC, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Flow Cytometry (FC/FACS) (Flow cytometric analysis of U-87 MG cells using HBG2 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9205274 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

HBG2, Polyclonal Antibody (Cat# AAA9205274)

Full Name
HBG2 Antibody (C-term)
Gene Names
HBG2; TNCY; HBG-T1
Reactivity
Human
Applications
WB, EIA, FC/FACS, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data (Anti-CD169 staining of murine macrophages Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD169 antibody (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a CD169-positive population is apparent.)

CD169, Monoclonal Recombinant Antibody (Cat# AAA488383)

Full Name
Anti-CD169 [3D6.112 (Recombinant Version)]
Gene Names
Siglec1; Sn; Cd169; Siglec-1
Reactivity
Mouse
Applications
WB, IHC
Purity
Protein A Affinity Purified
Pricing
$635/0.1 mg | $2,660/5x0.1 mg
Testing Data #1 (Human peripheral blood lymphocytes stained with Mouse anti Human siglec-10 (MBS224495) followed by Rabbit F(ab')2 anti Mouse IgG:FITC (MBS235160))

SIGLEC-10, Monoclonal Antibody (Cat# AAA224376)

Full Name
MOUSE ANTI HUMAN SIGLEC-10:FITC
Gene Names
SIGLEC10; SLG2; PRO940; SIGLEC-10
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of PPT1 using anti- PPT1 antibody (MBS178109).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates,Lane 3: rat liver tissue lysates,Lane 4: mouse liver tissue lysates,Lane 5: HEPG2 whole Cell lysates,Lane 6: 293T whole cell lysates,Lane 7: MCF-7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PPT1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PPT1. )

PPT1, Polyclonal Antibody (Cat# AAA178109)

Full Name
Anti-PPT1 Antibody
Gene Names
PPT1; PPT; CLN1; INCL
Reactivity
Human, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of DC-SIGN using anti-DC-SIGN antibody (MBS1750548).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: rat spleen tissue lysates,Lane 6: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DC-SIGN antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DC-SIGN at approximately 46KD. The expected band size for DC-SIGN is at 46KD. )

DC-SIGN, Polyclonal Antibody (Cat# AAA1750548)

Full Name
Anti-DC-SIGN Picoband antibody
Gene Names
CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg

Unknown Specificity (Isotype control), Recombinant Antibody (Cat# AAA488149)

Full Name
Anti-Unknown Specificity (Isotype control) [MOPC-21]
Applications
WB, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB) (Western blot analysis of lysates from HepG2,Jurkat,K562 cell line (from left to right), using Phospho-HUMAN-SHB(Y268).ctrl. It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)

SHB, Polyclonal Antibody (Cat# AAA9231710)

Full Name
HUMAN-SHB (Y268) Antibody
Gene Names
SHB; bA3J10.2
Reactivity
Human
Predicted: Mouse
Applications
WB, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB) (Western blot analysis of lysates from Hela, K562, MCF-7 cell line (from left to right), using EEF1B2 Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

EEF1B2, Polyclonal Antibody (Cat# AAA9231626)

Full Name
EEF1B2 Antibody (Center)
Gene Names
EEF1B2; EF1B; EEF1B; EEF1B1
Reactivity
Human
Predicted: Chicken
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Flow Cytometry (FC/FACS) (Flow cytometric analysis of K562 cells using CDK5 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9208751 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

CDK5, Polyclonal Antibody (Cat# AAA9208751)

Full Name
CDK5 Antibody (C-term)
Gene Names
CDK5; LIS7; PSSALRE
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat, Xenopus)
Applications
FC/FACS, IF, WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Flow Cytometry (FC/FACS) (Flow cytometric analysis of Hela cells using Mouse Mapk3 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9201597 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

Mapk3, Polyclonal Antibody (Cat# AAA9201597)

Full Name
Mouse Mapk3 Antibody (C-term)
Gene Names
Mapk3; p44; Erk1; Ert2; Mnk1; Erk-1; Esrk1; Prkm3; Mtap2k; p44erk1; p44mapk
Reactivity
Mouse
Applications
FC/FACS, EIA, IHC, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
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