Rabbit TLR7 Polyclonal Antibody | anti-TLR7 antibody

Rabbit Polyclonal (IgG) to Human TLR7

Cat. Num. AAA247379

• Gene Names: TLR7; TLR7-like
• Reactivity: Chimpanzee, Human, Monkey
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein G Purified
• Synonyms: TLR7; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human TLR7; Anti-TLR7 Antibody (aa900-950) IHC-plus; Toll-like receptor 7; Human TLR7; anti-TLR7 antibody

• Host: Rabbit
• Reactivity: Chimpanzee, Human, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: A portion of amino acids 900-950 of human TLR7
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.2% gelatin, 0.05% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1049

• Applicable Applications for anti-TLR7 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo, IHC-P (10 ug/ml), WB (0.5 - 2 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human TLR7.
• Immunogen Type: Synthetic peptide
• Immunogen: TLR7 antibody was raised against synthetic peptide from human TLR7.
• Antigen Modification: aa900-950
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-TLR7 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-TLR7 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Flow Cytometry (FC/FACS)

(Intracellular staining by FACS analysis of TLR7 in human PBMCs using at2 ug/ml. Shaded histogram represents cells alone; green represents rabbit IgG isotype control, ; red represents anti-TLR7 antibody. Goat anti-rabbit PE, was used as secondary.)

Western Blot (WB)

(Western blot of human TLR7 in Ramos cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at 1 ug/ml.)

Related Product Information for anti-TLR7 antibody

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors.

Product Categories/Family for anti-TLR7 antibody

Family: Toll-like Receptor

NCBI and Uniprot Product Information

• NCBI GI #: 7706093
• NCBI GeneID: 51284
• NCBI Accession #: NP_057646.1
• NCBI GenBank Nucleotide #: NM_016562.3
• UniProt Accession #: Q9NYK1; Q9NR98; D1CS69
• Molecular Weight: 120,922 Da
• NCBI Official Full Name: toll-like receptor 7
• NCBI Official Synonym Full Names: toll-like receptor 7
• NCBI Official Symbol: TLR7
• NCBI Official Synonym Symbols: TLR7-like
• NCBI Protein Information: toll-like receptor 7; toll-like receptor 7-like
• UniProt Protein Name: Toll-like receptor 7
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR7
• UniProt Entry Name: TLR7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR7: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Interacts with MYD88 via their respective TIR domains. Interacts with UNC93B1. Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; lysosome; endoplasmic reticulum; cytoplasm; integral to membrane; plasma membrane; endosome membrane; receptor complex; endosome

Molecular Function: single-stranded RNA binding; siRNA binding; transmembrane receptor activity; double-stranded RNA binding; drug binding

Biological Process: positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-6 production; microglial cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of interferon-alpha biosynthetic process; toll-like receptor 7 signaling pathway; positive regulation of chemokine production; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-8 production; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; inflammatory response; I-kappaB phosphorylation; defense response to virus; positive regulation of inflammatory response; positive regulation of interferon-gamma biosynthetic process