Western Blot (WB)
(Western blot analysis of CD33 expression in SKOV3 whole cell lysates (lane 1). CD33 at 45KD; 67KD was detected using rabbit anti-CD33 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rabbit anti-Human CD33 Polyclonal Antibody | anti-CD33 antibody
Anti-CD33 Antibody
Immunohistochemistry - Paraffin: 0.5-1ug/ml; Antigen Retrieval: By Heat
Immunohistochemistry - Frozen: 0.5-1ug/ml
Immunocytochemistry: 0.5-1ug/ml
Flow Cytometry: 1-3ug/1x106 cells
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Western Blot (WB)
(Western blot analysis of CD33 expression in SKOV3 whole cell lysates (lane 1). CD33 at 45KD; 67KD was detected using rabbit anti-CD33 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of CD33 expression in SKOV3 whole cell lysates (lane 1). CD33 at 45KD; 67KD was detected using rabbit anti-CD33 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Immunohistochemistry (IHC)
(CD33 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- CD33 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(CD33 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- CD33 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of U937 cells using anti-CD33 antibody (MBS178432).Overlay histogram showing U937 cells stained with MBS178432 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD33 Antibody (MBS178432,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of U937 cells using anti-CD33 antibody (MBS178432).Overlay histogram showing U937 cells stained with MBS178432 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD33 Antibody (MBS178432,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Brinkman-Van der Linden, E. C. M., Angata, T., Reynolds, S. A., Powell, L. D., Hedrick, S. M., Varki, A. CD33/Siglec-3 binding specificity, expression pattern, and consequences of gene deletion in mice. Molec. Cell. Biol. 23: 4199-4206, 2003.
3. Trask, B., Fertitta, A., Christensen, M., Youngblom, J., Bergmann, A., Copeland, A., de Jong, P., Mohrenweiser, H., Olsen, A., Carrano, A., Tynan, K. Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and 70 genes or DNA markers. Genomics 15: 133-145, 1993.
NCBI and Uniprot Product Information
Uniprot Description
CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: external side of plasma membrane; integral to plasma membrane; plasma membrane
Molecular Function: protein binding; receptor activity
Biological Process: cell-cell signaling; negative regulation of cell proliferation; regulation of immune response; signal transduction
Research Articles on CD33
Similar Products
Product Notes
The CD33 cd33 (Catalog #AAA178432) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD33 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD33 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin/Frozen, Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Western Blot: 0.1-0.5ug/ml Immunohistochemistry - Paraffin: 0.5-1ug/ml; Antigen Retrieval: By Heat Immunohistochemistry - Frozen: 0.5-1ug/ml Immunocytochemistry: 0.5-1ug/ml Flow Cytometry: 1-3ug/1x106 cells Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Researchers should empirically determine the suitability of the CD33 cd33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD33, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
