Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of lysates from Hela, NCI-H1299 cell line (from left to right),using RPL6 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

Rabbit anti-Human RPL6 Polyclonal Antibody | anti-RPL6 antibody

RPL6 Antibody (N-term)

Gene Names
RPL6; L6; TXREB1; SHUJUN-2; TAXREB107
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
Synonyms
RPL6; Polyclonal Antibody; RPL6 Antibody (N-term); 60S ribosomal protein L6; Neoplasm-related protein C140; Tax-responsive enhancer element-binding protein 107; TaxREB107; TXREB1; anti-RPL6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This RPL6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-35 amino acids from the N-terminal region of human RPL6.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
288
Applicable Applications for anti-RPL6 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
WB: 1:1000
FC/FACS: 1:25
IF: 1:25
IHC-P: 1:25
Antigen Source
Human
Antigen Region
2-35
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from Hela, NCI-H1299 cell line (from left to right),using RPL6 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

Western Blot (WB) (Western blot analysis of lysates from Hela, NCI-H1299 cell line (from left to right),using RPL6 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells using RPL6 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). It was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of Jurkat cells using RPL6 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). It was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

Immunofluorescence (IF)

(Fluorescent image of HeLa cells stained with RPL6 Antibody (N-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

Immunofluorescence (IF) (Fluorescent image of HeLa cells stained with RPL6 Antibody (N-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

Immunohistochemistry (IHC)-Paraffin

(Immunohistochemical analysis of paraffin-embedded H. gastric carcinoma section using RPL6 Antibody (N-term)(Cat#NA). NA was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC)-Paraffin (Immunohistochemical analysis of paraffin-embedded H. gastric carcinoma section using RPL6 Antibody (N-term)(Cat#NA). NA was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Related Product Information for anti-RPL6 antibody
Description: Purified Rabbit Polyclonal Antibody (Pab)

Background: Specifically binds to domain C of the Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
References
Zaman G.J.R.,et al.Nucleic Acids Res. 21:1673-1673(1993). Morita T.,et al.AIDS Res. Hum. Retroviruses 9:115-121(1993). Kenmochi N.,et al.J. Hum. Genet. 45:290-293(2000). Ohta K.,et al.J. Mol. Endocrinol. 12:85-92(1994). Odintsova T.I.,et al.J. Protein Chem. 22:249-258(2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32728
NCBI Official Full Name
60S ribosomal protein L6 isoform 1
NCBI Official Synonym Full Names
ribosomal protein L6
NCBI Official Symbol
RPL6
NCBI Official Synonym Symbols
L6; TXREB1; SHUJUN-2; TAXREB107
NCBI Protein Information
60S ribosomal protein L6
UniProt Protein Name
60S ribosomal protein L6
Protein Family
UniProt Gene Name
RPL6
UniProt Synonym Gene Names
TXREB1; TaxREB107
UniProt Entry Name
RL6_HUMAN

NCBI Description

This gene encodes a protein component of the 60S ribosomal subunit. This protein can bind specifically to domain C of the tax-responsive enhancer element of human T-cell leukemia virus type 1, and may participate in tax-mediated transactivation of transcription. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Research Articles on RPL6

Similar Products

Product Notes

The RPL6 rpl6 (Catalog #AAA9231604) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPL6 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPL6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA). WB: 1:1000 FC/FACS: 1:25 IF: 1:25 IHC-P: 1:25. Researchers should empirically determine the suitability of the RPL6 rpl6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPL6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.