Rabbit Laminin gamma-3 Polyclonal Antibody | anti-T1385 antibody

Laminin gamma-3 (T1385) Polyclonal Antibody

Cat. Num. AAA3013622

• Gene Names: LAMC3; OCCM
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Laminin gamma-3; Polyclonal Antibody; Laminin gamma-3 (T1385) Polyclonal Antibody; Laminin subunit gamma-3; Laminin-12 subunit gamma; Laminin-14 subunit gamma; Laminin-15 subunit gamma; LAMC3; Laminin gamma3; anti-T1385 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Laminin gamma-3 (T1385) polyclonal antibody detects endogenous levels of Laminin gamma-3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1575

• Applicable Applications for anti-T1385 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 1355-1399 of Human Laminin gamma-3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Laminin gamma-3 (T1385) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-T1385 antibody

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of laminins with stabilizing type IV collagen networks, nidogen, and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression, and tissue architecture. Each laminin is a heterotrimer of alpha, beta, and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by Integrins, dystroglycan, and cognate laminin receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 110611156
• NCBI GeneID: 10319
• NCBI Accession #: NP_006050.3
• NCBI GenBank Nucleotide #: NP_006050.3
• UniProt Accession #: Q9Y6N6
• Molecular Weight: ~ 172kDa
• NCBI Official Full Name: laminin subunit gamma-3
• NCBI Official Synonym Full Names: laminin subunit gamma 3
• NCBI Official Symbol: LAMC3
• NCBI Official Synonym Symbols: OCCM
• NCBI Protein Information: laminin subunit gamma-3
• UniProt Protein Name: Laminin subunit gamma-3
• UniProt Gene Name: LAMC3
• UniProt Entry Name: LAMC3_HUMAN

NCBI Description

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 3. The gamma 3 chain is most similar to the gamma 1 chain, and contains all the 6 domains expected of the gamma chain. It is a component of laminin 12. The gamma 3 chain is broadly expressed in skin, heart, lung, and the reproductive tracts. In skin, it is seen within the basement membrane of the dermal-epidermal junction at points of nerve penetration. Gamma 3 is also a prominent element of the apical surface of ciliated epithelial cells of lung, oviduct, epididymis, ductus deferens, and seminiferous tubules. The distribution of gamma 3-containing laminins along ciliated epithelial surfaces suggests that the apical laminins are important in the morphogenesis and structural stability of the ciliated processes of these cells. [provided by RefSeq, Aug 2011]

Uniprot Description

LAMC3: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Defects in LAMC3 are the cause of cortical malformations occipital (OCCM). OCCM is a disease in which affected individuals develop seizures, sometimes associated with transient visual changes. Brain MRI shows both pachygyria and polymicrogyria restricted to the lateral occipital lobes.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 9q34.12

Cellular Component: proteinaceous extracellular matrix; membrane; extracellular region; basement membrane

Molecular Function: structural molecule activity

Biological Process: cellular morphogenesis during differentiation; extracellular matrix organization and biogenesis; visual perception; retina development in camera-type eye; cell adhesion; astrocyte development

Disease: Cortical Malformations, Occipital