Rabbit anti-Human MICA Polyclonal Antibody | anti-MICA antibody

MICA Antibody (Center)

Cat. Num. AAA9206685

• Gene Names: MICA; MIC-A; PERB11.1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MICA; Polyclonal Antibody; MICA Antibody (Center); MHC class I polypeptide-related sequence A; MIC-A; MICA {ECO:0000312|EMBL:CAI419071}; anti-MICA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MICA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the Central region of human MICA.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.42 (varies by lot)
• Sequence Positions: 68-97
• Sequence Length: 383

• Applicable Applications for anti-MICA antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-MICA Antibody (Center) at 1:2000 dilutionLane 1: A431 whole cell lysatesLane 2: U-87 MG whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-MICA Antibody (Center)at 1:2000 dilution + U-87 MG whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-MICA Antibody (Center) at 1:2000 dilutionLane 1: U-87 MG whole cell lysatesLane 2: A431 whole cell lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-MICA Antibody (Center)at 1:2000 dilution + A431 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-MICA Antibody (Center) at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: Daudi whole cell lysatesLane 3: A431 whole cell lysatesLane 4: MCF-7 whole cell lysatesLane 5: U-87MG whole cell lysatesLane 6: THP-1 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-MICA Antibody (Center)at 1:1000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Flow cytometric analysis of SK-BR-3 cells using MICA Antibody (Center) (green) compared to an isotype control of rabbit IgG(blue). MBS9206685 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

Related Product Information for anti-MICA antibody

MICA is the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

Product Categories/Family for anti-MICA antibody

Immunology

References

Bahram,S., et.al., Proc. Natl. Acad. Sci. U.S.A. 91 (14), 6259-6263 (1994) Klein,J.et.al., Proc. Natl. Acad. Sci. U.S.A. 91 (14), 6251-6252 (1994) Parham,P., et.al., J. Immunol. 142 (11), 3937-3950 (1989)

NCBI and Uniprot Product Information

• NCBI GI #: 4557751
• NCBI GeneID: 100507436
• NCBI Accession #: NP_000238.1
• NCBI GenBank Nucleotide #: NM_000247.2
• UniProt Accession #: Q29983; O60917; O60918; O60919; O60920; O60933; O77956; O78150; A8KQR0; A9LRW5; A9QVE0; B1VJF1
• Molecular Weight: 42915
• NCBI Official Full Name: MHC class I polypeptide-related sequence A isoform 1 (MICA*001)
• NCBI Official Synonym Full Names: MHC class I polypeptide-related sequence A
• NCBI Official Symbol: MICA
• NCBI Official Synonym Symbols: MIC-A; PERB11.1
• NCBI Protein Information: MHC class I polypeptide-related sequence A
• UniProt Protein Name: MHC class I polypeptide-related sequence A
• Protein Family: MICAL-like protein
• UniProt Gene Name: MICA
• UniProt Synonym Gene Names: MIC-A
• UniProt Entry Name: MICA_HUMAN

NCBI Description

This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

MICA: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T- cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma. Genetic variations in MICA may be a cause of susceptibility to psoriasis type 1 (PSORS1). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Genetic variation in MICA is a cause of susceptibility to psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the MHC class I family. MIC subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: extracellular space; cell surface; integral to plasma membrane; cytoplasm

Molecular Function: protein binding; beta-2-microglobulin binding; antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; viral reproduction; response to heat; natural killer cell mediated cytotoxicity; cytolysis; defense response to bacterium; immune response to tumor cell; gamma-delta T cell activation; response to DNA damage stimulus; defense response to virus; T cell mediated cytotoxicity

Disease: Psoriatic Arthritis, Susceptibility To; Psoriasis 1, Susceptibility To