Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using Mouse Mapk3 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9201597 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Rabbit anti-Mouse Mapk3 Polyclonal Antibody | anti-Mapk3 antibody
Mouse Mapk3 Antibody (C-term)
WB~~1:1000
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using Mouse Mapk3 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9201597 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using Mouse Mapk3 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9201597 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of lysates from mouse BA/F3, C2C12, NIH/3T3 and rat C6 cell line (from left to right), using Mouse Mapk3 Antibody (C-term). MBS9201597 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from mouse BA/F3, C2C12, NIH/3T3 and rat C6 cell line (from left to right), using Mouse Mapk3 Antibody (C-term). MBS9201597 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Immunohistochemistry (IHC)
(Mouse Mapk3 Antibody (C-term) (MBS9201597)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Mouse Mapk3 Antibody (C-term) (MBS9201597)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Chandrakesan, P., et al. J. Biol. Chem. 285(43):33485-33498(2010)
Alter, B.J., et al. J. Neurosci. 30(34):11537-11547(2010)
Spruce, T., et al. Dev. Cell 19(2):207-219(2010)
Bremer, J., et al. PLoS ONE 5 (8), E12450 (2010) :
NCBI and Uniprot Product Information
Uniprot Description
ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.
Protein type: Kinase, protein; Protein kinase, CMGC; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily
Cellular Component: Golgi apparatus; focal adhesion; protein complex; mitochondrion; early endosome; nuclear envelope; caveola; pseudopodium; cytosol; nucleoplasm; microtubule cytoskeleton; cytoskeleton; late endosome; cytoplasm; nucleus
Molecular Function: transferase activity; MAP kinase activity; protein serine/threonine kinase activity; protein binding; phosphotyrosine binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity; phosphatase binding
Biological Process: positive regulation of histone phosphorylation; positive regulation of translation; DNA damage induced protein phosphorylation; apoptosis; response to toxin; response to lipopolysaccharide; sensory perception of pain; signal transduction; protein amino acid phosphorylation; BMP signaling pathway; response to exogenous dsRNA; regulation of transcription factor activity; lipopolysaccharide-mediated signaling pathway; protein complex assembly; transcription, DNA-dependent; MAPKKK cascade; cell cycle; regulation of stress-activated MAPK cascade; nuclear translocation of MAPK; peptidyl-serine phosphorylation; organ morphogenesis; cartilage development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; positive regulation of protein amino acid phosphorylation; response to DNA damage stimulus; phosphorylation; regulation of cytoskeleton organization and biogenesis
Research Articles on Mapk3
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Product Notes
The Mapk3 mapk3 (Catalog #AAA9201597) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 353-380. The Mouse Mapk3 Antibody (C-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Mapk3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). FC~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the Mapk3 mapk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mapk3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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