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538 results for " Caspases" - showing 100-150
Western Blot (WB)
(Western blot Vascular endothelial growth factor C Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 46 kDaObserved band size: 20 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Vascular endothelial growth factor C, Polyclonal Antibody (Cat# AAA715961)
Full Name
Rabbit anti-human Vascular endothelial growth factor C polyclonal Antibody, FITC
Gene Names
VEGFC; VRP; Flt4-L; LMPH1D
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data #1
(Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)
CASPASE-8, Assay Kit (Cat# AAA239195)
Full Name
FAM FLICA CASPASE-8 KIT
Gene Names
CASP8; caspase-8
Applications
FC/FACS, IF
Pricing
$1,070/100 Tests | $4,950/5x100 Tests
Testing Data
Caspase-3, Monoclonal Antibody (Cat# AAA670234)
Full Name
Mouse Anti-Human Caspase-3-HRP
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human
Applications
Western Blot
Pricing
$270/1 mL | $1,070/5x1 mL
Testing Data #1
(Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)
CASPASE-6, Assay Kit (Cat# AAA239192)
Full Name
FAM FLICA CASPASE-6 KIT
Gene Names
casp6; mch2; caspase-6; xCaspase-6
Applications
FC/FACS, IF
Pricing
$440/25 Tests | $2,005/5x25 Tests
Western Blot (WB)
(Western blot All lanes: Rho guanine nucleotide exchange factor 18 antibody at at 2 ug/ml Lane 1: EC109whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 129 kDa Observed band size: 30 kDa Additional bands at: 60kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Rho guanine nucleotide exchange factor 18, Polyclonal Antibody (Cat# AAA715672)
Full Name
Rabbit anti-human Rho guanine nucleotide exchange factor 18 polyclonal Antibody, HRP conjugated
Gene Names
ARHGEF18; P114-RhoGEF
Reactivity
Human. Other species are not tested. Please decide the specificity by homology.
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot V-type proton ATPase subunit G 1 antibody at at 2 /ml+ 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13 kDa Observed band size: 50kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
V-type proton ATPase subunit G 1, Polyclonal Antibody (Cat# AAA715464)
Full Name
Rabbit anti-human V-type proton ATPase subunit G 1 polyclonal Antibody, HRP conjugated
Gene Names
ATP6V1G1; ATP6G; ATP6J; Vma10; ATP6G1; ATP6GL
Reactivity
Human, Mouse, Rat, Cow
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Interferon-induced transmembrane protein 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 14 kDa Observed band size: 70 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This)
Interferon-induced transmembrane protein 1, Polyclonal Antibody (Cat# AAA715877)
Full Name
Rabbit anti-human Interferon-induced transmembrane protein 1 polyclonal Antibody, HRP conjugated
Gene Names
IFITM1; 9-27; CD225; IFI17; LEU13; DSPA2a
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Cytochrome b-c1 complex subunit 8 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 9.5 kDa Observed band size: 30kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Cytochrome b-c1 complex subunit 8, Polyclonal Antibody (Cat# AAA715302)
Full Name
Rabbit anti-human Cytochrome b-c1 complex subunit 8 polyclonal Antibody, FITC
Gene Names
UQCRQ; QPC; QCR8; QP-C; UQCR7; MC3DN4
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data #1
(Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)
POLY CASPASE, Assay Kit (Cat# AAA239189)
Full Name
FAM FLICA POLY CASPASE KIT
Applications
FC/FACS, IF
Pricing
$1,070/100 Tests | $4,950/5x100 Tests
Western Blot (WB)
(Western blot All lanes: Casein kinase II subunit beta antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13 kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Gamma-aminobutyric acid receptor-associated protein, Polyclonal Antibody (Cat# AAA715371)
Full Name
Rabbit anti-human Gamma-aminobutyric acid receptor-associated protein polyclonal Antibody, HRP conjugated
Gene Names
GABARAP; MM46; ATG8A; GABARAP-a
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Histone H2B type 1-C/E/F/G/I antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 14 kDa Observed band size: 70kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Histone H2B type 1-C/E/F/G/I, Polyclonal Antibody (Cat# AAA715496)
Full Name
Rabbit anti-human Histone H2B type 1-C/E/F/G/I polyclonal Antibody, FITC
Gene Names
HIST1H2BG; H2B/a; H2BFA; H2B.1A; dJ221C16.8
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Metalloproteinase inhibitor 2 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 24kDa Observed band size: 60kDa Additional bands at: 118 kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Metalloproteinase inhibitor 2, Polyclonal Antibody (Cat# AAA715584)
Full Name
Rabbit anti-human Metalloproteinase inhibitor 2 polyclonal Antibody, Biotin conjugated
Gene Names
TIMP2; DDC8; CSC-21K
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot Actin-related protein 2/3 complex subunit 3Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 20 kDaObserved band size: 75 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Actin-related protein 2/3 complex subunit 3, Polyclonal Antibody (Cat# AAA715899)
Full Name
Rabbit anti-human Actin-related protein 2/3 complex subunit 3 polyclonal Antibody, FITC
Gene Names
ARPC3; ARC21; p21-Arc
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of cIAP-2 in Jurkat cell lysates.)
cIAP2, Polyclonal Antibody (Cat# AAA837655)
Full Name
cIAP2 antibody
Applications
WB
Purity
cIAP2 antibody was purified by affinity chromatography
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: HCLS1-associated protein X-1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 31 kDa Observed band size: 40kDa Additional bands at: 90 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
HCLS1-associated protein X-1, Polyclonal Antibody (Cat# AAA715860)
Full Name
Rabbit anti-human HCLS1-associated protein X-1 polyclonal Antibody, HRP conjugated
Gene Names
HAX1; SCN3; HS1BP1; HCLSBP1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot C-C motif chemokine 7 Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 11 kDaObserved band size: 48kDa Additional bands at: 90kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
C-C motif chemokine 7, Polyclonal Antibody (Cat# AAA715774)
Full Name
Rabbit anti-human C-C motif chemokine 7 polyclonal Antibody, HRP conjugated
Gene Names
CCL7; FIC; MARC; MCP3; NC28; MCP-3; SCYA6; SCYA7
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Metalloproteinase inhibitor 2 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 24kDa Observed band size: 60kDa Additional bands at: 118 kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Metalloproteinase inhibitor 2, Polyclonal Antibody (Cat# AAA715811)
Full Name
Rabbit anti-human Metalloproteinase inhibitor 2 polyclonal Antibody, HRP conjugated
Gene Names
TIMP2; DDC8; CSC-21K
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Tax1-binding protein 3 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13kDa Observed band size: 70 kDa Additional bands at: 36 kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Tax1-binding protein 3, Polyclonal Antibody (Cat# AAA715725)
Full Name
Rabbit anti-human Tax1-binding protein 3 polyclonal Antibody, Biotin conjugated
Gene Names
TAX1BP3; TIP-1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data
(Jurkat cells were treated withstaurosporine, an apoptosis-inducingagent (bottom), or DMSO, a negativecontrol (top), for 4 hours, thenincubated with ICT's green caspase 10inhibitor probe, FAM-AEVD-FMK, for 1hour. Cells were washed twice and readon a flow cytometer. Treatment withstaurosporine induced caspase 10activity in 93.6% of the experimentalcells (M2, bottom right), whereas thenegative control treatment exhibitedcaspase 10 activity in only 4.7% of thecell population (M2, top right). This is a ratio of 20:1. (Dr. Brian W. Lee, ICT).)
FAM-FLICA Caspase 10, Assay Kit (Cat# AAA258025)
Full Name
FAM-FLICA Caspase 10 Assay Kit
Pricing
$665/100 Tests | $300/25 Tests | $3,010/5x100 Tests
Western Blot (WB)
(Western blot V-type proton ATPase subunit G 1 antibody at at 2 /ml+ 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13 kDa Observed band size: 50kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
V-type proton ATPase subunit G 1, Polyclonal Antibody (Cat# AAA715382)
Full Name
Rabbit anti-human V-type proton ATPase subunit G 1 polyclonal Antibody, Biotin conjugated
Gene Names
ATP6V1G1; ATP6G; ATP6J; Vma10; ATP6G1; ATP6GL
Reactivity
Human, Mouse, Rat, Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of cIAP-1 inmouse small intestine tissue lysate.)
cIAP1, Polyclonal Antibody (Cat# AAA835448)
Full Name
cIAP1 antibody
Applications
WB
Purity
cIAP1 antibody was purified by affinity chromatography
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Peptidyl-prolyl cis-trans isomerase FKBP1A antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 12 kDa Observed band size: 22 kDa Additional bands at: 36 kDa. We are unsure as to the identity of these extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Peptidyl-prolyl cis-trans isomerase FKBP1A, Polyclonal Antibody (Cat# AAA715062)
Full Name
Rabbit anti-human Peptidyl-prolyl cis-trans isomerase FKBP1A polyclonal Antibody, FITC
Gene Names
FKBP1A; FKBP1; PKC12; PKCI2; FKBP12; PPIASE; FKBP-12; FKBP-1A
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Tumor necrosis factor antibody at 2/mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 26 kDa Observed band size: 60 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Tumor necrosis factor, Polyclonal Antibody (Cat# AAA719434)
Full Name
Rabbit anti-Bovine Tumor necrosis factor polyclonal Antibody, FITC
Gene Names
TNF; TNFa; TNF-a; TNF-alpha
Reactivity
Bovine
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
FasL, Polyclonal Antibody (Cat# AAA535867)
Full Name
FasL antibody
Reactivity
Human FasL
Applications
EIA, WB
Pricing
$425/0.05 mg | $1,905/5x0.05 mg
Western Blot (WB)
(Western blot Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Antibody at 2 ug/ml + EC109 whole cell lysate at 20 ug Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 34 kDa Observed band size: 45 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, Polyclonal Antibody (Cat# AAA715317)
Full Name
Rabbit anti-human Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform polyclonal Antibody, FITC
Gene Names
PPP2CB; PP2CB; PP2Abeta
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data #1
(Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)
CASPASE-3/7, Assay Kit (Cat# AAA239216)
Full Name
FAM FLICA CASPASE-3/7 KIT
Applications
FC/FACS, IF
Pricing
$440/25 Tests | $2,005/5x25 Tests
Western Blot (WB)
(Western blot All lanes: Activator of 90 kDa heat shock protein ATPase homolog 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 37 kDa Observed band size: 45kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) · multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Activator of 90 kDa heat shock protein ATPase homolog 1, Polyclonal Antibody (Cat# AAA715985)
Full Name
Rabbit anti-human Activator of 90 kDa heat shock protein ATPase homolog 1 polyclonal Antibody, HRP conjugated
Gene Names
AHSA1; p38; AHA1; C14orf3
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western analysis of extracts from Jurkat (human), NIH3T3 (mouse) or C6 (rat) cells, untreated, etoposide-treated (25uM in vivo) or cytochrome c-treated, (0.25mg/ml in vitro), using MBS631626)
Caspase 3, Cleaved, Monoclonal Antibody (Cat# AAA631626)
Full Name
Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1)
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB, IP, IHC, ICC, FC/FACS, IF
Purity
Supernatant
Supernatant
Supernatant
Pricing
$670/0.1 mL | $3,010/5x0.1 mL
LYN, Polyclonal Antibody (Cat# AAA534188)
Full Name
LYN antibody
Reactivity
To be determined by end-user
Applications
User optimized
Pricing
$350/0.25 mg | $1,570/5x0.25 mg
Testing Data #1
(Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)
POLY CASPASE, Assay Kit (Cat# AAA239204)
Full Name
FAM FLICA POLY CASPASE KIT
Applications
FC/FACS, IF
Pricing
$440/25 Tests | $2,005/5x25 Tests
Caspase 3, Recombinant Protein (Cat# AAA5303832)
Full Name
Caspase 3 protein
Gene Names
Casp3; caspase-3
Purity
Caspase 3 protein was purified using a a proprietary chromatographic technique.
Pricing
$540/100 Units | $2,425/5x100 Units
DIABLO, Polyclonal Antibody (Cat# AAA536104)
Full Name
DIABLO antibody
Gene Names
DIABLO; SMAC; DFNA64
Reactivity
Human
Applications
WB
Purity
DIABLO antibody was purified by affinity chromatography.
Pricing
$385/0.1 mg | $1,730/5x0.1 mg
Caspase 9, p35, Polyclonal Antibody (Cat# AAA614593)
Full Name
Anti - Caspase 9, p35 (ICE-LAP6, Mch6, Apaf 3)
Gene Names
CASP9; MCH6; APAF3; APAF-3; ICE-LAP6; CASPASE-9c
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Serum
Pricing
$755/0.1 mL | $3,395/5x0.1 mL
Testing Data
Caspase-8, Monoclonal Antibody (Cat# AAA670338)
Full Name
Rat Anti-Human Caspase-8-UNLB
Gene Names
CASP8; caspase-8
Reactivity
Human
Applications
Western Blot
Pricing
$280/0.1 mg | $1,110/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Activator of 90 kDa heat shock protein ATPase homolog 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 37 kDa Observed band size: 45kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) · multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Activator of 90 kDa heat shock protein ATPase homolog 1, Polyclonal Antibody (Cat# AAA716033)
Full Name
Rabbit anti-human Activator of 90 kDa heat shock protein ATPase homolog 1 polyclonal Antibody, Biotin conjugated
Gene Names
AHSA1; p38; AHA1; C14orf3
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Caspase 3, Recombinant Protein (Cat# AAA5304184)
Full Name
Caspase 3 protein
Gene Names
Casp3; caspase-3
Purity
Caspase 3 protein was purified using a a proprietary chromatographic technique.
Pricing
$550/100 Units | $2,465/5x100 Units
Caspase 10, Polyclonal Antibody (Cat# AAA536248)
Full Name
Caspase 10 antibody
Gene Names
CASP10; MCH4; ALPS2; FLICE2
Reactivity
Human
Applications
WB
Purity
Caspase 10 antibody was purified by affinity chromatography.
Pricing
$385/0.1 mg | $1,730/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Spliceosome RNA helicase DDX39B antibody at 2 ug/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 47 kDa Observed band size: 40kDa Additional bands at: 60 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Spliceosome RNA helicase DDX39B, Polyclonal Antibody (Cat# AAA719922)
Full Name
Rabbit anti-human Spliceosome RNA helicase DDX39B polyclonal Antibody, HRP conjugated
Gene Names
DDX39B; BAT1; UAP56; D6S81E
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot Actin-related protein 2/3 complex subunit 3Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 20 kDaObserved band size: 75 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Actin-related protein 2/3 complex subunit 3, Polyclonal Antibody (Cat# AAA715765)
Full Name
Rabbit anti-human Actin-related protein 2/3 complex subunit 3 polyclonal Antibody, HRP conjugated
Gene Names
ARPC3; ARC21; p21-Arc
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Caspase 3, Recombinant Protein (Cat# AAA5304552)
Full Name
Caspase 3 protein
Gene Names
Casp3; caspase-3
Purity
Caspase 3 protein was purified using a a proprietary chromatographic technique.
Pricing
$525/100 Units | $2,350/5x100 Units
Western Blot (WB)
(Western blot All lanes: Nucleoside diphosphate kinase A antibody at at 2 /mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size:16.7 kDa Observed band size: 32kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Nucleoside diphosphate kinase A, Polyclonal Antibody (Cat# AAA715318)
Full Name
Rabbit anti-human Nucleoside diphosphate kinase A polyclonal Antibody, HRP conjugated
Gene Names
NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Protein AATF antibody at at 2 /mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 62kDa Observed band size: 50kDa Additional bands at: 100kDa?10 kDa. We are unsure as to the identity of these extra bands. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Protein AATF, Polyclonal Antibody (Cat# AAA715793)
Full Name
Rabbit anti-human Protein AATF polyclonal Antibody, HRP conjugated
Gene Names
AATF; DED; BFR2; CHE1; CHE-1
Reactivity
Human, Mouse
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data
(Jurkat cells were treated with 1uM staurosporine to induce caspaseactivity (top), or a negative control (bottom) for 3 hours, incubatedwith ICT's red poly caspases inhibitor probe, SR-VAD-FMK, for 1 hour,washed twice, and examined under a fluorescence microscope (DICimages were also taken). The color image of induced cells (upper left)reveals experimental cells which fluoresce red, therefore they all havesome degree of caspase activity. The non-induced DIC image (lowerright) reveals many control cells, however the correspondingfluorescence image (lower left) is dark; none of these cells have activecaspases (Dr. Brian W. Lee, ICT).)
SR-FLICA Poly Caspase, Assay Kit (Cat# AAA258012)
Full Name
SR-FLICA Poly Caspase Assay Kit
Pricing
$665/100 Tests | $300/25 Tests | $3,010/5x100 Tests
Western Blot (WB)
(Western blot All lanes: DNA-directed RNA polymerases I, II, and III subunit RPABC2 antibody at at 2 ug/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 49 kDa Observed band size: 36kDa Additional bands at: 80 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Heterogeneous nuclear ribonucleoprotein H, Polyclonal Antibody (Cat# AAA715925)
Full Name
Rabbit anti-human Heterogeneous nuclear ribonucleoprotein H polyclonal Antibody, FITC
Gene Names
HNRNPH1; HNRPH; HNRPH1; hnRNPH
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Lane 1: Recombinant Human TNFR1 Protein 10ng; Lane 2: Recombinant Human TNFR1 Protein 5ng; Lane 3: Recombinant Human TNFR1 Protein 2.5ng)
TNFR1, Polyclonal Antibody (Cat# AAA5301805)
Full Name
TNFR1 antibody
Applications
WB, IHC
Purity
TNFR1 antibody was purified by affinity chromatography
Pricing
$625/0.1 mg | $2,800/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Rho guanine nucleotide exchange factor 18 antibody at at 2 ug/ml Lane 1: EC109whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 129 kDa Observed band size: 30 kDa Additional bands at: 60kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Rho guanine nucleotide exchange factor 18, Polyclonal Antibody (Cat# AAA715731)
Full Name
Rabbit anti-human Rho guanine nucleotide exchange factor 18 polyclonal Antibody, FITC
Gene Names
ARHGEF18; P114-RhoGEF
Reactivity
Human. Other species are not tested. Please decide the specificity by homology.
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Protein canopy homolog 2 antibody at at 2 /ml+293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 20 kDa Observed band size: 47kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Protein canopy homolog 2, Polyclonal Antibody (Cat# AAA1265081)
Full Name
Rabbit anti-human Protein canopy homolog 2 polyclonal Antibody, HRP conjugated
Gene Names
CNPY2; MSAP; TMEM4; ZSIG9; HP10390
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 11.7 kDa Observed band size: 40 kDa Additional bands at: 80kDa. We are unsure as to the identity of these extra bands. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
NADH dehydrogenase [ubiquinone] iron-sulfur protein 5, Polyclonal Antibody (Cat# AAA715361)
Full Name
Rabbit anti-human NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 polyclonal Antibody, FITC
Gene Names
NDUFS5; CI15K; CI-15k
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot 60S ribosomal protein L36a-like Antibody at 2 ug/ml + 293T whole cell lysate at 20 ug Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 12 kDa Observed band size:20kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
60S ribosomal protein L36a, Polyclonal Antibody (Cat# AAA715428)
Full Name
Rabbit anti-human 60S ribosomal protein L36a-like polyclonal Antibody, FITC
Gene Names
RPL36AL; RPL36A
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Interferon gamma ntibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 18.3 kDaObserved band size: 27 kDa Additional bands at: 70kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Interferon gamma, Polyclonal Antibody (Cat# AAA715994)
Full Name
Rabbit anti-human Interferon gamma polyclonal Antibody, Biotin conjugated
Gene Names
IFNG; IFG; IFI
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
