Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
538 results for " Caspases" - showing 50-100
Western Blot (WB)
(Western blot All lanes: Phosphatidylserine synthase 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 52 kDa Observed band size: 36kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Phosphatidylserine synthase 1, Polyclonal Antibody (Cat# AAA715841)
Full Name
Rabbit anti-human Phosphatidylserine synthase 1 polyclonal Antibody, HRP conjugated
Gene Names
PTDSS1; LMHD; PSS1; PSSA
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot 60S ribosomal protein L36a-like Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 12 kDaObserved band size:20kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
60S ribosomal protein L36a, Polyclonal Antibody (Cat# AAA715420)
Full Name
60S ribosomal protein L36a
Gene Names
RPL36AL; RPL36A
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
CCHL, Monoclonal Antibody (Cat# AAA832277)
Full Name
CCHL antibody
Gene Names
HCCS; MLS; CCHL; MCOPS7; LSDMCA1
Applications
WB
Purity
CCHL antibody was purified by affinity chromatography
Pricing
$590/0.1 mg | $2,650/5x0.1 mg
Western Blot (WB)
(Western blot Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Antibody at 2 /ml + EC109 whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 34 kDaObserved band size: 45 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, Polyclonal Antibody (Cat# AAA715027)
Full Name
Rabbit anti-human Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform polyclonal Antibody, Biotin conjugated
Gene Names
PPP2CB; PP2CB; PP2Abeta
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Nucleoside diphosphate kinase A antibody at at 2 /mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size:16.7 kDa Observed band size: 32kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Nucleoside diphosphate kinase A, Polyclonal Antibody (Cat# AAA715264)
Full Name
Rabbit anti-human Nucleoside diphosphate kinase A polyclonal Antibody, FITC
Gene Names
NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data
(FLUORESCENCE PROPERTIES OF FLICA 660POLY CASPASE REAGENT:Excitation max:660 nm Emission max: 690nm)
FLICA 660 Poly Caspase, Assay Kit (Cat# AAA258017)
Full Name
FLICA 660 Poly Caspase Assay Kit
Pricing
$335/25-50 Tests
Western Blot (WB)
(Western blot All lanes: AP-2 complex subunit mu antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 48 kDa Observed band size: 22kDa Additional bands at: 88 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
AP-2 complex subunit mu, Polyclonal Antibody (Cat# AAA715344)
Full Name
Rabbit anti-human AP-2 complex subunit mu polyclonal Antibody, Biotin conjugated
Gene Names
AP2M1; mu2; AP50; CLAPM1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Interferon-induced transmembrane protein 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 14 kDa Observed band size: 70 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Interferon-induced transmembrane protein 1, Polyclonal Antibody (Cat# AAA715747)
Full Name
Rabbit anti-human Interferon-induced transmembrane protein 1 polyclonal Antibody, FITC
Gene Names
IFITM1; 9-27; CD225; IFI17; LEU13; DSPA2a
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: UV excision repair protein RAD23 homolog A antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 21 kDa Observed band size: 36kDa Additional bands at: 60 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Programmed cell death protein 6, Polyclonal Antibody (Cat# AAA715734)
Full Name
Rabbit anti-human Programmed cell death protein 6 polyclonal Antibody, Biotin conjugated
Gene Names
PDCD6; ALG2; ALG-2; PEF1B
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data #1
(Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)
CASPASE-2, Assay Kit (Cat# AAA239214)
Full Name
FAM FLICA CASPASE-2 KIT
Gene Names
Casp2; ICH-1; Nedd2; Caspase-2
Applications
FC/FACS, IF
Pricing
$440/25 Tests | $2,005/5x25 Tests
Western Blot (WB)
(Western blot All lanes: Cytochrome b-c1 complex subunit 10 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 6.4kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Cytochrome b-c1 complex subunit 10, Polyclonal Antibody (Cat# AAA715423)
Full Name
Rabbit anti- human Cytochrome b-c1 complex subunit 10 polyclonal Antibody, FITC
Gene Names
UQCR11; UQCR; QCR10; 0710008D09Rik
Reactivity
Human
Applications
EIA
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: 60S ribosomal protein L15 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 22.4kDa Observed band size: 70 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
60S ribosomal protein L15, Polyclonal Antibody (Cat# AAA715886)
Full Name
Rabbit anti-human 60S ribosomal protein L15 polyclonal Antibody, Biotin conjugated
Gene Names
RPL15; L15; EC45; DBA12; RPL10; RPLY10; RPYL10
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Charged multivesicular body protein 2a antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 24 kDa Observed band size: 16kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Charged multivesicular body protein 2a, Polyclonal Antibody (Cat# AAA715778)
Full Name
Rabbit anti-human Charged multivesicular body protein 2a polyclonal Antibody, FITC
Gene Names
CHMP2A; BC2; BC-2; VPS2; CHMP2; VPS2A
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: HCLS1-associated protein X-1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 31 kDa Observed band size: 40kDa Additional bands at: 90 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
HCLS1-associated protein X-1, Polyclonal Antibody (Cat# AAA715815)
Full Name
Rabbit anti-human HCLS1-associated protein X-1 polyclonal Antibody, Biotin conjugated
Gene Names
HAX1; SCN3; HS1BP1; HCLSBP1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
FAM-FLICA Caspase 1, Assay Kit (Cat# AAA258406)
Full Name
FAM-FLICA Caspase 1 Assay Kit
Applications
Flow Cytometer, Fluorescence Microscope, Fluorescence Plate Reader
Pricing
$695/100 Tests | $315/25 Tests | $3,140/5x100 Tests
Western Blot (WB)
(Western blot All lanes: Phosphatidylserine synthase 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 52 kDa Observed band size: 36kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Phosphatidylserine synthase 1, Polyclonal Antibody (Cat# AAA715976)
Full Name
Rabbit anti-human Phosphatidylserine synthase 1 polyclonal Antibody, Biotin conjugated
Gene Names
PTDSS1; LMHD; PSS1; PSSA
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data #1
(Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)
CASPASE-9, Assay Kit (Cat# AAA239219)
Full Name
FAM FLICA CASPASE-9 KIT
Gene Names
CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
Applications
FC/FACS, IF
Pricing
$440/25 Tests | $2,005/5x25 Tests
Western Blot (WB)
(Recommended TNFRSF10A Antibody)
TNFRSF10A, Polyclonal Antibody (Cat# AAA833257)
Full Name
TNFRSF10A antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Applications
WB
Purity
Affinity Purified
Pricing
$605/0.1 mL | $2,710/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BIRC7 protein (Right) or empty vector (Left) detected with BIRC7 antibody)
BIRC7, Monoclonal Antibody (Cat# AAA830736)
Full Name
BIRC7 antibody
Gene Names
BIRC7; KIAP; LIVIN; MLIAP; RNF50; ML-IAP
Applications
FC/FACS, IHC, WB
Purity
BIRC7 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB)
(Western blot All lanes: 60S ribosomal protein L15 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 22.4kDa Observed band size: 70 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
60S ribosomal protein L15, Polyclonal Antibody (Cat# AAA715952)
Full Name
Rabbit anti-human 60S ribosomal protein L15 polyclonal Antibody, FITC
Gene Names
RPL15; L15; EC45; DBA12; RPL10; RPLY10; RPYL10
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Nucleoside diphosphate kinase A antibody at at 2 /mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size:16.7 kDa Observed band size: 32kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Nucleoside diphosphate kinase A, Polyclonal Antibody (Cat# AAA715236)
Full Name
Rabbit anti-human Nucleoside diphosphate kinase A polyclonal Antibody, Biotin conjugated
Gene Names
NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Activator of 90 kDa heat shock protein ATPase homolog 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 37 kDa Observed band size: 45kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) · multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Activator of 90 kDa heat shock protein ATPase homolog 1, Polyclonal Antibody (Cat# AAA715905)
Full Name
Rabbit anti-human Activator of 90 kDa heat shock protein ATPase homolog 1 polyclonal Antibody, FITC
Gene Names
AHSA1; p38; AHA1; C14orf3
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot C-C motif chemokine 2 Antibody at 2 /ml + EC109 whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 11kDaObserved band size: 50 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
C-C motif chemokine 2, Polyclonal Antibody (Cat# AAA715447)
Full Name
Rabbit anti-human C-C motif chemokine 2 polyclonal Antibody, HRP conjugated
Gene Names
CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: UV excision repair protein RAD23 homolog A antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 21 kDa Observed band size: 36kDa Additional bands at: 60 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Programmed cell death protein 6, Polyclonal Antibody (Cat# AAA715846)
Full Name
Rabbit anti-human Programmed cell death protein 6 polyclonal Antibody, HRP conjugated
Gene Names
PDCD6; ALG2; ALG-2; PEF1B
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: UV excision repair protein RAD23 homolog A antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 21 kDa Observed band size: 36kDa Additional bands at: 60 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Programmed cell death protein 6, Polyclonal Antibody (Cat# AAA715616)
Full Name
Rabbit anti-human Programmed cell death protein 6 polyclonal Antibody, FITC
Gene Names
PDCD6; ALG2; ALG-2; PEF1B
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Peptidyl-prolyl cis-trans isomerase FKBP1A antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 12 kDa Observed band size: 22 kDa Additional bands at: 36 kDa. We are unsure as to the identity of these extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Peptidyl-prolyl cis-trans isomerase FKBP1A, Polyclonal Antibody (Cat# AAA715013)
Full Name
Rabbit anti-human Peptidyl-prolyl cis-trans isomerase FKBP1A polyclonal Antibody, Biotin conjugated
Gene Names
FKBP1A; FKBP1; PKC12; PKCI2; FKBP12; PPIASE; FKBP-12; FKBP-1A
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot C-C motif chemokine 7 Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 11 kDaObserved band size: 48kDa Additional bands at: 90kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
C-C motif chemokine 7, Polyclonal Antibody (Cat# AAA716004)
Full Name
Rabbit anti-human C-C motif chemokine 7 polyclonal Antibody, FITC
Gene Names
CCL7; FIC; MARC; MCP3; NC28; MCP-3; SCYA6; SCYA7
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot Neutrophil gelatinase-associated lipocalin Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size:22 kDaObserved band size: 45 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
NGAL, Polyclonal Antibody (Cat# AAA715084)
Full Name
Rabbit anti-human NGAL polyclonal Antibody, Biotin conjugated
Gene Names
LCN2; p25; 24p3; MSFI; NGAL
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data #1
(Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)
CASPASE-9, Assay Kit (Cat# AAA239190)
Full Name
FAM FLICA CASPASE-9 KIT
Gene Names
CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
Applications
FC/FACS, IF
Pricing
$1,070/100 Tests | $4,950/5x100 Tests
Western Blot (WB)
(Western blot All lanes: Eukaryotic translation initiation factor 3, subunit 2 beta antibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 36 kDaObserved band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Eukaryotic translation initiation factor 3 subunit I, Polyclonal Antibody (Cat# AAA715354)
Full Name
Rabbit anti-human Eukaryotic translation initiation factor 3 subunit I polyclonal Antibody, Biotin conjugated
Gene Names
EIF3I; TRIP1; EIF3S2; TRIP-1; PRO2242; eIF3-p36; eIF3-beta
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Cytochrome b-c1 complex subunit 8 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 9.5 kDa Observed band size: 30kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Cytochrome b-c1 complex subunit 8, Polyclonal Antibody (Cat# AAA715328)
Full Name
Rabbit anti-human Cytochrome b-c1 complex subunit 8 polyclonal Antibody, Biotin conjugated
Gene Names
UQCRQ; QPC; QCR8; QP-C; UQCR7; MC3DN4
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data #1
(Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)
CASPASE-10, Assay Kit (Cat# AAA239207)
Full Name
FAM FLICA CASPASE-10 KIT
Gene Names
CASP10; MCH4; ALPS2; FLICE2
Applications
FC/FACS, IF
Pricing
$1,070/100 Tests | $4,950/5x100 Tests
Western Blot (WB)
(Western blot All lanes: Interferon-induced transmembrane protein 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 14 kDa Observed band size: 70 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Interferon-induced transmembrane protein 1, Polyclonal Antibody (Cat# AAA716031)
Full Name
Rabbit anti-human Interferon-induced transmembrane protein 1 polyclonal Antibody, Biotin conjugated
Gene Names
IFITM1; 9-27; CD225; IFI17; LEU13; DSPA2a
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot C-C motif chemokine 2 Antibody at 2 /ml + EC109 whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 11kDaObserved band size: 50 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
C-C motif chemokine 2, Polyclonal Antibody (Cat# AAA715513)
Full Name
Rabbit anti-human C-C motif chemokine 2 polyclonal Antibody, FITC
Gene Names
CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Cytochrome b-c1 complex subunit 8 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 9.5 kDa Observed band size: 30kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Cytochrome b-c1 complex subunit 8, Polyclonal Antibody (Cat# AAA715270)
Full Name
Rabbit anti-human Cytochrome b-c1 complex subunit 8 polyclonal Antibody, HRP conjugated
Gene Names
UQCRQ; QPC; QCR8; QP-C; UQCR7; MC3DN4
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Caspase-3, Polyclonal Antibody (Cat# AAA395686)
Full Name
Caspase-3
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human
Applications
WB
Purity
Ammonium Sulfate Precipitation
Pricing
$390/0.1 mg | $1,610/5x0.1 mg
Western Blot (WB)
(CASP7 antibody (MBS536456) used at 0.2-1 ug/ml to detect target protein.)
CASP7, Polyclonal Antibody (Cat# AAA536456)
Full Name
CASP7 antibody
Gene Names
CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
Applications
WB
Pricing
$605/0.1 mL | $2,710/5x0.1 mL
Western Blot (WB)
(Western blot All lanes: Casein kinase II subunit beta antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13 kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Gamma-aminobutyric acid receptor-associated protein, Polyclonal Antibody (Cat# AAA715063)
Full Name
Rabbit anti-human Gamma-aminobutyric acid receptor-associated protein polyclonal Antibody, FITC
Gene Names
GABARAP; MM46; ATG8A; GABARAP-a
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of caspase-8 expression in mouse intestine and rat kidney tissue lysate)
Active Caspase 8, Polyclonal Antibody (Cat# AAA837619)
Full Name
Active Caspase 8 antibody
Applications
WB
Purity
Active Caspase 8 antibody was purified by affinity chromatography
Pricing
$495/0.1 mg | $2,215/5x0.1 mg
Western Blot (WB)
(Western blot C-X-C motif chemokine 5 Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 12.5 kDaObserved band size: 90 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
C-X-C motif chemokine 5, Polyclonal Antibody (Cat# AAA715929)
Full Name
Rabbit anti-human C-X-C motif chemokine 5 polyclonal Antibody, HRP conjugated
Gene Names
CXCL5; SCYB5; ENA-78
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data #1
(Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)
CASPASE-1, Assay Kit (Cat# AAA239178)
Full Name
FAM FLICA CASPASE-1 KIT
Gene Names
Casp1; Ice; Il1bc
Applications
FC/FACS, IF
Pricing
$470/25 Tests | $2,135/5x25 Tests
Western Blot (WB)
(Recommended CASP3 Antibody Titration: 5ug/ml)
CASP3, Polyclonal Antibody (Cat# AAA833693)
Full Name
CASP3 antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Applications
IHC, WB
Pricing
$535/0.1 mL | $2,395/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of in vitro translated p35 baculovirus protein using MBS6006015 at 1:2000. Full-length p35 is detected at ~35kD, lower molecular p35 breakdown/cleavage bands are also detected.)
p35, Baculovirus, Polyclonal Antibody (Cat# AAA6006015)
Full Name
p35, Baculovirus
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested.
Purity
Grade: Serum
Purity: Serum
Purity: Serum
Pricing
$525/0.05 mL | $2,365/5x0.05 mL
Western Blot (WB)
(Western blot All lanes: Charged multivesicular body protein 2a antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 24 kDa Observed band size: 16kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Charged multivesicular body protein 2a, Polyclonal Antibody (Cat# AAA715785)
Full Name
Rabbit anti-human Charged multivesicular body protein 2a polyclonal Antibody, HRP conjugated
Gene Names
CHMP2A; BC2; BC-2; VPS2; CHMP2; VPS2A
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Cytochrome b-c1 complex subunit 10 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 6.4kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Cytochrome b-c1 complex subunit 10, Polyclonal Antibody (Cat# AAA715210)
Full Name
Rabbit anti- human Cytochrome b-c1 complex subunit 10 polyclonal Antibody, HRP conjugated
Gene Names
UQCR11; UQCR; QCR10; 0710008D09Rik
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Protein AATF antibody at at 2 /mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 62kDa Observed band size: 50kDa Additional bands at: 100kDa?10 kDa. We are unsure as to the identity of these extra bands. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Protein AATF, Polyclonal Antibody (Cat# AAA715767)
Full Name
Rabbit anti-human Protein AATF polyclonal Antibody, Biotin conjugated
Gene Names
AATF; DED; BFR2; CHE1; CHE-1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot V-type proton ATPase subunit G 1 antibody at at 2 /ml+ 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13 kDa Observed band size: 50kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
V-type proton ATPase subunit G 1, Polyclonal Antibody (Cat# AAA715298)
Full Name
Rabbit anti-human V-type proton ATPase subunit G 1 polyclonal Antibody, FITC
Gene Names
ATP6V1G1; ATP6G; ATP6J; Vma10; ATP6G1; ATP6GL
Reactivity
Human, Mouse, Rat, Cow
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
RFI, Polyclonal Antibody (Cat# AAA222824)
Full Name
RABBIT ANTI HUMAN RFI
Gene Names
RNF34; RFI; RIF; RIFF; hRFI; CARP1; CARP-1
Reactivity
Bovine (Expected from Sequence), Dog (Expected from Sequence), Mouse (Expected from Sequence), New World primate (Expected from Sequence), Rat (Expected from Sequence)
Applications
EIA, WB
Pricing
$420/0.1 mg | $1,735/5x0.1 mg
Western Blot (WB)
(Western blot Alpha-synuclein antibody at 2 /ml+293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 16 kDa Observed band size: 55 kDa Additional bands at: 118kDa. We are unsure as to the identity of these extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Alpha-synuclein, Polyclonal Antibody (Cat# AAA719495)
Full Name
Rabbit anti-human Alpha-synuclein polyclonal Antibody, HRP conjugated
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(RFFL Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the RFFL antibody detected the RFFL protein (arrow).)
RFFL, Polyclonal Antibody (Cat# AAA6012619)
Full Name
RFFL, CT (RFFL, RNF189, RNF34L, E3 ubiquitin-protein ligase rififylin, Caspase regulator CARP2, Caspases-8 and -10-associated RING finger protein 2, FYVE-RING finger protein Sakura, RING finger and FYVE-like domain-containing protein 1, RING finger protei
Gene Names
RFFL; CARP2; FRING; CARP-2; RNF189; RNF34L; RIFIFYLIN
Reactivity
Human
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$665/0.2 mL | $2,975/5x0.2 mL
