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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
538 results for " Caspases" - showing 200-250
Western Blot (WB)
(Western blot All lanes: Biogenesis of lysosome-related organelles complex 1 subunit 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 16kDa Observed band size: 75kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Biogenesis of lysosome-related organelles complex 1 subunit 1, Polyclonal Antibody (Cat# AAA1265043)
Full Name
Rabbit anti-human Biogenesis of lysosome-related organelles complex 1 subunit 1 polyclonal Antibody, FITC
Gene Names
BLOC1S1; RT14; BLOS1; MICoA; GCN5L1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Caspase-7 (ICE, LAP3, Mch3, CHM-1), Polyclonal Antibody (Cat# AAA395418)
Full Name
Caspase-7 (ICE, LAP3, Mch3, CHM-1)
Gene Names
CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
Reactivity
Human
Applications
WB
Purity
Ammonium Sulfate Precipitation
Pricing
$390/0.1 mg | $1,610/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Spliceosome RNA helicase DDX39B antibody at 2 ug/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 47 kDa Observed band size: 40kDa Additional bands at: 60 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Spliceosome RNA helicase DDX39B, Polyclonal Antibody (Cat# AAA719540)
Full Name
Rabbit anti-human Spliceosome RNA helicase DDX39B polyclonal Antibody, FITC
Gene Names
DDX39B; BAT1; UAP56; D6S81E
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: DNA-directed RNA polymerases I, II, and III subunit RPABC2 antibody at at 2 ug/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 49 kDa Observed band size: 36kDa Additional bands at: 80 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Heterogeneous nuclear ribonucleoprotein H, Polyclonal Antibody (Cat# AAA715829)
Full Name
Rabbit anti-human Heterogeneous nuclear ribonucleoprotein H polyclonal Antibody, HRP conjugated
Gene Names
HNRNPH1; HNRPH; HNRPH1; hnRNPH
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Spliceosome RNA helicase DDX39B antibody at 2 ug/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 47 kDa Observed band size: 40kDa Additional bands at: 60 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Spliceosome RNA helicase DDX39B, Polyclonal Antibody (Cat# AAA719873)
Full Name
Rabbit anti-human Spliceosome RNA helicase DDX39B polyclonal Antibody, Biotin conjugated
Gene Names
DDX39B; BAT1; UAP56; D6S81E
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: 60S ribosomal protein L17 antibody at at 2 /ml + EC109whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 20 kDaObserved band size: 27 kDa Additional bands at: 80kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
60S ribosomal protein L17, Polyclonal Antibody (Cat# AAA715655)
Full Name
Rabbit anti- human 60S ribosomal protein L17 polyclonal Antibody, FITC
Gene Names
RPL17; L17; PD-1; RPL23
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Vesicular, overexpressed in cancer, prosurvival protein 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 19kDa Observed band size: 50kDa Additional bands at: 70kDa, 80kDa; We are unsure as to the identity of these extra bands. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Vesicular, overexpressed in cancer, prosurvival protein 1, Polyclonal Antibody (Cat# AAA715723)
Full Name
Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, FITC
Gene Names
VOPP1; ECOP; GASP
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot/Blotting
(Western blot analysis)
Caspase-7, Monoclonal Antibody (Cat# AAA395194)
Full Name
Caspase-7
Gene Names
CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
Reactivity
Human
Applications
WB
Purity
Protein A/G Chromatography
Pricing
$390/0.1 mg | $1,610/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: C-C motif chemokine 22 antibody at at 2 /mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 10 kDa Observed band size: 50 kDa Additional bands at: 80 kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
C-C motif chemokine 22, Polyclonal Antibody (Cat# AAA715559)
Full Name
Rabbit anti-human C-C motif chemokine 22 polyclonal Antibody, Biotin conjugated
Gene Names
CCL22; MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
SDS-Page
RNF34, Recombinant Protein (Cat# AAA205267)
Full Name
RNF34, 1-373aa, Human, His tag, E Coli
Gene Names
RNF34; RFI; RIF; RIFF; hRFI; CARP1; CARP-1
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
$230/0.02 mg | $405/0.1 mg | $985/0.5 mg | $4,180/5x0.5 mg
Western Blot (WB)
(Western blot C-C motif chemokine 7 Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 11 kDaObserved band size: 48kDa Additional bands at: 90kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
C-C motif chemokine 7, Polyclonal Antibody (Cat# AAA715832)
Full Name
Rabbit anti-human C-C motif chemokine 7 polyclonal Antibody, Biotin conjugated
Gene Names
CCL7; FIC; MARC; MCP3; NC28; MCP-3; SCYA6; SCYA7
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot
(Western Blot analysis using LYN antibodyWestern Blot showing LYN antibody agains HL60 (1), L540 (2), SLLP-M2 (3), SEM (4) and Ramos (5) cell lysate.)
LYN, Monoclonal Antibody (Cat# AAA533234)
Full Name
LYN antibody
Applications
EIA, WB
Pricing
$645/0.1 mL | $2,885/5x0.1 mL
Testing Data #1
(Staining of formalin fixed, parafin embedded human placenta with Rabbit anti Human caspase-2 (MBS222887))
CASPASE-2, Polyclonal Antibody (Cat# AAA222887)
Full Name
RABBIT ANTI HUMAN CASPASE-2
Gene Names
CASP2; ICH1; NEDD2; CASP-2; NEDD-2; PPP1R57
Reactivity
Mouse
Applications
WB
Pricing
$610/0.05 mg | $2,595/5x0.05 mg
Western Blot/Blotting
(Western blot analysis using caspase-8 antibody on MCF-7 cells negative (-) and positive (+) for caspase-3 after treatment for 48 hours with thapsigargin. Antibody detects the proenzyme and one of the cleavage products.)
Caspase-8, Monoclonal Antibody (Cat# AAA395036)
Full Name
Caspase-8
Gene Names
CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
Reactivity
Human
Applications
WB
Purity
Protein A/G Chromatography
Pricing
$390/0.1 mg | $1,610/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Interferon gamma ntibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 18.3 kDaObserved band size: 27 kDa Additional bands at: 70kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Interferon gamma, Polyclonal Antibody (Cat# AAA715642)
Full Name
Rabbit anti-human Interferon gamma polyclonal Antibody, HRP conjugated
Gene Names
IFNG; IFG; IFI
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Ubiquitin thioesterase OTUB1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 20 kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Ubiquitin thioesterase OTUB1, Polyclonal Antibody (Cat# AAA719480)
Full Name
Rabbit anti-human Ubiquitin thioesterase OTUB1 polyclonal Antibody, FITC
Gene Names
OTUB1; OTB1; OTU1; HSPC263
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Tax1-binding protein 3 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13kDa Observed band size: 70 kDa Additional bands at: 36 kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Tax1-binding protein 3, Polyclonal Antibody (Cat# AAA715727)
Full Name
Rabbit anti-human Tax1-binding protein 3 polyclonal Antibody, HRP conjugated
Gene Names
TAX1BP3; TIP-1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot Neutrophil gelatinase-associated lipocalin Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size:22 kDaObserved band size: 45 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
NGAL, Polyclonal Antibody (Cat# AAA715519)
Full Name
Rabbit anti-human NGAL polyclonal Antibody, HRP conjugated
Gene Names
LCN2; p25; 24p3; MSFI; NGAL
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Prefoldin subunit 5 antibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 17 kDaObserved band size: 70 kDa Additional bands at: 80kDa. We are unsure as to the identity of these extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Prefoldin subunit 5, Polyclonal Antibody (Cat# AAA715290)
Full Name
Rabbit anti-human Prefoldin subunit 5 polyclonal Antibody, Biotin conjugated
Gene Names
PFDN5; MM1; MM-1; PFD5
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Vesicular, overexpressed in cancer, prosurvival protein 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 19kDa Observed band size: 50kDa Additional bands at: 70kDa, 80kDa; We are unsure as to the identity of these extra bands. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Vesicular, overexpressed in cancer, prosurvival protein 1, Polyclonal Antibody (Cat# AAA715755)
Full Name
Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, HRP conjugated
Gene Names
VOPP1; ECOP; GASP
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Vesicular, overexpressed in cancer, prosurvival protein 1 antibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 19 kDaObserved band size: 50 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Vesicular, overexpressed in cancer, prosurvival protein 1, Polyclonal Antibody (Cat# AAA716030)
Full Name
Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, Biotin conjugated
Gene Names
VOPP1; ECOP; GASP
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot Gamma-aminobutyric acid receptor-associated protein-like 2 Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 13kDaObserved band size: 60 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Gamma-aminobutyric acid receptor-associated protein-like 2, Polyclonal Antibody (Cat# AAA715814)
Full Name
Rabbit anti-human Gamma-aminobutyric acid receptor-associated protein-like 2 polyclonal Antibody, Biotin conjugated
Gene Names
GABARAPL2; ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Histone H2B type 1-C/E/F/G/I antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 14 kDa Observed band size: 70kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Histone H2B type 1-C/E/F/G/I, Polyclonal Antibody (Cat# AAA715495)
Full Name
Rabbit anti-human Histone H2B type 1-C/E/F/G/I polyclonal Antibody, Biotin conjugated
Gene Names
HIST1H2BG; H2B/a; H2BFA; H2B.1A; dJ221C16.8
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Rho guanine nucleotide exchange factor 18 antibody at at 2 ug/ml Lane 1: EC109whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 129 kDa Observed band size: 30 kDa Additional bands at: 60kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Rho guanine nucleotide exchange factor 18, Polyclonal Antibody (Cat# AAA715706)
Full Name
Rabbit anti-human Rho guanine nucleotide exchange factor 18 polyclonal Antibody, Biotin conjugated
Gene Names
ARHGEF18; P114-RhoGEF
Reactivity
Human. Other species are not tested. Please decide the specificity by homology.
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data
(Mouse EL4 thymoma cellswere cultured in the absence(A) or presence (B) of 100 ugmitomycin for 18 hours. Thecells were harvested andstained with ICT'sSR-DEVD-FMK inhibitor probe(red histograms) to detect active caspases 3&7. The data illustrate a low level ofcaspase-3 activity in untreated cells (A) compared to unstained control (bluehistogram); caspase-3 activity is greatly enhanced in the mitomycin treatedcultures (B). Cells were analyzed with a BD LSRII flow cytometer to detectsulforhodamine using the green laser (532nm) and detector D set up with filter610/620 600LP (Dr. Catherine Martone, Yale University).)
SR FLICA Caspase 3 and 7, Assay Kit (Cat# AAA258021)
Full Name
SR FLICA Caspase 3 and 7 Assay Kit
Pricing
$665/100 Tests | $300/25 Tests | $3,010/5x100 Tests
Testing Data
(Jurkat cells were treated with 1 uM staurosporine toinduce caspase 9 activity (left), or a negative control(right) for 3 hours, washed twice, then incubated withICT's red caspase 9 inhibitor probe, SR-LEHD-FMK, for 1hour and examined under a fluorescence microscope.DIC images were also taken of all cells. Both inducedimages reveal several experimental cells which fluorescered, therefore they have some degree of caspase 9activity. The non-induced DIC image reveals many controlcells, however the corresponding fluorescence image isdark; none of these cells have active caspase 9 (Dr. BrianW. Lee, ICT).)
SR-FLICA Caspase 9, Assay Kit (Cat# AAA258030)
Full Name
SR-FLICA Caspase 9 Assay Kit
Pricing
$665/100 Tests | $300/25 Tests | $3,010/5x100 Tests
Testing Data #1
(Staining of formalin fixed, paraffin processed human pancreas with Rabbit anti Human EndoG at 15 ug/ml. (MBS221699))
ENDONUCLEASE G, Polyclonal Antibody (Cat# AAA221699)
Full Name
RABBIT ANTI ENDONUCLEASE G
Reactivity
Mouse (Expected from Sequence), Rat (Expected from Sequence)
Applications
WB
Pricing
$430/0.1 mg | $1,780/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Diamine acetyltransferase 1 antibody at 2/mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 19 kDa Observed band size: 90 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Diamine acetyltransferase 1, Polyclonal Antibody (Cat# AAA719821)
Full Name
Rabbit anti-human Diamine acetyltransferase 1 polyclonal Antibody, HRP conjugated
Gene Names
SAT1; SAT; DC21; KFSD; SSAT; KFSDX; SSAT-1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Caspase-7, Monoclonal Antibody (Cat# AAA395071)
Full Name
Caspase-7
Gene Names
CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
Reactivity
Human
Applications
WB
Purity
Protein A/G Chromatography
Pricing
$390/0.1 mg | $1,610/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Vesicular, overexpressed in cancer, prosurvival protein 1 antibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 19 kDaObserved band size: 50 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Vesicular, overexpressed in cancer, prosurvival protein 1, Polyclonal Antibody (Cat# AAA715678)
Full Name
Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, FITC
Gene Names
VOPP1; ECOP; GASP
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot Neutrophil gelatinase-associated lipocalin Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size:22 kDaObserved band size: 45 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
NGAL, Polyclonal Antibody (Cat# AAA715503)
Full Name
Rabbit anti-human NGAL polyclonal Antibody, FITC conjugated
Gene Names
LCN2; p25; 24p3; MSFI; NGAL
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
FAM-VAD-OPH II in vitro Apoptosis, Detection Reagent (Cat# AAA258039)
Full Name
FAM-VAD-OPH II in vitro Apoptosis Detection Reagent, Pack of 4 Vials
Pricing
$610/4 Vials | $2,685/5x4 Vials
Caspase Family Group II, Active, Recombinant Protein (Cat# AAA636140)
Full Name
Caspase Family Group II, Active, Recombinant (Caspases-2,3,7)
Gene Names
CSNK2A2; CK2A2; CSNK2A1
Purity
Molecular Biology Grade
Pricing
$630/3x25 Units
Testing Data
(HL-60 cells were labeled with FAM-FLISP and ICT's SR-VAD-FMK FLICA caspase reagent (kit#917) after incubation with camptothecin for 3hours. Cells with active serine proteases staingreen (FAM-FLISP, y-axis, Fig. 1) and cellswith active caspases stain red (SR-VAD-FMK,x-axis, Fig. 5). Co-localization of serineprotease activity versus caspase activity isevident in dually stained cells (Figs. 2, 3, 4, &6).The DIC image (Fig. 7) reveals many negativecells (Dr. Zbigniew Darzynkiewicz, BranderCancer Center). )
FAM-Spacer-Leu-CMK Green FLISP, Assay Kit (Cat# AAA258003)
Full Name
FAM-Spacer-Leu-CMK Green FLISP Assay Kit
Pricing
$615/100 Tests | $290/25 Tests | $2,770/5x100 Tests
Western Blot/Blotting
(Western blot analysis)
Caspase-12 (involved in Alzheimers), Polyclonal Antibody (Cat# AAA395486)
Full Name
Caspase-12 (NT) (involved in Alzheimers)
Gene Names
CASP12; CASP-12; CASP12P1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Antigen Immunoaffiinity Purification
Pricing
$610/0.1 mg | $2,585/5x0.1 mg
Western Blot (WB)
(BCL2 Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the BCL2 antibody detected the BCL2 protein (arrow).)
BCL2, Polyclonal Antibody (Cat# AAA9206940)
Full Name
BCL2 Antibody (Center)
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human
Predicted Reactivity: Bovine, Chicken, Hamster, Mouse, Rat
Predicted Reactivity: Bovine, Chicken, Hamster, Mouse, Rat
Applications
WB, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot/Blotting
(Western blot analysis)
Caspase-12, Polyclonal Antibody (Cat# AAA395820)
Full Name
Caspase-12 (IN)
Gene Names
CASP12; CASP-12; CASP12P1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Antigen Immunoaffiinity Purification
Pricing
$610/0.1 mg | $2,585/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Protransforming growth factor alpha antibody at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 18 kDa Observed band size: 30 kDa Additional bands at: 35 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Protransforming growth factor alpha, Polyclonal Antibody (Cat# AAA719498)
Full Name
Rabbit anti-human Protransforming growth factor alpha polyclonal Antibody, FITC
Gene Names
TGFA; TFGA
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Caspase 7, Antibody (Cat# AAA555012)
Full Name
Mouse Monoclonal Anti-Caspase 7
Gene Names
Casp7; MCH3; SCA-2; CASP-7; ICE-LAP3; Caspase-7; MCH3/SCA-2
Reactivity
Human, Mouse, Rat
Applications
WB
Pricing
$425/0.1 mL | $1,760/5x0.1 mL
Western Blot/Blotting
(Western blot analysisusing caspase-8 antibody on MCF-7 cells negative (-) and positive (+) for caspase-3 after treatment for 48 hours with thapsigargin. Antibody detects procaspase and one cleavage product.)
Caspase-8, Monoclonal Antibody (Cat# AAA395261)
Full Name
Caspase-8
Gene Names
CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
Reactivity
Human
Applications
WB
Purity
Protein A/G Chromatography
Pricing
$390/0.1 mg | $1,610/5x0.1 mg
Western Blot (WB)
(Western blot Gamma-aminobutyric acid receptor-associated protein-like 2 Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 13kDaObserved band size: 60 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Gamma-aminobutyric acid receptor-associated protein-like 2, Polyclonal Antibody (Cat# AAA715906)
Full Name
Rabbit anti-human Gamma-aminobutyric acid receptor-associated protein-like 2 polyclonal Antibody, HRP conjugated
Gene Names
GABARAPL2; ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Vesicular, overexpressed in cancer, prosurvival protein 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 19kDa Observed band size: 50kDa Additional bands at: 70kDa, 80kDa; We are unsure as to the identity of these extra bands. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Vesicular, overexpressed in cancer, prosurvival protein 1, Polyclonal Antibody (Cat# AAA715766)
Full Name
Rabbit anti-human Vesicular, overexpressed in cancer, prosurvival protein 1 polyclonal Antibody, Biotin conjugated
Gene Names
VOPP1; ECOP; GASP
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis with Jurkat cell lysate. Lane 1: Untreated;Lane 2: Etoposide treated.)
Active Caspase 9, Polyclonal Antibody (Cat# AAA837333)
Full Name
Active Caspase 9 antibody
Applications
IP, WB
Purity
Active Caspase 9 antibody was purified by affinity chromatography
Pricing
$715/0.1 mg | $3,220/5x0.1 mg
Immunohistochemistry (IHC)
(Human Kidney (formalin-fixed, paraffin-embedded) stained with RNF34 antibody at 3.75ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
RNF34, Polyclonal Antibody (Cat# AAA646965)
Full Name
RNF34, NT (E3 Ubiquitin-protein Ligase RNF34, Caspase Regulator CARP1, Caspases-8 and -10-associated RING Finger Protein 1, CARP-1, FYVE-RING Finger Protein Momo, Human RING Finger Homologous to Inhibitor of Apoptosis Protein, hRFI, RING Finger Protein 34
Gene Names
RNF34; RFI; RIF; RIFF; hRFI; CARP1; CARP-1
Reactivity
Human
Applications
EL/EIA, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$735/0.05 mg | $3,290/5x0.05 mg
Western Blot (WB)
(Western blot All lanes: 60S ribosomal protein L17 ntibody at at 2 /ml + EC109whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 58 kDaObserved band size: 30 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
G-protein coupled receptor 161, Polyclonal Antibody (Cat# AAA1264985)
Full Name
Rabbit anti-human G-protein coupled receptor 161 polyclonal Antibody, FITC
Gene Names
GPR161; RE2
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: C-C motif chemokine 5 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 10 kDa Observed band size: 70 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
C-C motif chemokine 5, Polyclonal Antibody (Cat# AAA719945)
Full Name
Rabbit anti-human C-C motif chemokine 5 polyclonal Antibody, HRP conjugated
Gene Names
CCL5; SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot/Blotting
(Western blot analysis using Caspase-9 (IN1) antibody at 0.5 ug/ml on K562 cell lysate.)
Caspase-9 (IN1) (ICE, LAP6, Mch6, Apaf-3), Polyclonal Antibody (Cat# AAA395529)
Full Name
Caspase-9 (IN1) (ICE, LAP6, Mch6, Apaf-3)
Gene Names
CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
Reactivity
Human
Applications
WB
Purity
Antigen Immunoaffiinity Purification
Pricing
$610/0.1 mg | $2,585/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Endoplasmic reticulum resident protein 29 antibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 29 kDaObserved band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Endoplasmic reticulum resident protein 29, Polyclonal Antibody (Cat# AAA715854)
Full Name
Rabbit anti-human Endoplasmic reticulum resident protein 29 polyclonal Antibody, FITC
Gene Names
ERP29; ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Glutathione S-transferase P antibody at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 23 kDa Observed band size: 30 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Glutathione S-transferase P, Polyclonal Antibody (Cat# AAA719806)
Full Name
Rabbit anti-human Glutathione S-transferase P polyclonal Antibody, Biotin conjugated
Gene Names
GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Metalloproteinase inhibitor 2 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 24kDa Observed band size: 60kDa Additional bands at: 118 kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Metalloproteinase inhibitor 2, Polyclonal Antibody (Cat# AAA715610)
Full Name
Rabbit anti-human Metalloproteinase inhibitor 2 polyclonal Antibody, FITC
Gene Names
TIMP2; DDC8; CSC-21K
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
