Western Blot (WB)
(Western blot Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Antibody at 2 ug/ml + EC109 whole cell lysate at 20 ug Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 34 kDa Observed band size: 45 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Rabbit anti-Human Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Polyclonal Antibody | anti-PPP2CB antibody
Rabbit anti-human Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform polyclonal Antibody, FITC
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Western Blot (WB)
(Western blot Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Antibody at 2 ug/ml + EC109 whole cell lysate at 20 ug Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 34 kDa Observed band size: 45 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Western Blot (WB)
(Western blot Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Antibody at 2 ug/ml + EC109 whole cell lysate at 20 ug Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 34 kDa Observed band size: 45 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. [provided by RefSeq, Mar 2010]
Uniprot Description
PPP2CB: one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits.
Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 8p12
Cellular Component: chromosome, pericentric region; cytosol; nucleus; protein phosphatase type 2A complex; spindle pole
Molecular Function: metal ion binding; protein binding; protein C-terminus binding; protein serine/threonine phosphatase activity
Biological Process: apoptotic mitochondrial changes; fibroblast growth factor receptor signaling pathway; negative regulation of Ras protein signal transduction; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid dephosphorylation; regulation of gene expression; response to antibiotic; response to hydrogen peroxide
Research Articles on PPP2CB
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Product Notes
The PPP2CB ppp2cb (Catalog #AAA715317) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform polyclonal Antibody, FITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the PPP2CB ppp2cb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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