Rabbit anti-Human Caspase 10 Polyclonal Antibody | anti-CASP10 antibody

Caspase 10 antibody

Cat. Num. AAA536248

• Gene Names: CASP10; MCH4; ALPS2; FLICE2
• Reactivity: Human
• Applications: Western Blot
• Purity: Caspase 10 antibody was purified by affinity chromatography.
• Synonyms: Caspase 10; Polyclonal Antibody; Caspase 10 antibody; Polyclonal Caspase 10; Anti-Caspase 10; Cysteine-Aspartic Acid Protease 10; Caspase -10; Caspase-10; anti-CASP10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Caspase 10 antibody was purified by affinity chromatography.
• Form/Format: Affinity purified IgG supplied in PBS with 0.02% NaN3.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 521

• Applicable Applications for anti-CASP10 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1,000
• Biological Significance: Caspases are death effector domain-containing cysteine aspartases presumed to be at or near the apex of apoptotic signaling pathways. All known isoforms of caspase-10 are abundantly expressed in fetal lung, kidney, and skeletal muscle but are very poorly expressed or absent in these tissues in the adult.
• Cross-Reactivity: Avian, Mouse, rat
• Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
• Immunogen: Caspase 10 antibody was raised in rabbit using N terminal sequence MKSQGQHWYSSSDKN of all isoforms of human caspase-10 as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for anti-CASP10 antibody

Affinity purified Rabbit polyclonal Caspase 10 antibody

Product Categories/Family for anti-CASP10 antibody

Cell Cycle & Cell Death; Purified Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 47078269
• NCBI GeneID: 843
• NCBI Accession #: NP_116756.2
• NCBI GenBank Nucleotide #: NM_032974.4
• UniProt Accession #: Q92851; Q68HC0; Q6KF62; Q6KF63; Q8IUP5; Q8WYQ8; Q99845; Q9Y2U6; Q9Y2U7
• Molecular Weight: 28,364 Da
• NCBI Official Full Name: caspase-10 isoform 2 preproprotein
• NCBI Official Synonym Full Names: caspase 10, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP10
• NCBI Official Synonym Symbols: MCH4; ALPS2; FLICE2
• NCBI Protein Information: caspase-10
• UniProt Protein Name: Caspase-10
• Protein Family: Caspase
• UniProt Gene Name: CASP10
• UniProt Synonym Gene Names: MCH4; CASP-10; FLICE2
• UniProt Entry Name: CASPA_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]

Uniprot Description

CASP10: Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit. Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon. Belongs to the peptidase C14A family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.63

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: cytoplasm; plasma membrane; CD95 death-inducing signaling complex; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin protein ligase binding

Biological Process: regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; innate immune response; proteolysis

Disease: Lymphoma, Non-hodgkin, Familial; Gastric Cancer; Autoimmune Lymphoproliferative Syndrome, Type Iia