Western Blot (WB)
(Western blot All lanes: Nucleoside diphosphate kinase A antibody at at 2 /mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size:16.7 kDa Observed band size: 32kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Rabbit anti-Human Nucleoside diphosphate kinase A Polyclonal Antibody | anti-NME1 antibody
Rabbit anti-human Nucleoside diphosphate kinase A polyclonal Antibody, HRP conjugated
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Western Blot (WB)
(Western blot All lanes: Nucleoside diphosphate kinase A antibody at at 2 /mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size:16.7 kDa Observed band size: 32kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Western Blot (WB)
(Western blot All lanes: Nucleoside diphosphate kinase A antibody at at 2 /mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size:16.7 kDa Observed band size: 32kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
NCBI and Uniprot Product Information
NCBI Description
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]
Uniprot Description
NME1: an enzyme with multiple activities including histidine protein kinase, nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase and 3'-5' exonuclease activities. Plays a major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Involved in cell proliferation, differentiation and development, G protein-coupled receptor endocytosis, cytotoxic T cell and NK cell-mediated cell death, and gene expression. Required for neural development including neural patterning and cell fate determination. During granzyme A (GZMA)-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Three alternatively spliced human proteins have been reported.
Protein type: Nucleotide Metabolism - pyrimidine; Tumor suppressor; Protein kinase, histidine; Kinase, nucleoside diphosphate; Nucleotide Metabolism - purine; Kinase, other; EC 2.7.4.6; Other group; NDK family
Chromosomal Location of Human Ortholog: 17q21.3
Cellular Component: centrosome; cytoplasm; cytosol; intermediate filament; membrane; mitochondrial outer membrane; myelin sheath; nucleus; perinuclear region of cytoplasm
Molecular Function: ATP binding; deoxyribonuclease activity; gamma-tubulin binding; GTP binding; identical protein binding; intermediate filament binding; magnesium ion binding; nucleoside diphosphate kinase activity; protein binding; protein kinase binding; ribosomal small subunit binding; single-stranded DNA binding
Biological Process: CTP biosynthetic process; DNA metabolic process; endocytosis; GTP biosynthetic process; hippocampus development; lactation; negative regulation of cell proliferation; negative regulation of myeloid leukocyte differentiation; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; nucleoside diphosphate phosphorylation; nucleoside triphosphate biosynthetic process; positive regulation of DNA binding; positive regulation of epithelial cell proliferation; purine nucleotide metabolic process; pyrimidine nucleotide metabolic process; regulation of apoptosis; response to amine stimulus; response to cAMP; response to testosterone stimulus; UTP biosynthetic process
Disease: Neuroblastoma, Susceptibility To
Research Articles on NME1
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Product Notes
The NME1 nme1 (Catalog #AAA715318) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Nucleoside diphosphate kinase A polyclonal Antibody, HRP conjugated reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleoside diphosphate kinase A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the NME1 nme1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleoside diphosphate kinase A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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