Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "Goat IgG Fc " - showing 1150-1200
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215793)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
Flow Cytometry
(Overlay histogram showing Jurkat cells stained with MBS9211489 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9211489, 1:25 dilution) for 60 min at 37ºC.The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
NETO2, Polyclonal Antibody (Cat# AAA9211489)
Full Name
NETO2 Antibody (N-term)
Gene Names
NETO2; BTCL2; NEOT2
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysates from A431, HepG2, LNCaP cell line and mouse testis, rat testis tissue lysates (from left to right), using TPI1 Antibody (C-term). MBS9208901 was diluted at 1:1000 at each lane.A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
TPI1, Polyclonal Antibody (Cat# AAA9208901)
Full Name
TPI1 Antibody (C-term)
Gene Names
TPI1; TIM; TPI; TPID; HEL-S-49
Reactivity
Human, Mouse, Rat (Predicted Reactivity: Bovine, Monkey, Rabbit)
Applications
IHC-p, FC, WB, EIA
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC)-Formallin
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941).)
ROR-gamma/RORC, Monoclonal Antibody (Cat# AAA4381544)
Full Name
ROR-gamma/RORC (RAR-related Orphan Receptor C)
Gene Names
RORC; TOR; RORG; RZRG; IMD42; NR1F3; RZR-GAMMA
Reactivity
Human
Applications
FC, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using PRKACA antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
PKA C, Polyclonal Antibody (Cat# AAA125085)
Full Name
PKA C
Gene Names
PRKACA; PKACA
Reactivity
Human, mouse, rat
Applications
WB, IHC, ICC, IP, FC/FACS
Pricing
$180/0.02 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse anti Pig CD4 . All experiments performed on frozen porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
CD4, Monoclonal Antibody (Cat# AAA225686)
Full Name
Mouse Anti Pig CD4
Reactivity
Pig
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Pricing
$355/0.1 mg | $1,435/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast cancer using MBS7602450 (PCNA antibody) at dilution of 1:800)
PCNA, Monoclonal Antibody (Cat# AAA7602450)
Full Name
PCNA Mouse Monoclonal
Gene Names
PCNA; ATLD2
Reactivity
Human, Mouse
Applications
EIA, WB, IHC, FC/FACS, IP
Purity
>=95% as determined by SDS-PAGE
Protein A+G purification
Protein A+G purification
Pricing
$320/0.1 mg | $1,360/5x0.1 mg
Testing Data
(Figure A. RPE conjugated Mouse anti Pig CD3 and FITC conjugated Mouse IgG2a isotype control . Figure B. RPE conjugated Mouse anti Pig CD3 and FITC conjugated Mouse anti Pig CD8 alpha . All experiments performed on porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
CD8 alpha, Monoclonal Antibody (Cat# AAA225688)
Full Name
Mouse Anti Pig CD8 alpha
Reactivity
Pig
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Pricing
$355/0.1 mg | $1,435/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using S100A6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
S100A6, Polyclonal Antibody (Cat# AAA129056)
Full Name
S100A6 Polyclonal Antibody
Gene Names
RPL19; L19
Reactivity
Human, Mouse
Applications
WB, IHC, IF, FC/FACS
Purity
Affinity Purification
Pricing
$210/0.02 mL | $265/0.05 mL | $380/0.1 mL | $540/0.2 mL | $2,285/5x0.2 mL
Testing Data #1
(Staining of equine peripheral blood granulocytes with Mouse anti Horse CD13 (MBS224706) followed by Goat anti Mouse IgG:FITC (MBS235142))
CD13, Monoclonal Antibody (Cat# AAA224545)
Full Name
MOUSE ANTI HORSE CD13:FITC
Applications
FC/FACS
Pricing
$570/0.1 mg | $2,405/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HepG2 cells using FABP3(green) compared to an isotype control of rabbit IgG(blue). MBS9203654 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
FABP3, Polyclonal Antibody (Cat# AAA9203654)
Full Name
FABP3 Antibody
Gene Names
FABP3; MDGI; FABP11; H-FABP; M-FABP; O-FABP
Reactivity
Human
Applications
FC/FACS, EIA, IHC, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27 detected with Goat anti Mouse IgG:FITC , and Mouse anti Pig wCD8a:RPE )
CD27, Monoclonal Antibody (Cat# AAA224793)
Full Name
MOUSE ANTI PIG CD27: FITC
Gene Names
CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
Reactivity
Pig
Applications
FC/FACS
Pricing
$560/0.1 mg | $2,365/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MCF-7 cells using AGAP8 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9206649 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
AGAP8, Polyclonal Antibody (Cat# AAA9206649)
Full Name
AGAP8 Antibody (C-term)
Reactivity
Human
Applications
WB, EIA, FC/FACS, IF, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PACSIN3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
PACSIN3, Polyclonal Antibody (Cat# AAA9130488)
Full Name
PACSIN3 Polyclonal Antibody
Gene Names
PACSIN3; SDPIII
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, FC/FACS
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunohistochemistry (IHC)
(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
HOXA3, Polyclonal Antibody (Cat# AAA2401819)
Full Name
Anti-HOXA3 Antibody (aa413-440)
Gene Names
HOXA3; HOX1; HOX1E
Reactivity
Human
Applications
FC/FACS, IHC, WB
Purity
Protein A purified
Pricing
$585/0.2 mL | $2,585/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CACYBP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
CACYBP, Polyclonal Antibody (Cat# AAA9129195)
Full Name
CACYBP Polyclonal Antibody
Gene Names
CACYBP; SIP; GIG5; PNAS-107; S100A6BP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP, FC/FACS
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Testing Data #1
(Detection of Mouse anti Rabbit CD44 (MBS224526) in a flow cytometric analysis of rabbit peripheral blood lymphocytes using Goat anti Rabbit IgG H+L:FITC (MBS235142))
IgG, Polyclonal Antibody (Cat# AAA235247)
Full Name
GOAT ANTI MOUSE IgG (H/L) (MULTI SPECIES ADSORBED)
Applications
EIA, FC/FACS, WB
Pricing
$275/0.5 mg | $1,085/5x0.5 mg
Testing Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody (MBS235406) for detection. Low power)
CD43, Monoclonal Antibody (Cat# AAA216464)
Full Name
MOUSE ANTI RAT CD43:FITC
Gene Names
Spn; Lsn; Cd43; Lsn1; gpL115
Applications
FC/FACS
Pricing
$425/0.1 mg | $1,755/5x0.1 mg
Testing Data
(1:1000 dilution of this antibody detected SMAD5 on 40ug of cell lysates)
SMAD5, Monoclonal Antibody (Cat# AAA9400137)
Full Name
SMAD5(C-term) Monoclonal Antibody
Gene Names
SMAD5; DWFC; JV5-1; MADH5
Reactivity
Human, Porcine, Rat, Mouse
Applications
WB, ICC, FC/FACS
Purity
Purified by antigen-affinity chromatography.
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from mouse brain tissue, rat H-4-II-E,mouse NIH/3T3 cell line and rat brain tissue lysate(from left to right), using STIP1 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
STIP1, Polyclonal Antibody (Cat# AAA9231720)
Full Name
STIP1 Antibody (C-term)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Hamster, Monkey
Predicted: Bovine, Hamster, Monkey
Applications
WB, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(All lanes : Anti-WDR43 Antibody (C-term) at 1:1000 dilutionLane 1: Jurkat whole cell lysateLane 2: K562 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: human liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WDR43, Polyclonal Antibody (Cat# AAA9232135)
Full Name
WDR43 Antibody (C-term)
Gene Names
WDR43; UTP5; NET12
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
DDX58, Antibody (Cat# AAA423724)
Full Name
Goat anti-DDX58 Antibody
Gene Names
DDX58; RIGI; RIG-I; RLR-1; SGMRT2
Reactivity
Human
Expected from sequence similarity: Mouse
Expected from sequence similarity: Mouse
Applications
EIA, IF, FC/FACS
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
(All lanes : SF3B3 Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction as negative control Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 135 KDa Observed band size : 135 KDa Blocking/Dilution buffer : 1x TBST.)
SF3B3, Monoclonal Antibody (Cat# AAA435135)
Full Name
SF3B3 Mouse mAb
Gene Names
SF3B3; RSE1; SAP130; SF3b130; STAF130
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from MCF-7, NCI-H460 cell line (from left to right), using TAZ Antibody (N-term). MBS9210817 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
TAZ, Polyclonal Antibody (Cat# AAA9210817)
Full Name
TAZ Antibody (N-term)
Gene Names
TAZ; EFE; BTHS; EFE2; G4.5; Taz1; CMD3A; LVNCX
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysates from A431,Hela cell line (from left to right), using SFN Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
SFN, Polyclonal Antibody (Cat# AAA9231736)
Full Name
SFN Antibody (C-term)
Gene Names
SFN; YWHAS
Reactivity
Human
Predicted: Mouse, Bovine
Predicted: Mouse, Bovine
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC (MBS211022))
CD3, Monoclonal Antibody (Cat# AAA211260)
Full Name
MOUSE ANTI HUMAN CD3:Low Endotoxin
Gene Names
CD3G; T3G; IMD17; CD3-GAMMA
Applications
FC/FACS
Pricing
$415/0.5 mg | $1,710/5x0.5 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using XRCC6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)
XRCC6, Polyclonal Antibody (Cat# AAA125856)
Full Name
XRCC6 Polyclonal Antibody
Gene Names
XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, IF, FC/FACS
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(All lanes : Anti-IGF2BP2 Antibody (C-term) at 1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: Hela whole cell lysateLane 3: A549 whole cell lysateLane 4: Raji whole cell lysateLane 5: SH-SY5Y whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
IGF2BP2, Polyclonal Antibody (Cat# AAA9232158)
Full Name
IGF2BP2 Antibody (C-term)
Gene Names
IGF2BP2; IMP2; IMP-2; VICKZ2
Reactivity
Human
Predicted: Mouse
Predicted: Mouse
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-TGF-alpha antibody tAb2 (Ab00614) Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of tAb2 (Ab00614-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
TGFalpha, Monoclonal Recombinant Antibody (Cat# AAA488330)
Full Name
Anti-TGFalpha [tAb2]
Gene Names
TGFA; TFGA
Reactivity
Human
Applications
XRC, ITC, DB, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC (MBS211022))
CD3, Monoclonal Antibody (Cat# AAA213832)
Full Name
MOUSE ANTI HUMAN CD3:RPE
Gene Names
CD3G; T3G; IMD17; CD3-GAMMA
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Flow Cytometry (FC/FACS)
(Overlay histogram showing Hela cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was mouse IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
EGFR, Monoclonal Antibody (Cat# AAA9231961)
Full Name
EGFR Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
WB, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808801)
Full Name
Methylglyoxal Antibody, Clone 9E7: Biotin
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,255/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
4-Hydroxy-2-hexenal, Monoclonal Antibody (Cat# AAA808684)
Full Name
4-Hydroxy-2-hexenal Antibody, Clone 6F10: ATTO 488
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA, FC/FACS
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dityrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Dityrosine, Monoclonal Antibody (Cat# AAA808889)
Full Name
Dityrosine Antibody, Clone 10A6: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10 Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of LG.3A10 (MBS488455) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488455, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD27, Monoclonal Recombinant Antibody (Cat# AAA488455)
Full Name
Anti-CD27 [LG.3A10]
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Applications
IP, IF, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Hexanoyl-Lysine adduct, Monoclonal Antibody (Cat# AAA808658)
Full Name
Hexanoyl-Lysine adduct Antibody, Clone 5D9: FITC
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,235/5x0.1 mg
Western Blot (WB)
(Western BlotPositive WB detected in: A375 whole cell lysateAll lanes: CD146 antibody at 0.6ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 72 KDaObserved band size: 120 KDa)
CD146, Monoclonal Recombinant Antibody (Cat# AAA7110755)
Full Name
CD146 Antibody
Gene Names
MCAM; CD146; MUC18
Reactivity
Human
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
Affinity-chromatography
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of SLK using anti-SLK antibody (MBS1750680).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,Lane 2: human PANC-1 whole cell lysates,Lane 3: human 22RV1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLK antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLK at approximately 200KD. The expected band size for SLK is at 143KD. )
SLK, Polyclonal Antibody (Cat# AAA1750680)
Full Name
Anti-SLK antibody
Gene Names
SLK; LOSK; STK2; se20-9; bA16H23.1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha: Azide Free (MBS210176))
CD8, Monoclonal Antibody (Cat# AAA212306)
Full Name
RAT ANTI MOUSE CD8
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
FC/FACS
Pricing
$475/0.25 mg | $1,995/5x0.25 mg
Western Blot (WB)
(Western blot analysis of extracts of A-549 cells, using MSI1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Musashi, Polyclonal Antibody (Cat# AAA126454)
Full Name
Musashi
Reactivity
Human, rat
Applications
WB, IHC, ICC, IP, FC/FACS
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ICAM3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
ICAM3, Polyclonal Antibody (Cat# AAA129604)
Full Name
ICAM3 Polyclonal Antibody
Gene Names
ICAM3; CD50; CDW50; ICAM-R
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using EZR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Ezrin, Polyclonal Antibody (Cat# AAA125879)
Full Name
Ezrin
Gene Names
EZR; CVL; CVIL; VIL2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IP, FC/FACS
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RPA1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
RPA70, Polyclonal Antibody (Cat# AAA125356)
Full Name
RPA70
Gene Names
RPA1; HSSB; RF-A; RP-A; REPA1; RPA70; MST075
Reactivity
Human
Applications
WB, IHC, ICC, IP, FC/FACS
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Testing Data
(Staining of dog peripheral lymphocytes with Rat anti Dog CD5:Alexa Fluor488 )
CD5, Monoclonal Antibody (Cat# AAA219892)
Full Name
RAT ANTI DOG CD5
Applications
FC/FACS, IP
Pricing
$395/0.1 mg | $1,630/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using KCNIP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
KCNIP1, Polyclonal Antibody (Cat# AAA129249)
Full Name
KCNIP1 Polyclonal Antibody
Gene Names
KCNIP1; VABP; KCHIP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
SDS-Page
(Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human placenta tissue lysate, Primary antibody:47549(FCGRT Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
FCGRT, Polyclonal Antibody (Cat# AAA9430531)
Full Name
FCGRT Antibody
Gene Names
FCGRT; FCRN; alpha-chain
Reactivity
Human
Applications
WB, IHC
Purity
Antigen affinity purification
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Immunohistochemistry (IHC)
(Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))
MT-ND5, Polyclonal Antibody (Cat# AAA2401667)
Full Name
Anti-MT-ND5 Antibody (aa544-570)
Gene Names
MT-ND5; MTND5; ND5
Reactivity
Human
Applications
FC/FACS, IHC, WB
Purity
Protein A purified
Pricing
$585/0.2 mL | $2,585/5x0.2 mL
Western Blot (WB)
((0.1ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
EHD1, Antibody (Cat# AAA423832)
Full Name
Goat Anti-EHD1 Antibody
Gene Names
EHD1; PAST; PAST1; H-PAST; HPAST1
Reactivity
Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow
Expected from sequence similarity: Human, Mouse, Rat, Cow
Applications
EIA, IF, IHC, FC/FACS
Pricing
$335/0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SEPT9 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
SEPT9, Polyclonal Antibody (Cat# AAA9129475)
Full Name
SEPT9 Polyclonal Antibody
Gene Names
SEPT9; MSF; MSF1; NAPB; SINT1; PNUTL4; SeptD1; AF17q25
Reactivity
Human
Applications
WB, IHC, IF, IP, FC/FACS
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of lysates from Raji,Ramos,Daudi cell line (from left to right), using CHRNA9 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
CHRNA9, Polyclonal Antibody (Cat# AAA9231859)
Full Name
CHRNA9 Antibody (N-term)
Gene Names
CHRNA9; NACHRA9; HSA243342
Reactivity
Human
Predicted: Rat
Predicted: Rat
Applications
WB, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
