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Immunohistochemistry (IHC) (Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))

Rabbit anti-Human MT-ND5 Polyclonal Antibody | anti-MT-ND5 antibody

Anti-MT-ND5 Antibody (aa544-570)

Gene Names
MT-ND5; MTND5; ND5
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Protein A purified
Synonyms
MT-ND5; Polyclonal Antibody; Anti-MT-ND5 Antibody (aa544-570); Rabbit Polyclonal (IgG) to Human MT-ND5; MTND5; NAD5; NADH dehydrogenase 5; NADH dehydrogenase subunit 5; ND5; NADH5; anti-MT-ND5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
This ND5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 544-570 amino acids from the C-terminal region of human ND5.
Purity/Purification
Protein A purified
Form/Format
PBS, 0.09% sodium azide
Sequence Length
603
Applicable Applications for anti-MT-ND5 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
FC/FACS: 1:10-1:50
IHC-P: 1:50-1:100
WB: 1:1000
Conjugation
Unconjugated
Epitope
aa544-570
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC) (Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot of ND5 Antibody in CEM cell line lysates (35 ug/lane). ND5 (arrow) was detected using the purified antibody.)

Western Blot (WB) (Western blot of ND5 Antibody in CEM cell line lysates (35 ug/lane). ND5 (arrow) was detected using the purified antibody.)

Flow Cytometry (FC/FACS)

(Flow cytometric of CEM cells using ND5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric of CEM cells using ND5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-MT-ND5 antibody
Core subunit of the mitochondrial membrane respiratory chain NADHdehydrogenase (Complex I) that is believed to belong to the minimalassembly required for catalysis. Complex I functions in the transfer ofelectrons from NADH to the respiratory chain. the immediate electronacceptor for the enzyme is believed to be ubiquinone.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67,027 Da
NCBI Official Full Name
NADH dehydrogenase subunit 5 (mitochondrion)
NCBI Official Synonym Full Names
mitochondrially encoded NADH dehydrogenase 5
NCBI Official Symbol
MT-ND5
NCBI Official Synonym Symbols
MTND5; ND5
NCBI Protein Information
NADH dehydrogenase, subunit 5 (complex I)
UniProt Protein Name
NADH-ubiquinone oxidoreductase chain 5
UniProt Gene Name
MT-ND5
UniProt Synonym Gene Names
MTND5; NADH5; ND5

Uniprot Description

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone ().

Research Articles on MT-ND5

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Product Notes

The MT-ND5 mt-nd5 (Catalog #AAA2401667) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MT-ND5 Antibody (aa544-570) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MT-ND5 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). FC/FACS: 1:10-1:50 IHC-P: 1:50-1:100 WB: 1:1000. Researchers should empirically determine the suitability of the MT-ND5 mt-nd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MT-ND5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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