Western Blot (WB)
((0.1ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Goat EHD1 Antibody | anti-EHD1 antibody
Goat Anti-EHD1 Antibody
Expected from sequence similarity: Human, Mouse, Rat, Cow
Expected from sequence similarity: Human, Mouse, Rat, Cow
IHC: Paraffin embedded Human Thymus and Brain (Cortex). Recommended concentration: 3.75ug/ml.
IF: Strong expression of the protein seen in the membranes and cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.
FC/FACS: Flow cytometric analysis of HeLa cells.
Western Blot (WB)
((0.1ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Immunohistochemistry (IHC)
((3.75ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.75ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]
Uniprot Description
EHD1: Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes.
Protein type: Calcium-binding; Vesicle
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: membrane; recycling endosome membrane; early endosome membrane; plasma membrane; lipid particle; endosome membrane
Molecular Function: protein binding; GTP binding; calcium ion binding; ATP binding; Rab GTPase binding
Biological Process: intracellular protein transport; cholesterol homeostasis; endocytosis; blood coagulation; endocytic recycling; neurite development; protein homooligomerization
Research Articles on EHD1
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Product Notes
The EHD1 ehd1 (Catalog #AAA423832) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-EHD1 Antibody reacts with Human, Mouse Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's EHD1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), WB, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Peptide ELISA: antibody detection limit dilution 1:32000 IHC: Paraffin embedded Human Thymus and Brain (Cortex). Recommended concentration: 3.75ug/ml. IF: Strong expression of the protein seen in the membranes and cytoplasm of HeLa cells. Recommended concentration: 10ug/ml. FC/FACS: Flow cytometric analysis of HeLa cells. Researchers should empirically determine the suitability of the EHD1 ehd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EHD1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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