Western Blot (WB)
(All lanes : Anti-WDR43 Antibody (C-term) at 1:1000 dilutionLane 1: Jurkat whole cell lysateLane 2: K562 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: human liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human WDR43 Polyclonal Antibody | anti-WDR43 antibody
WDR43 Antibody (C-term)
Gene Names
WDR43; UTP5; NET12
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Synonyms
WDR43; Polyclonal Antibody; WDR43 Antibody (C-term); WD repeat-containing protein 43; KIAA0007; UTP5; anti-WDR43 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This WDR43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636-665 amino acids from the C-terminal region of human WDR43.
Sequence Length
677
Applicable Applications for anti-WDR43 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
WB: 1:1000
IHC-P: 1:50-100
FC/FACS: 1:10-50
IHC-P: 1:50-100
FC/FACS: 1:10-50
Antigen Source
Human
Antigen Region
636-665
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-WDR43 Antibody (C-term) at 1:1000 dilutionLane 1: Jurkat whole cell lysateLane 2: K562 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: human liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-WDR43 Antibody (C-term) at 1:1000 dilutionLane 1: Jurkat whole cell lysateLane 2: K562 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: human liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)-Paraffin
(WDR43 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human Cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR43 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)-Paraffin
(WDR43 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human Cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR43 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(WDR43 Antibody (C-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(WDR43 Antibody (C-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-WDR43 antibody
Description: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Background: The function of WDR43 remains unknown.
Background: The function of WDR43 remains unknown.
References
Olsen, J.V., et al. Cell 127(3):635-648(2006). Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006). Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
74891
NCBI Official Full Name
WD repeat-containing protein 43
NCBI Official Synonym Full Names
WD repeat domain 43
NCBI Official Symbol
WDR43
NCBI Official Synonym Symbols
UTP5; NET12
NCBI Protein Information
WD repeat-containing protein 43
UniProt Protein Name
WD repeat-containing protein 43
Protein Family
UniProt Gene Name
WDR43
UniProt Synonym Gene Names
KIAA0007; UTP5
UniProt Entry Name
WDR43_HUMAN
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Product Notes
The WDR43 wdr43 (Catalog #AAA9232135) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WDR43 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WDR43 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), ELISA (EIA). WB: 1:1000 IHC-P: 1:50-100 FC/FACS: 1:10-50. Researchers should empirically determine the suitability of the WDR43 wdr43 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WDR43, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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