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Testing Data
(Staining of dog peripheral lymphocytes with Rat anti Dog CD5:Alexa Fluor488 )
Rat CD5 Monoclonal Antibody | anti-CD5 antibody
RAT ANTI DOG CD5
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
CD5; Monoclonal Antibody; RAT ANTI DOG CD5; anti-CD5 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
YKIX322.3
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
463
Applicable Applications for anti-CD5 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Dog
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of dog peripheral lymphocytes with Rat anti Dog CD5:Alexa Fluor488 )
Testing Data
(Staining of dog peripheral lymphocytes with Rat anti Dog CD5:Alexa Fluor488 )
Testing Data
(Staining of dog peripheral lymphocytes with Rat anti Dog CD5 followed by Goat F(ab')2 anti Rat IgG:FITC (MBS235148))
Testing Data
(Staining of dog peripheral lymphocytes with Rat anti Dog CD5 followed by Goat F(ab')2 anti Rat IgG:FITC (MBS235148))
Testing Data
(Staining of dog peripheral lymphocytes with Rat anti Dog CD5:Alexa Fluor647 )
Testing Data
(Staining of dog peripheral lymphocytes with Rat anti Dog CD5:Alexa Fluor647 )
Testing Data
(Staining of dog peripheral lymphocytes with Rat anti Dog CD5:APC (MBS219453))
Testing Data
(Staining of dog peripheral lymphocytes with Rat anti Dog CD5:APC (MBS219453))
Related Product Information for anti-CD5 antibody
Rat anti Dog CD5 antibody, clone YKIX322.3 recognizes canine CD5, a 67 kDa cell surface type 1 transmembrane glycoprotein also known as lymphocyte antigen T1, Ly-1 or Leu-1. CD5 is expressed on the surface of T-cells and thymocytes, CD5 is also expressed by NK cells at low levels (Huang et al. 2008). Rat anti dog CD5, cloneYKIX322.3 was clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994). In a study of 73 cases of canine chronic lymphocytic leukemia (CLL) CD5 expression was absent on all cases of B-cell CLL as defined by CD21 expression and lack of CD3 or other T cell antigen expression (Vernau and Moore 1999). Rat anti dog CD5 serves as a useful marker for the discrimination of canine leukemias of differing origins (Villiers 2002).
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
51,130 Da
NCBI Official Full Name
CD5 molecule, partial
UniProt Protein Name
CD5 molecule
Protein Family
UniProt Gene Name
CD5
UniProt Entry Name
H6D9Y3_HUMAN
Similar Products
Product Notes
The CD5 cd5 (Catalog #AAA219892) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD5 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the CD5 cd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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