Western Blot (WB)
(All lanes : SF3B3 Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction as negative control Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 135 KDa Observed band size : 135 KDa Blocking/Dilution buffer : 1x TBST.)
Mouse anti-Human SF3B3 Monoclonal Antibody | anti-SF3B3 antibody
SF3B3 Mouse mAb
IP: 10 tests
FC/FACS: 1:100-1:200
Western Blot (WB)
(All lanes : SF3B3 Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction as negative control Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 135 KDa Observed band size : 135 KDa Blocking/Dilution buffer : 1x TBST.)
Western Blot (WB)
(All lanes : SF3B3 Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction as negative control Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 135 KDa Observed band size : 135 KDa Blocking/Dilution buffer : 1x TBST.)
Immunoprecipitation (IP)
(SF3B3 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25216 of 10 ug. Western blot was performed from the immunoprecipitate using M25216 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)
Immunoprecipitation (IP)
(SF3B3 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25216 of 10 ug. Western blot was performed from the immunoprecipitate using M25216 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling SF3B3 with M25216 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling SF3B3 with M25216 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008]
Uniprot Description
Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome (PubMed:10490618, PubMed:10882114, PubMed:27720643, PubMed:28781166). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077).
Research Articles on SF3B3
Similar Products
Product Notes
The SF3B3 sf3b3 (Catalog #AAA435135) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SF3B3 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SF3B3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:5000 IP: 10 tests FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the SF3B3 sf3b3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SF3B3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
